The paper highlights the data on agroecological assessment and land typing on the example of farm “Kremlin” located in Kochenevo district of Novosibirsk region. This method aims at solving two problems. The first problem deals with typing and it is carried out on the basis of the associated analysis of triunity which implies environmental conditions of the area, agricultural crops, and agricultural technology; the second problem deals with topology: the boundaries of spatial plots of land types coincide with the boundaries of geochemical positions, which are characterized by external features of the relief elements and soil contours. Agroecological land typing includes grouping of lands and their mapping. The criterion for land grouping is seen in similarity of factors that restrict certain crop yields and application of certain technological methods. The authors suggest to use morphometric parameters of the soil mantle for identifying land types: average number of permeable intervals in the section of soil areal, contrast ratio, complexity coefficient and soil cover heterogeneity coefficient. A quantitative parameter of agroecological conditions similarity within the plot is lower contrast ratio. The efficiency of this method is confirmed by economic parameters. Digital mapping and quantitative analysis of morphometric soil mantle parameters was carried out by means of MapInfo Professional software.

The solonetz soil cover 21.7 % of Novosibirsk Region, or 3 686.2 thousand hectares. In the Barabinsk lowland and northern Kulunda, solonetz soil is seen as agricultural land, as it doesn’t make solid massifs, but occur as small spots among zonal soils (black soils, meadow-black soils and black earth-meadow soils). Regardless the vast area, solonetz soils fulfil 20 - 25 % only of the needs of the livestock industry, which is caused by low productivity in the natural state (1.0 - 3.0 c/ha of dry matter in the steppe and 3.0 - 5.0 c/ha in the forest steppe). The authors outline the necessity to increase solonetz complexes fertility and therefore improve precision technologies of farming based on various methods of land reclamation such as chemical, agrotechnical and phytomelioration with adaptive-landscape farming systems that preserve soil fertility and crop yields. Agrobiological reclamation is based on layer-by-layer soil treatment performed once per rotation. This treatment includes milling or distillation of the upper solonetz layer with further deep nonmoldboard loosening, as well as application of dry resistant, salt resistant and solonetz resistant annual and perennial grasses into the crop rotation. These grasses extract easily soluble salts and ash elements from the soil. The research results show the effect of agrobiological melioration on solonetz soils of the Barabinsk lowland. The authors observed the decrease in the number of salts in the soil profile. Particularly, the number of carbonates and hydrocarbons decreased; that indicates slight effect of soda in the soil formation. The researchers observed variation in pH from 9.1 (in virgin variant) to 8.1 – 8.5 influenced by reclamation. Graded tillage and phytomeliorants applied in the crop rotation resulted in higher fertility of saline soils and their chemical parameters equal to the values of zone soil.

The research was carried out on the carbonate black soil of Akmola region in two five-field crop rotations in 2010-2016. The scheme of the experiment included the following ways of soil tillage: deep flat-cutting DF-3-5 (at a depth of 25-27 cm), surface flat-cutting KPSSH-9 (at 10-12 cm), para ploughing SHR-4.5 (at 25-27 cm) and No-till . The researchers observed the highest moisture reserves in the soil layer of 0-100 cm for 7 years in the variants with deep flat-cutting tillage and para ploughing: 115.6 and 112.6 on steam, 108.5 and 105.4 mm on pea. The lowest amount of moisture (80.4 mm) was observed in No-till treatment. The density of the arable soil layer of the southern carbonate black soil before spring wheat sowing corresponded to the appropriate values. When experiencing mechanical treatment, the density of arable layer varied from 1.23 to 1.26 g/cm3; in No-till variant it was 1.31 g/cm3. The concentration of valuables in the variants with deep and surface mechanical tillage varied within 70.9-75.6%, when No-till method was applied, the parameter reduced to 64.5 - 61.5%. The yields of spring wheat sown on steam varied within 1.70 - 1.82 t/ha and did not depend on the soil tillage., Regardless the soil treatment, cultivation of wheat after peas reduced grain harvest on 0.12 - 0.27 t/ha in comparison with steam variant. The authors didn’t observe reliable differences in the crop yield between the first and second crops of grain-steamed crop rotation when applying mechanical soil tillage. In the No-till system there was a reliable decrease in wheat yield on 0.24 t/ha.

