X-ray microanalysis methods have significantly expanded the functionality of electron microscopy. X-ray microanalysis is widely applied in activities, which deal with plant physiology. The paper explores the functioning of the stomach apparatus of fodder beans (Vícia fába). The regulation of the stomatal function depends on the potassium concentration in the closing cells. The authors investigated the infestation of wheat leaves with mildew (Erysiphe graminis) (Triticum astivum) and obtained the data that reveal the changes in the elemental composition of tissues in the affected area. This has contributed to find out the role of various elements during the infectious process in case of wheat plants with powdery dew contamination. The paper summarizes the materials on the application of X-ray microanalysis in phytopathology. It stipulates the participation of calcium in the formation of the mechanism of nematode capture in different age colonies of predatory fungi Arthrobotrys oligospores Fres was revealed. The data on tissue barrier in wheat roots, where the transport of both sodium and chlorine salting elements and potassium and calcium macro elements is controlled, are obtained. The authors show that transporting of these elements is regulated by active mechanisms in the endoderm cells. The article demonstrates the results of X-ray microanalysis aimed at determining the content of elements of magnesium, phosphorus, sulfur, potassium and calcium in soybean leaves affected by various diseases. The authors observed the changes in potassium, calcium, magnesium and phosphorus concentration in leaves with bacterial burn, ascochytosis and alternative. They argue the participation of elements in formation of plant resistance to disease and the role of these elements in the physiology of the infectious process.

The development of a variety model suitable for specific soil and climatic conditions is currently an urgent matter in the plants selection. The combination of valuable genotypes of a plant, which is based on hybridization, free pollination, mutagenesis, allows researchers to derive new perspective samples. The selection of the variety model parameters enables the researchers to create cultivated plant varieties that have appropriate characteristics of a particular cultivation zone on an efficient basis. But it is necessary to understand that the model is hypothetical even in case of conducting detailed study of parameters of a new variety based on concrete results of research. First of all, the crop breeder is guided by the imaginary model, which is based on the evaluation of the breeding material and the selection of the variety sample prototype, taking into account the features and properties of the ideal variety model. To a certain extent, the methods of crop breeding and genetic analysis applied in order to assess the breeding value of hybrid combinations are designed to level the inevitable subjectivity noted. When compiling the model of the ideal variety the breeder uses the data on soil and climate conditions of a particular region, the main economic and valuable features of a crop, the results of other researchers and applies intuitive and empirical criteria for evaluating the breeding material. Since 1997 the Chair of Breeding, Genetics and Forestry explored more than 150 breeding samples of different ecological-geographical origin, obtained on the basis of intervarietal hybridization. The samples were studied according to the main economic and valuable features: growing season, growth character, presence of parchment layer and fibers in the seam. Variability of the mentioned features has been estimated by means of correlation links and character of inheritance of separate features.

The paper demonstrates the research results on somatic chromosomal instability that includes the number of chromosomal fragments in the blood cells of the ram descendants, obtained from Romanov servicing rams. The research was carried out on the populations of Romanov sheep at OAO “Vaganovo” in Promyshlennovsky district of the Kemerovo region. The samples were prepared by means of the P. Murkhed method and stained using the Romanovsky-Gimza method. 2580 metaphase blood plates of lambs were studied. The authors analyzed the environmental situation in the breeding area. Studies of soil, fodder, organs and tissues of the animals of different species confirm that there are no heavy metal pollution within the sanitary protection zones in Western Siberia. The authors found out the hereditary regularities of somatic chromosomal instability in the Romanov sheep. The researchers identified the impact of servicing rams genotype on the quantity of fragments of chromosomes in the blood cells of the offspring. The differences in phenotypic variability were revealed in the offspring of different ram-males. The paper shows that the number of chromosome fragments in the sons of some fathers was 3.2 times higher than in the descendants of other servicing rams. Due to the lack of the data on somatic chromosomal instability, the number of chromosomal fragments in the cells of Romanov sheep in Siberia, the data obtained can be accepted as a physiological standard, as well as used to characterize animal interior.

