The research was carried out to study the influence of biologization and chemicalization factors on the phytosanitary state of barley in the chain of the six-field fodder crop rotation of the second rotation in the forest-steppe of Western Siberia. The biologization factor was considered sowing peas to barley. The chemicalization factor was considered as the introduction of mineral fertilizers according to the results of agrochemical analysis at a dose of N₆₀P₂₀. It was found that the highest development of root rot was found in the variant with sowing barley without fertilizers, where the disease development index reached an average of 14.4% for the plant. The lowest one is in the variant “barley + peas + fertilizers’ (4.6%). In the variants “barley + peas’ and “barley + fertilizer”, the intensity of the disease was approximately the same and within the limits of the severity threshold – from 6.6 to 6.9 %. Similar data were obtained for the prevalence of the disease. Among the analyzed organs, the primary roots had the greatest damage, which was due to significant infection with Bipolaris sorokiniana Sacc. Damage by leaf-stem infections was almost 2 times lower in the variant with the use of the legume component and the application of fertilizers (16.8%) versus the control variant (31.8%). It was found that the correlation coefficient of the root rot development index on average for a plant with productive tillering was r = –0.63, and with plant height r = –0.99. Confirmation of the harmfulness of root rot is a close negative correlation between the yield and the indicators of the development of the disease on average for the plant: r = –0.74. It was found that the most favorable phytosanitary situation developed in the variant of sowing barley with the use of fertilizers and a legume component, which is associated with the healing effect of fertilizers and legumes, which is reflected, on the one hand, in increasing the endurance of the plant itself, and on the other, in an inhibitory effect on causative agents of the disease.

The ecological and biological characteristics of woody plants growing in the most popular squares of the Novosibirsk city (Teatralny, Pervomaisky, and Slavy square) have been investigated. 73 species of woody plants have been recorded. 13 of them are common to three landscape objects. Ornamental garden forms of woody plants are found separately (13 intraspecific forms, hybrids and varieties of woody plants). Some species of molded plants that are not suitable for landscaping public urban space due to their low winter hardiness were recorded. The greatest species diversity is distinguished by the green spaces of the Slavy square. The correlation between the place of growth and the vital state of plants has not been found. Plants on the objects are in satisfactory vital condition. Species in an unsatisfactory condition are found singly. Shrubs (49% of the total) and trees of the first and second sizes (32%) predominate in terms of life forms. The plantations contain species of natural flora of the Novosibirsk region and introduced plants from other geographic regions (27 and 73%, respectively). Among the introduced plants, species with a wide Eurasian type of area prevail. Analysis of the history of the creation and reconstruction of landscape objects showed that the assortment of plants on the territory of Pervomaisky Square has changed to a greater extent, the species composition of plants in the Teatralny Square has changed insignificantly. To form a better spatial structure of landscape objects, we recommend to increase the species and form diversity of plantings by introduction of plants of various life forms and species that have passed introductory tests in local climatic conditions. This will provide the creation of landscapes and landscape compositions of various expressiveness and to form a comfortable environment for the rest of the citizens.

The aim of the work is to study the effectiveness of the use of trace elements on potato varieties of different ripeness groups in the northern forest-steppe Ob in the Novosibirsk region. Experimental data were obtained on leached black soil of Novosibirsk region in 2017–2019. The generally accepted technology of potato cultivation was used for the experiments. Since autumn, fertilizers at a dose of P₉₀K₉₀ were applied as a general background for potatoes. Nitrogen fertilizers (60 kg / ha) were applied in the spring for pre-sowing cultivation. Foliar dressing with microelements (Cu, B, Zn) was used at the beginning of the budding phase of potato plants: copper – 20, boron – 45, zinc – 50 g of active ingredient per 1 ha with a working fluid consumption of 300 l/ha. Trace elements increased the growth and development rates of potato varieties of three groups of ripeness. In varieties Liubava (early), Svitanok Kievsky (mid-early), Tuleevsky (mid-season), the use of trace elements contributed to an increase in the development indicators of the photosynthetic apparatus (maximum and average leaf area and plant productivity) by an average of 21%, especially against the background of boron and zinc. Foliar dressing of potatoes increased the yield parameters by an average of 24 % relative to the control (water). The increase in the marketability of tubers reached 12 %. A greater effect was achieved against the background of boron use: a significant increase in yield, dry matter, starch, vitamin C. In variants with microelements, the susceptibility of potatoes by rhizoctonysis decreased.