The paper highlights the results of many-year (1982-1992, 2011-2014 гг.) research on the concentration of mineral nitrogen in the arable medium peat soil layer of the forest-steppe of Trans Urals zone. The research was carried out on the experimental-reclamation system Reshetnikovo located in the central part of the Tarman bog on the second lacustrine-alluvial basin of the Tura River in Tyumen region. The soils on the experimental plot have a slightly acidic reaction (5.2 - 5.9), relatively low hydrolytic acidity (28.1 - 40.8 mg-eqv/100 g of soil), relatively low degree of base saturation (61.7 - 75.5%), high gross nitrogen concentration (3.1 - 3.9%), low concentration of phosphorus (0.09 - 0.14%) and potassium (0.02 - 0.05%). The researchers found out that mineralization of peat is slow and almost always fails to provide sufficient and continuous supply of nitrogen, despite its large gross reserves. Due to insufficient number of mobile nitrogen compounds from peat organic matter during the growing season it is necessary to apply nitrogen fertilizers. When peat soils contain low concentrations of phosphorus (0.7 - 3.7 mg/100 g of soil), the highest number of nitrate nitrogen is accumulated in the arable layer under perennial grasses due to its low consumption for the yield. Re-reclaimed peat soil is characterized by negative nitrogen concentration. As perennial grasses require nitrogen, it is fulfilled by means of fertilizers on 41.4 - 72%. The authors observed a tendency of reducing nitrate reserves under perennial grasses even if nitrogen fertilizers are applied. This indicates a decrease in the mineralization rate of peat organic matter. The ammonium nitrogen concentration increases. Discontinuance of nitrogen fertilizers use reduces the nitrate nitrogen concentration on 30-49% in the first year and on 46.7-59.1% by the end of the fourth year of after-effect. The amount of ammonium nitrogen increases in 1.6-3.7 times in 4 years.

Adaptibility, plasticity, stability and stress resistance of the oats variety are seen as significant for obtaining stable oat yields with high grain quality. The research aims at assessment of the adaptive capacities of oat varieties selected at Omsk Agricultural Research Centre. The assessment was carried out by means of statistical parameters calculated on the basis of protein concentration in the grain. The experiment was conducted in 2011-2016 on the experimental fields of Omsk Agricultural Research Centre which is located in the southern forest-steppe of Omsk. Protein concentration in the oat grains averaged 11.2% in chaffy varieties and 16.2% in huskless varieties. Following S.A. Eberhart and W.A. Russell assessment, such varieties as Orion, Irtysh 13, Irtysh 21, Irtysh 23, Tarskiy 2, Fakel and Sibirskiy Gerkules combined high stability and response to better environmental conditions. Sibirskiy golozernyy, Progress, Skakun and Levsha varieties are characterized as varieties that responded to the conditions of cultivation (following S.A. Eberhart and W.A. Russell the coefficient of linear regression was 1.06 ÷ 1.72; according to V.A. Dragavtsev the coefficient of multiplicity varied from 2.05 to 2.60). The authors observed high resistant varieties (-1.60 ÷ -2.47) as Pamyati Bogachkova, Irtysh 13, Irtysh 23, Tarskiy 2, Skakun and Irtysh 21. Levsha, Sibirskiy golosernyy and Progress varieties were characterized by high degree of correspondence between environmental factors and genotype (according to A.A. Rossielle and J. Hemblin, compensatory capacity was 15.02 ÷ 16.32). Levsha, Sibirskiy golozernyy and Progress varieties refer to plasticity varieties (according to A.A. Gryaznov) and the index of environmental plasticity was 1.26 ÷ 1.36; Levsha, Sibirskiy golozernyy and Progress varieties refer to adaptive varieties (according to A.A. Zhivotkov) and their index of adaptivity varied from 126 to 136%. The authors recommend to include glumiferous varieties Sibirskiy golozernyy, Progress, Levsha, Irtysh 23, Orion, Tarskiy 2 (rank sum is 29.0 ÷ 40.0) and huskless variety Levsha (rank sum is 47.0) into the selection and breeding programs and cultivating them in Western Siberia.   