The paper explores the urgency of increasing efficiency of breeding Red-and-White dairy cattle that take the main part of the population in the Krasnoyarsk region (69.7%), the immunogenetic characteristics of animals that belong to Montwick Chiftein and Pabst Hoverner (n=52) lines. The research on the blood groups of cows were conducted in the laboratory of immunogenetic analysis of Krasnoyarskagroplem. The authors revealed the differences in the incidence of specific antigens in the cows of two lines. The article found out that antigens A’2, B2, E’3, O3, and T2 (P>0.95-0.99) were found to be more frequent in Montwick Chiftein line cows, and antigen P2 (P>0.95) wasfound in Pabst Governer line cows. It was also revealed that the most rare antigens in the Montwick Chifthein line are antigens A’, A’1, O2, O4, Y1, R1, X1, M and E’2, and antigens B’’, O4, U and U’’in the Pabst Governer line. Interestingly, the authors identified the antigens that were not present in the animals of both lines and in each line separately. The average index of antigen incidence in the cows of each line showed a relatively low value. Due to this the authors made a conclusion about a high degree of heterozygosity of the studied individuals. The index of genetic similarity among the animals of two lines demonstrates a high value of 0.863. The polymorphism of the antigens in the EAF-V locus was studied and a significant incidence predominance in cows of the F allele over the V allele was found out. The impact of genotype according to the EAF-V locus on dairy productivity and live weight of the cows was determined: in the Montwick Chifthein line, cows with the FF genotype (5986,60172,41 kg) had a higher yield, in the Pabst Governer line - with the FV genotype (5914,20208,19 kg). Animals of both lines that had genotype V/V had higher live weight parmeters than animals with genotypes F/V and F/F. The comparative analysis of the obtained results was carried out with the results of other Russian authors who conducted similar studies.

The authors argue the impact of environmental stress factors on physiological status of Simmental cattle of local selection, adapted to conditions of breeding in Arctic zone, characterized by weak forage base and unfavorable extreme natural conditions. They found out the total number of red blood cells and the level of hemoglobulin in the investigated animals in the peripheral blood is 14.55% lower than the standard values on the average. The granulocyte concentration in the leukocyte formula exceeds the standard by 1.2%. The level of total protein in the blood serum is significantly higher than the standard one: in calves - by 38.01, in cows - by 17% (P0.001). In peripheral blood, dysproteinemia is observed in the animals. The impact of environmental stress factors on neutrophil opsonophagocytic reaction (ORP) parameters, such as phagocytic activity of neutrophils (FA), phagocytic number (FN), phagocytic index (PI), in vitro, has been determined. The greatest growth of phagocytic activity was observed in the winter stable period, which exceeded the physiological standard by 5.99%. The FA is relatively high in other seasons and corresponds to the upper limit values of the multiyear average. When studying the qualitative characteristics of microphages - phagocytic number - it was revealed that the maximum values are observed in cows in winter-spring period (10,3±1,6 and 10,0±0,7 m.t.), and the minimum - in summer (7,9±0,9 m.t.). When estimating the phagocytic index in the opsonophagocytic reaction, the authors revealed the profound growth of PI values in winter (8.1±1.4 m.t.), which is 34.56% higher than in summer. The data obtained confirm the severity of the impact of environmental conditions, in particular, seasonal changes that play specific role in the adaptive response and in the preservation of the overall organism resistance.

The authors found out that some districts of the Tomsk region are unfavourable in respect to bovine anaplasmosis. In enzootic areas with bovine anaplasmosis the bovine leukemia was observed in susceptible animals. The research focuses on hematological and immunological changes of the blood of cows infected with leukemia during the acute anaplasmosis. That was found out in spring while intense activity of ixodids (the end of April). The researchers arranged two groups of Black-and-White cows aged 2.5-3 years infected with leukemia virus. The 1st group was infected with acute anaplasmosis cow leukemia virus, the 2nd group (control) was infected with anaplasm-free cow leukemia virus. The changes in the hematological pattern of cows infected with leucosis in case of complications with acute anaplasmosis consist in eosinophilia, neutropenia, lymphocytosis and monocytopenia. The changes in the immune system of cows were less noticeable and characterized by a moderate increase in IgG and high titers of antibodies to leukemia virus. The researchers assume that there is a significant sensitization of the humoral component of the immune system of cows infected with leucosis in case of complications with acute anaplasmosis. The noticeable lymphocytosis in the certain cows infected with leukemia virus in case of complications with acute anaplasmosis should be taken into account when making a diagnosis for cattle leukemia. This occurence in the hematological picture of blood can be characterized as a temporary leukemia reaction, causing a false positive result of hematological test on leukemia.