Pollution of water basins caused by the elements of mineral fertilizers removed from the soil by precipitation and floodwaters is a relevant environmental problem. In this regard, the aim is to minimize application of fertilizers and their propping in the soil. Application of NPK fertilizers in the mixture with natural organic fertilizer sapropel is seen as one of the possible approaches and solutions. This component of organomineral mixtures is extracted from the lakes in Novosibirsk Region; its reserves are estimated as 600 million tons. The paper highlights that sapropel contains chelate agents that take the molecules of fertilizers and keep them from moisture removal, and at the same time do not prevent their assimilation by plants. The researchers outlined a significant effect of sapropel on the productivity of spring wheat when it is applied on grey forest soils. When presowing application of sapropel dosed as 4 and 8 kg/m2, the researchers observed significant increase of crops individual productivity in comparison with that in the control group, and gradual growth caused by higher dose applied. The paper highlights the experimental results of the impact caused by sapropel when growing three forms of corn. These are C4-photosynthetic crops, with much more intensive assimilation of CO2 per unit of time in comparison with wheat, and therefore with a more active consuming of mineral elements from the soil. The experiments revealed genetic polymorphism of the studied samples of corn in response to sapropel applied according to quantitative parameters.

The results of long-term agrochemical experiments highlight quantitative and qualitative changes in the humus status of chestnut soil when applying organic and mineral fertilizers. When fertilizers were not applied, the initial humus concentration in the soil was reduced and on average reached its minimum level of 0.94 ± 0.03% in the 48th year of research. During the whole period in the unfertilized variant, the soil lost 28.3% of the initial amount of humus, or 11.0 t/ha, with an average annual loss of 228 kg. Kinetic parameters of humus reducing in the soil of the control variant reached k = 0.008 year -1 in rapid manifestation. When mineral fertilizers were applied, the humus concentration was higher than in the control variant and reached 1.17 ± 0.05% by the last date of determination. The reduction rate in the variant of applying mineral fertilizer was k = 0.003 year -1. The average annual inflow of root and stubble residues when applying mineral fertilizes compensated humus losses and stabilized its concentration after 30 years of research. Reducing of humus reserves in the soil revealed in a corresponding reduction of annual losses, which reached 131 kg/ha in the first 16 years, with further decrease of 107 kg/ha in 14 years, followed by their absence and slight decrease in the last 7 years - 41 kg/ha. Deficient and positive balance of humus was provided by the variant with manure application. The humus concnetration in the soil for 48 years of applying fertilizers reached 1.50 ± 0.04% and significantly exceeded the initial concentration. On average, during the research period the soil multiplied its reserves on 5.6 t/ha with an average annual growth rate of 117 kg/ha. Kinetics of humus concentration increase in soil in the variant with manure application had a growth rate constant k = 0.002 per year. Ranking of positive quantitative (S gen, %) and qualitative (HC: FC) changes of humus in soil according to estimation variants in dynamics of perennial series occurs in a row: no fertilizers (0.56 % and 0.75) → complete fertilization NPK (0.69 % and 0.79) → manure (0.86 % and 0.92).

Research on hyperhaline lakes began in 1977. Commercial interest to Artemia (at the stage of cysts) as a starting feed for hydrobionts served as the basis for monitoring of hyper haline lakes in the Altai Territory. Kulundinskoe lake is recognized as one of the largest basins in the Kulundinskaya plain of Ob-Irtyshskoye interfluve with population of fairy shrimp Artemia Leach, 1819. Comprehensive hydrobiological surveys in the growing season (April-October) 2018 show the dynamics of phytoplankton, zooplankton and stages of Artemia salina development in Kulundinskoe Lake . The researchers analyzed the impact of temperature regime and water salinity in the vegetation period of 2018 on Artemia salina status and productivity of the basin. Water mineralization varied within 83.4 - 93.0 g/l, which indicates lower salts concentration in the leach compared to the average many-year value which is explained by the change of water regressive stage to transgressive one. Monitoring of hyperhaline basin in the vegetation period of 2018 showed a depressive status of Artemia salina development due to demineralization of the basin. At the beginning of vegetation period, the temperature regime was unfavorable for Artemia salina development in the lake. That resulted in low numbers of copepods. Due to depressive development of Artemia salina in Kulundinskoye Lake in the vegetation period of 2018, the basin is seen to have lost its economic relevance.

The paper highlights the results of the system analysis carried out in order to explore the primary characteristics of native seed from Holstein servicing bulls of Black-and-White breed aged 6-7 years. The analysis was carried out to rank ejaculates by the total number of spermatozoa. The experiment was carried out at OAO “Moskovskoye on pedigree activities” in 2011 - 2013. The researchers tested 5964 ejaculates, obtained from 17 bulls during three years, in relation to the season. The researchers explored the number of ejaculates (n), defect of the native sperm when taking (%), and the seasonal dynamics of the total number of sperm cells in the ejaculate. The keeping technology, feeding and maintenance, sperm collection, its assessment and cryopreservation was carried out in accordance with the requirements of the “National technology of producing and application of servicing bulls’ sperm “. Ejaculates were distributed into three groups according to the total number of spermatozoa: - up to 3 billion; - 3.1 - 5.0 billion; - 5.1 billion and more. The researchers found out that the number of ejaculates of good quality selected for cryopreservation in the mature reproductive aged bulls (6 - 7 years) is 76% on average with a variability of 73-82%, depending on the season and year of maintenance. The highest number of the ejaculates of good quality was obtained in autumn and it was 82.3%, which is 7-10% higher than that in other seasons (p<0.001). The analysis of ejaculates over a three-year period showed that the first group contained the main mass of ejaculates, where the number of spermatozoa was up to 3 billion. This parameter varied within 40-48% from season to season; the researchers observed the ejaculates that have total number of spermatozoa equal to 3.1 - 5.0 billion were 28.5-33.4%, the ejaculates that have the highest number of spermatozoa (5.1 billion and more) were 23-29%.

The authors see energy components extremely relevant for animals and their health. Due to this fact, diets with energy components that satisfy pets with nutrients at different stages of growth is significant issue in veterinary nutrition. This could be achieved when applying diets that contain balanced necessary components. The research aimed at exploring the impact caused by ready diets on the growth and development of dogs that differ in age and breed is fragmentary one. The authors make a case about necessity for detailed investigation the growth parameters of animals when feeding them with this kind of diets in the low temperatures of Western Siberia. The impact of the finished ration on puppy growth and development was investigated in one of the s animal shelters in Novosibirsk in 2016. The main food used in the experiment for feeding puppies was YUMMI PREMIUM; the feed contains proteins - 26%, fats - 10, fiber - 7, ashes - 7, moisture - no more than 10, calcium – 1 and phosphorus - 0,8%. The authors observed that 50% are meat ingredients (beef, chicken, by-products), 35 are cereals (wheat, buckwheat), vegetable oils, dried milk and 5% are vegetables, chicken fat, mannanoligosaccharides (MOS), Shidiger yucca extract and sea kelp extract. The following food additives are included in the feed: Vitamin A (17000 IU/ kg), vitamin D3 (1700 IU/kg), vitamin E (200 mg/kg), B1 (15 mg/kg), B2 (20 mg/kg), B6, B12 (100 mg/kg), K3, niacin (85 mg/kg), pantothenic acid (50 mg/kg), biotin (950 µg/kg), iron, zinc, manganese, copper (20 mg/kg), iodine (2 mg/kg) and selenium (0.25 mg/kg). The food of the experiment was eaten by puppies with great pleasure. This experiment has shown that YUMMI PREMIUM for puppies increase body weight and average daily growth rates, as well as improve the main parameters of dogs development.