The paper shows the results of biochemical analysis of the protein status of Kemerovo pigs. The research was carried out at OOO Chistogorskiy in Kuzbass. The blood samples from pigs were taken by means of the standard methods and analyzed in the laboratory of veterinary genetics and biochemistry of Novosibirsk State Agrarian University. The researchers used standard tools for biochemical tests on the biochemical semi-automatic analyzer Photometer 5010 V5+. The concentration of some protein metabolic parameters was characterized by a standard distribution: total protein (p-value=0.85), albumin (p-value=0.06), and globulin (p-value=0.65). The average values of total protein and albumins varied in standard intervals, except for the level of globulins, which was significantly lower than the reference values set for pigs. It was seen as one that had the greatest individual fluctuations (44%). The urea concentration was slightly lower than the physiological standard and was characterized by a rather high level of individual variability (50%). The researchers found that uric acid concentration was 3.2 times lower than the standard one in the investigated pigs. The authors observed the interbred differences in the content of total protein and albumin comparing the Kemerovo, Chistogorsk and early maturing pig breeds (CM-1). The correlation analysis has shown that there is a high positive correlation between total protein and globulins (r=0.88). The average positive relation between urea and uric acid was (r=0.58). The obtained data on protein metabolism is one of the basic characteristics of the Kemerovo pigs interior.

The authors investigated the effect of probiotic Vetom 1 based on Bacillus subtilis strain DSM 32424 with concentration of active component 106 CFU/g on cumulative indices of Japanese quail egg-laying. The specimen was applied in doses of 50, 75 and 100 mg/kg of the live weight of quails. The probiotic Vetom 1 increases the number of ovulating birds. The maximum increase in this indicator was observed when using Vetom 1 dosed as 50 mg/kg of the body weight. The egg mass variability depends completely on the dose. Being used in a dose of 50 mg/kg weight, a higher variability of the egg mass was observed. This pattern has not been recorded in the maximum studied dose. The cumulative function in terms of eggs in unit and mass equivalents is slightly expressed in the initial period when applying Vetom 1. In the final period of specimen applying, the highest accumulation is observed when using Vetom 1 in a dose of 50 mg/kg of mass. When applying Vetom 1 in doses of 75 and 100 mg/kg, a direct correlation between ovulation frequency and egg weight was observed only in the initial period of the specimen applying, and the dose of 50 mg/kg - only for the eighth day of it.

The researchers investigated the effect of new probiotic Vetom 1 based on apathogenic bacilli on the biochemical parameters of blood serum, exactly protein, albumins and urea in lactating cows. The experiment was carried out at OOO “Uchkhoz Tulinskoe”. According to the principle of pair-analogues, the researchers arranged a control group and two experimental groups of cows; each group contained 7 cows. The cows of the 1st experimental group were fed with probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking; They received Vetom 1 every day during 5 days, then in a day during a month. The cows from the 2nd experimental group received probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking, every day during 30 days. The application of the specimen contributed to a decrease in the concentration of total protein in the blood serum within the physiological norm. The aftereffect caused by Vetom 1 when the specimen was applied 5 days every day, then in a day during a month, the authors observed an increase in the concentration of total protein in the blood serum above the physiological standard on the 180 day of application. The similar effect was not observed when Vetom 1 was applied daily. When using Vetom 1, the authors observed a slight increase in the concentration of albumins in the blood serum, both during the period of application and up to 180 days. The specimen contributes to less prominent increase in the concentration of urea in the blood serum in comparison with the control group. Changes in concentrations of albumins and urea occurred within the physiological norm.

The article discussesthe current problem of food quality control, the content ofstrontium-90 and cesium - 137 in the body of wild game birds-wild game inhabiting the territory of the Republic of Sakha (Yakutia). The object for research were carcasses of wild game 3 species: black grouse; partridge; grouse. Radiation safety of wild game as a food product was determined by its compliance with permissible levels of specific activity of radionuclides, in particular 137Cs and 90Sr. These indicators are regulated by TR CU 021/2011 “on food safety”. According to the long-term (2013-2017) results of our studies 137Cs and 90Sr in carcasses of wild game, Northern, Central, Vilyuisk and southern ecological zones of the Republic of Sakha (Yakutia) was not found to exceed the permissible concentration. As a result of our research, the following results were obtained: the content of 137Cs and 90Sr in grouse carcasses: southern - 70.54±0.71 and 26.43±2.54; Central-64.81±0.18 and 20.89±1.95; Vilyuiskaya - 73.12±0.01 and 28.48±1.18. Black grouse does not live in the Northern ecological zone. The results of the study of partridges are as follows: South-69.04±2.95 and 28.17±0.05; Central - 58,69±2,18 19,87 and±0,39; Vilyui - 70,84±1.55 and 27,87±0,04; North - 28,17±0.05 and of 10.83±0,50. In the study of grouse is established: the content of radionuclides in the southern zone - 71,24±1,95; Central was 60.05±0.01 and of 21.11±1,95; Vilyui - 71,11±0.55 and of 11.47±0,32. According to the results of our studies, the data of exceeding the maximum concentration of 137Cs and 90Sr in the carcasses of wild game in all ecological zones of Yakutia were not established.