Lifetime diagnostics of animal brucellosis is mainly based on serological reactions as SAT, RBPT and CFT. The tests determine antibodies by means of antigen produced from Brucella S-cells that mainly contain lipopolysaccharides (LPS). The LPS may cause cross-reactions with other clinically significant gram-negative bacteria; this leads to false-positive results. Due to this fact, the researchers involved in improving. The paper highlights the research results on antigenicity of 5 recombinant Brucella proteins (rOMP19, rOMP25, rOMP31, rBP26 and rSOD) and soluble protein preparations (CSP) of B. abortus and/or B. melitensis by indirect ELISA using cattle and sheep serum samples positive for brucellosis by classical serological tests. CSP appeared to be the most antigenic among the protein specimens; it determined antibodies in 94.8% of the cattle and 69% of sheep. Antibodies which were specific to rOMP19, rOMP25 and rOMP31 were detected in 39%; 50.6 and 76.6% of antibody-positive cows. Periplasmic proteins (rBP26 and rSOD) were observed as less antigenic than outer membrane proteins and revealed anti-Brucella antibodies in 29.9 and 14.3% of the cattle. Recombinant proteins were not detected by antibodies of sheep positive for brucellosis. Antibodies to recombinant proteins by i-ELISA were detected in the small number of the cattle kept at brucellosis free farm (from 2.1 to 12.5%). The results obtained outline the necessity to carry out experimental infection of animals in order to assess properly the capacities of recombinant proteins when diagnosing brucellosis.

The paper summarizes the results of additional information resources of multidimensional statistical analysis resources on the basis of research carried out by the authors. For the large white breed the authors made the equations of multiple regression for the initial forecast of some lifetime parameters of sows, taking into account the obtained reliable values of correlation coefficients on the basis of development at the beginning of animal application. The obtained parameters of phenotypic correlations highlight the possibility of using dairy capacity and number of cows in the herd when weaning to predict the weight of the piglets’ nest aged 2 months within the limits of the corresponding (1, 3, 6) sows’ farrows. The results of using regression equation show that the next farrowing or lifetime productivity of a group of pigs can be estimated by means of several indicators of any farrowing. The article outlines that selection index inheritance coefficient calculated by the method of dispersion analysis of half-sibs according to male (fathers) was 0.41±0.04 (P<0.001). This confirms complex improvement of the the reproductive properties of herd sows by means of the index. This is proved by by the results of ranking the parameters of the investigated features after modeling of 50% of the selection. Similar modeling of dealing with the results related to assessment of fattening and meat qualities of young Kemerovo breed outlines a tendency, which is common for both reproductive qualities of sows and parameters of control fattening. When selecting according to one single feature, the authors observed the highest (lowest) parameters in comparison with the analyzed characteristics that were not subject to selection. However, the index selection prevailed in terms of the set of features.

The authors explored the results of statistical and morphological research on the liver and lungs of the cattle and specified distribution and liver and lung attack caused by larval echinococcosis in respect to gender and age of animals in Talas, Issyk-Kul, Naryn and Chui regions of the Kyrgyz Republic. The degree of cattle suffering from echinococcosis in northern parts of the Kyrgyz Republic averages 6%. The cattle in Issyk-Kul, Naryn and Chui regions suffers from larval echinococcosis more (8-8,8%) than the cattle in Talass region (2,3%). Echinococcosis affects cows and bulls aged 1 year and older. The liver and lungs are the main organs affected simultaneously, as well as further liver and lungs affection separately. The degree of liver invasion is 2 times and even higher than in the lungs. Macroscopic changes in the lungs and liver have diagnostic value. The final diagnosis is based on histological test and specific structures of the hydatid cyst in the histocut.

The authors explored the situation in vegetation on hay harvesting areas and pastures in the mid mountain areas of Central and South-Eastern Altai in 2016-2017. The authors investigated 22 communities of various types of meadows and steppes; 8 cenoses were investigated twice. The vegetation of forage lands indicates moderate or high grazing of animals. 2016 was a wet year and it led to pasture degradation and progressive succession. 2017 was a dry year and grazing led to acute pasture degradation and reducing of surface phytomass in 2.2 – 3.6 times in comparison with the previous year. At the same time, the part of mortmass in the stand grass increases. The researchers investigated the perennial leguminous grasses of Astragalus austriacus, A. austrosibiricum, A. tibetanus, Hedysarum gmelinii, H. gmelinii subsp. setigerum and Oxytropis argentata and found out them to be preserved in grasslands and restored in many cenopopulations when grazing is reduced or stopped.

The paper shows the impact of ultra-low concentrations of medical specimens on neutrophil opsonocytophagic reaction (ORR) parameters, such as phagocytic activity of neutrophils (PA), phagocytic number (PN), phagocytic index (PI), in vitro. The researchers observed the highest phagocytic activity in the control group 3 (placebo-control) in Creosotum C30 and, Ovarinine (P<0.01). When exploring the qualitative parameters of neutrophil opsonophagocytic reaction – phagocytic number – the authors observed the highest index in Ovarinine (26.79±5.13 m.t.), Secale cornutum C6 (16.57±0.46 m.t.) and Thuja D3 (15.58±1.30 m.t.). When estimating the phagocytic index in opsonophagocytic reaction, the inhibitory effect of saline solution (1.64±0.07 m.t.) was observed in relation to the placebo-control group No. 3 (2.42±0.21 m.t.) on 32.23%. The regularities revealed show that the lowest indexes of cellular immunity (FA, FF, FI) are established in Platinum C6; FF and FI - in Lilium tigrinum C12. The highest parameters of opsonocytophagic reaction were observed in the complex specimen Ovarinine, which includes Apis mellifelica C12, Pulsatilla pratensis C30, Sulfur C200, Sepia C6, Creazotum C30, Lachesis C12. Ovarinine is characterized by 9.65% increase in phagocytic activity, 997.95% in phagocytic number and 1106.76% in phagocytic index in comparison with the control group 1 with lactose.

The research explores the relation between productivity of binary grass mixture and mineral nutrition. The hypothesis assumes that nutrient value of the same forage depends on mineral nutrition of forage grasses. According to purpose of research, the authors specified the task which is seen to analyze the impact of mineral fertilizers on productivity and nutrition of forage mass of binary grass mixture sown under the cover. The object of the research is a binary grass mixture based on alfalfa (Medicago + sainfoin). Sowing was carried out under the barley and Sinapis cover on two mineral backgrounds: control (without fertilizers) and fertilized. Fertilizers were applied the day before sowing. Crop yield and the quality of harvested mass were assessed in the appropriate period which was the stage of Medicago budding. Two-year industrial and economic experiment highlighted necessity and efficiency of sowing Medicago binar grass mixtures under Sinapis cover with 25% lower sowing rate and apply mineral fertilizers dosed as N23P60K60 in the forest-steppe of Novosibirsk region. This application contributed to the highest output of metabolic energy in dry matter (37 GJ/ha). The authors observed a positive tendency on harvesting from 1 ha of fodder units (2.14 tons), digestible protein (0.35 tons), as well as the yield of dry (3.58 tons) and green mass (13.7 tons). In this variant the authors observed the highest output of metabolic energy in dry matter (37 GJ/ha). There was a positive tendency to harvest fodder units (2.14 tons), digestible protein (0.35 tons) pro 1 ha and get dry (3.58 tons) and green mass (13.7 tons).

Scientific and industrial experiment was carried out at the poultry farming on Ross308 broilers. The authors explored various schemes of application of propolis tincture for pre venting respiratory infection of poultry of bacterial etiology. The researchers arranged a control group and two experimental groups according to the principle of analogues. The groups were placed in separate isolated poultry houses. Chickens of the control group were fed with antibiotic Tilmipool (0.3 ml/l of water) aged 1-3; 14-16; 25-27 days; their poultry house was sprayed with Ecocide C (0.5%, 1 l/100 m3, exposure 60 min) on 1, 7, 14, 21-22, 28-29, 35-36 days of their life. In the 1st group the antibiotic was applied as it was in the control group; aerosol treatment was conducted with propolis tincture (dilution 1:20, 0.5 l/100 m3, exposure 60 min) during the same age periods. In the 2nd group, propolis tincture (1 ml/l of water) was applied for broilers aged 1-5; 14-18; 25-30 days combined with aerosol treatment of propolis tincture air (dilution 1:20, 0.5 l/ 100 m3, exposure 60 min) for 1, 7, 14, 21-22, 28-29, 35-36 days of their life. The most effective way to prevent respiratory diseases of poultry is seen in application of propolis tincture according to the scheme used in the experiment with the 2nd group. The scheme reduces the number of pathogenic, relatively pathogenic microorganisms in the scrapes from the laryngeal mucous membrane and in the air of the poultry house, activates the immune system and metabolism of poultry, increases livability on 3.0% and live weight on 342.7 g, which eliminates application of antibiotics for preventive measures, providing environmentally safe products of poultry farming.