The authors argue the reindeer’s vascular morphology is not sufficiently investigated. They focus on the distal part of the thoracic and pelvic limbs, which the reindeer steps on the ground and hurts it often, as it leads to necrobacterial lesions in summer. In the postnatal period of the reindeer growth, the most intensive one in the length and diameter of the main arteries is observed in the first year, especially in the first month. During this period, the newborn deer gets into the new conditions of existence and the organs of movement become more and more functional. By the age of 3 - 3.5 years, the growth of the main arteries in length is almost over. The paper explores the features of blood supply of the thoracic limbs of a domestic reindeer, caused by the prominent growth of the second and fifth fingers. The absolute growth of arterial length in newborns and deers aged 0.5 months is 0.6 cm per day. In the following years of life, the length is reduced to 0.03 cm in the reindeers aged 3 y.o. The relative diameter of the arteries to the limb length in the postnatal period decreases from 4.4% in newborns to 3.3% in old deer. The irregularity of changes in the absolute and relative development of the thoracic limb arteries can be explained by different growth of blood vessels, bones, joints and limb muscles.

The article demonstrates the research results on climate conditions of the Republic of Sakha (Yakutia) and grounds of application of precision technologies for breeding Kalmyk beef cattle on the basis of specific natural and climate conditions of Yakutia. The history of animal breeding says that enhancement of local livestock’s producing qualities in different years contributed to the fact that the stud breeds of cattle, generally dairy and beef-dairy types, were imported. Animals of the beef productivity type, known as Kazakh Whitehead cattle, the Hereford, the Kalmyk, and the Calloway breeds, were imported in order to receive crossbred youngsters with improved feeding and beef parameters. In all the time of the development, the cattle breeding in the region had beef-dairy types. The beef breeds were imported in the last decade for targeted purebred breeding and development of the beef industry for production of the beef. Therefore, in 2012, there were imported 200 animals of the Hereford cattle; the Kalmyk breed has been imported since 2013. The experience has shown that naturalizing of purebred animals to the breeding conditions of Yakutia was extremely stressful, and was followed by the diseases, falls, and decreasing of seed stock’s reproductive indicators. Particularly negative impact was observed on the animals of the highly productive Hereford breed, and this caused gradual degeneration of the breed under conditions of Yakutia. Although, specific natural and climate conditions of Yakutia significantly differ from that in other regions with developed beef cattle breeding, the animals of the Kalmyk breed, which was bred on the basis of the native steppe cattle, were relatively well adapted to the harsh conditions of the region. The authors argue the necessity and urgency of adaptive technologies development on specialized beef cattle breeding with respect to the harsh local conditions.

The paper demonstrates the results on the effect of new specimen Vetom 20.76 on concentration of leukocytes in the blood of geese on the basis of the predatory fungus Artusbotus oligospora. In order to achieve the goal of the experiment, one control group and six experimental groups were arranged on the principle of paired analogues. Each group contained 10 geese aged 1 month. The geese from the experimental groups received Vetom 20.76 in different doses in the morning with water once a day: the geese of the 1st experimental group - dose of 0.5 ppm/kg of live weight during 15 days; 2nd experimental group - 1 ppm/kg of live weight during 15 days; 3rd experimental group - 2 ppm/kg of live weight during 15 days, 4th experimental group - 0.5 ppm/kg live weight during 30 days, 5th experimental group - 1 ppm/kg live weight during 30 days and 6th - 2 ppm/kg live weight during 30 days. The geese of control group didn’t receive the specimen. The concentration of leukocytes in the blood of experimental geese increases in the period of specimen application as well as in the period of its aftereffect. If Vetom 20.76 is prescribed for 15 days, the effect of leukopoiesis stimulation finishes on the 30th day. If the specimen is applied during 30 days, the leukocytes in the blood continue to increase up to the 60th day. This long-term application of Vetom 20.76 dosed 0.5ppm/kg increases leucocytes within the physiological norm. Application of higher doses (1 and 2 ppm/kg) the leukocyte concentration conforms to the physiological norm