Newcastle disease is observed and detected on all the continents of the globe, except Australia, and causes great economic losses in poultry production. In the Kyrgyz Republic, Newcastle disease outbreaks were observed in 2015 and 2016. Regardless Newcastle’s pathogen is well explored, there are specific features of disease course and the problem of infection elimination is not solved. The authors explored the appropriate conditions for growing Newcastle’s virus in developing hen embryos. For this the researchers infected chicken embryos with different doses: 10-1 to 10-8 viral propagation was applied for 10-day developing chicken embryos into the allantoid cavity in the volume of 0.1 and 0.2 cm3 (doses of 6,104 to 0.6 50% embryonic infectious dose (EID50 ) per embryo and 1.2-105 to 1.2 50% embryonic infectious dose (EID50 ) per embryo, respectively). The degree of virus accumulation was estimated by means of hemagglutination test according to the titers of hemagglutinins in the allantoic fluid. The titers of hemagglutinins in solutions within 10-1 to 10-8 of virus-containing material shows that at volume of an inoculated virus-containing material of 0,1 cm3 accumulation of hemagglutinins of a Newcastle virus strain occurs at high level and does not essentially differ (Р>0,5); when applied for infecting developing chicken embryos of virus cultivation to 10-6 (a dose in this case ~ 60 50 % an embryonic infectious dose EID50 ).

In the Republic of Tajikistan, linseeds, cotton seeds and rape seeds are used for producing vegetable oil. The authors highlight that until now the impact of cotton, linseed and rapeseed cake, which are seen as high-protein feed additives, on the dairy productivity, composition and properties of milk are not sufficiently investigated for the conditions of the country. The paper highlights the results of two scientific and economic experiments on investigation of the impact caused by cotton, linseed and rapeseed cake on the milk productivity, quality and physical and chemical parameters of milk of highproductive cows of the Black-and-WhiteTajik type breed. The researchers found out that application of various seed cakes when feeding cows in DIM increases the milk yield and improves the quality and physical and chemical parameters of milk. The authors observed experimental cows which received different types of seed cake and the milk their produced and found out that experimental cows exceeded 5.9-12.3 % according to the parameters of milk of natural fat; 4% milk - on 12.0-17.9 %. The cows from experimental groups produced 6.55-11.51 kg milk fat more. Feed costs in energetic feed unit pro 1 kg of 4-% -milk were 7.7-15.2% lower in the experimental groups of cows in comparison with the control group. According to organoleptic and sanitary-hygienic parameters, the milk produced by the cows from experimental groups was of higher quality and fulfilled the requirements of the state standard for purchased milk. Fat concentration increased on0.08-0.19%; total protein - on 0.07-0.15, dry skimmed milk residue - on 0.07-0.16 and dry matter - on 0.15-0.35%. According to the chemical composition, the milk produced by cows, which were fed with linseed cake was the best one. The content of milk sugar, ash, calcium and phosphorus was approximately the same in all the groups. The cost of 1 centner of milk in the experimental groups was 6.3-12.7% lower, and the profitability level of milk production was 9.8-16.3% higher in comparison with the control groups.

The paper explores the impact of monochromatic illumination on replacement chickens of Decalb White cross-breed in the conditions of the Amur region. The chickens were grown from 1 day to 115 days age. The research was conducted in the conditions of the poultry farm “Belogorskaya”. The authors arranged four groups according to the principle of steam-analogues; each group contained 200 chickens and the experiment was carried out in the production unit. The researchers used luminous tube lamp with different colour temperatures for lighting. White lighting was used in the control group, yellow - in the first experimental group, green - in the second experimental group and blue - in the third experimental group. The researchers made blood test of chickens aged 30, 60 and 90 days for morphological and biochemical analysis. The authors carried out control weighing of the poultry at that time. At the end of the experiment, three pullets from each group were slaughtered in order to study the organs. The paper highlights morphological and biochemical blood parameters of replacement chickens at the white light had lower deviations from the physiological rate than at yellow, green and blue lamps. At the beginning of growing under yellow lamps, the concentration of gamma-globulin fraction of protein in the blood serum of chickens was higher than the age rate and the content of albumins was lower. Compared to the control group, the amount of bilirubin (P<0.05) and the activity level of asparagine aminotransferase (P<0.001) were higher under green and blue lamps. In the middle and at the end of the growing period, the number of leukocytes, creatinine, uric acid and asparagine aminotransferase may have increased in chickens under green and blue light. The difference among the results of blood tests of chickens grown under white light was, mostly reliable. When controlling the growth and development of replacement chickens aged 30 and 60 days the authors observed that the average body weight of chickens in white light was 1.5-3.9% higher than in the poultry of experimental groups. The average body weight of the chickens aged 90 days from all the groups was at the same level. The results of the control slaughter of pullets aged 115-days and grown under different lighting conditions were similar, and their sexual maturity was the same.

Trichinellosis is a parasitic disease that affects animals and humans. Trichinella is a causative agent seen as a small round worm invisible to the eye. Trichinellosis affects pets and wild animals. Pigs, horses, dogs and synanthropic rats are seen to suffer from the disease more often among the domestic animals, and bears, wild boars, foxes, badgers and others – among the wild ones. . Human infection takes place when eating meat and meat products as raw dried homemade sausages and ham, kebabs, fried meat and other meat products contaminated with trichinell larvae. Infestation of wild animals is caused by predation or eating of dead animals. Pets are infected by eating slaughter products, food scraps and dead animals (rats). Trichinella are preserved in the animal muscles for some years. Badgers populations are seen as one of trichinosis reserves in Amur region. To determine specific features of trichinella larvae distribution in the lean tissue, the researchers explored the materials of 21 badgers from different areas of Amur region. The researchers used the heads or separate muscle groups for conducting the research. This is explained by remote location of many districts from the regional center. Trichinella larvae were detected by compressor trichinelloscopy and digestion in artificial gastric juice. The analysis showed that the number of trichinell larvae in the same muscle group does not depend on the side of the animal’s body, i.e. their number is almost identical on both the left and right sides. Invasion rate (IR) was defined as ratio of the number of infected animals to the total number of animals explored (in percentage). Invasion intensity (II) was determined by the number of trichinell larvae in 1 g of lean tissue (lye/g). The same method was used to investigate the distribution of trichinella larvae in 15 muscle groups of a badger. Invasion intensity in the infected animals was 14.3%. The largest number of trichinella larvae in a badger is concentrated in the head muscles, and there are no significant differences from the body part. The authors recommend to explore the badger carcasses and muscle sampling mainly from the head.

The authors explored wool production and quality of wool of Aginsk zugalay breed sheep of different age and sex in the pedigree-breeding unit of Lenin agricultural cooperative located in Mogoituiskiy district of Trabs-Baikal region. The results obtained show that untrue unwashed wool of medium-wool breed varied ranged from 1.91±0.06 kg in the case of firsts to 3.92±0.07 kg in the case of producers in output of washed wool untrue 74.13-81.81%. The average thickness of rams’ wool is 32.82±1.27 mkm, ewe - 28.39±0.95, ram hogs - 28.21±0.95 and gimmer - 26.60±1.06 mkm when the strength was 10.89; 10.72; 9.21 and 9.00 cH/tex. Zugalay sheep have high concentration of wool, which varies from 66.61 to 81.72%, and rough beard hair (59.10-72.42 mkm). The highest content of wool was observed in young animals. Untrue wool of all sheep in different age and sex groups is 21.40-23.51 mkm (60-64th quality). The length of woolen fibers of the lower tier was 8.20-8.44, beard hair was 14.74-15.04 cm. The highest amount of fat and sweat was observed in servicing sheep and replacement ram hogs (Р˂0,05). The authors found out high correlation between the body weight and washed wool untrue in the sheep of all age and sex groups, especially in young sheep (0.629 in ram hogs and 0.717 in gimmers), as well as between wool thickness and its length (r=0.273-0.668) and between wool thickness and beard hair (r=0.306-0.687).