The influence of hydrothermal conditions on the duration of phaseolus vulgaris L. phenophases of the grain direction at different sowing dates in the forest-steppe conditions of the Ob region was studied. The research was carried out on the experimental field of the training and production farm «Sad Michurintsev» at the Novosibirsk State Agrarian University. The objects of the study were the Rubin variety and the promising variety Krasno-pestriy. The structure of the growing season was studied and the samples were assessed by the duration of individual phenological phases. The influence of hydrothermal conditions on the change in the duration of the «sowing-emergence» interphase period was established at different sowing periods (up to 8 days) and the «seedlings-flowering» and «flowering-biological ripeness’ periods (up to 4 days). A one-way analysis of variance was carried out with a confidence interval of 5%. The optimal average sum of active temperatures for the passage of the main phenological phases «sowing-germination», «seedling-flowering» and «flowering-biological ripeness’ has been established: in Rubin it is 244, 518 and 709оС, and Krasno-pestriy is 241, 564 and 760оС, respectively. A relationship between the duration of the growing season and the average daily temperature (–0.90), the sum of temperatures (0.96), the duration of the growing season and the sum of precipitation (0.90) was revealed. The difference in precipitation of growing seasons at different sowing dates is insignificant and ranges from 120 to 131 mm. According to the results of the study, the recommended sowing time for the main groups of beans ripeness in the foreststeppe conditions of the Ob region is the second half of May.

The aim of the study was to determine the influence of plant species and year conditions on the biological diversity and number of insects – inhabitants of the crown layer of woody plants of the Rosaceae family in the conditions of the northern forest-steppe of the Ob region. The research was carried out in 2017–2018. During the flowering period of woody entomophilous plants, insects were collected by mowing with an entomological net in the crowns and undercrown space (25 strokes in four repetitions). In the crown of woody introduced plants Pyrus ussuriensis (Ussuri pear), Prunus maackii (Maak plum), Amelanchier alnifolia (alder irga), Spiraea betulifolia (spiraea birch leaf), Physocarpus opulifolius growing on the territory of arboretum of RAS in the northern forest-steppe of the Ob region, a total of 2597 insect specimens from 7 orders and more than 30 families were found. The largest number of insects belonged to the order Diptera (49.4 %). Representatives of the orders Thysanoptera (23.7%) and Hymenoptera (11.4%) made a significant contribution to the formation of the entomofauna. The entomofauna of different species of woody plants from the Rosaceae family differed in the taxonomic groups of insects and their numbers. A high degree of enomofauna similarity (Jaccard coefficient is 0.75) was found between Amelanchier alnifolia and Pyrus ussuriensis with similar flowering periods. A low degree of similarity was found between Amelanchier alnifolia and Spiraea betulifolia (0.32) and between Pyrus ussuriensis and Physocarpus opulifolius (0.33). The species of the plant (38.1 and 26.1%, respectively) had the greatest influence on the biological diversity and the number of entomocomplexes, which indicates the adaptation of insects to a woody plant of the Rosaceae family. The conditions of the year significantly (by 9.8%) influenced the biological diversity of insects during the flowering period.

Many scientists have established the multifunctional effect of bacteria of the genus Bacillus on various plants. It is necessary to use natural growth regulators to obtain high yields of tomato. The paper presents the results of studies on the effect of a mixture of strains of the genus Bacillus bacteria on the growing processes and productivity of tomato. In 2019–2020 under the conditions of the Novosibirsk region, in small-plot experiments, it was found that a mixture of bacterial strains of the genus Bacillus (B. amyloliquefaciens VKPM B-10642, B. amyloliquefaciens VKPM B-10643, B. subtilis VKPM B-10641) has a growth-stimulating effect on a tomato Spok variety. Their use contributed to an increase in the vegetative mass of plants. The treated plants, in comparison with the control sowing, were taller: the length of the aboveground part increased 1.2–1.4 times on average over 2 years. When using a mixture of strains of the Bacillus genus, the number of brushes, flowers and tomatoes increased. A statistically significant difference in the number of flowers and fruit was obtained when accounting for the first and second ten days of July. The number of fruit increased significantly in the second decade of July by 1.7 times. It was found that the pre-sowing treatment of tomato seeds with a mixture of strains not only contributed to an increase in growth rates, but also positively influenced the formation of the yield. Under the influence of the microbiological complex, the average weight of fruit increased by 1.2–1.4 times. The yield increase on average over 2 years was 4.5 kg/ m ². In this regard, it is possible to recommend the use of a mixture of strains of bacteria of the genus Bacillus at a concentration of 1×10⁶CFU/ ml for pre-sowing treatment of seeds as a growth stimulator and increase the productivity of tomato Spok variety.

In the non-black soil zone of Russia on sod-podzolic and floodplain soils, the adaptive properties of forage crops of the bluegrass family were assessed in terms of ecological stability and plasticity and the effect of mineral fertilizers on them according to the yield criterion. The analysis of changes in the yield of forage crops was carried out from 2009 to 2015. It was characterized by different weather conditions. As a result of the research, it was found that the environmental index varied over the years from –6.36 to 6.08. The highest adaptation coefficient was observed in Sudanese grass (1.37) and millet (1.33). The smallest yield variability was found in the cultivation of awnless brome (10.8%), millet (13.2%) and annual ryegrass (14.6%). The highest stress resistance was found in one-year ryegrass (–6.00) awnless brome (–6.20) and oats (–6.50) – the main forage crops in the study area. The minimum value of the yield range in contrasting conditions was set in millet (20.4) and awnless brome (22.0). The most stable crops in obtaining the harvest were two-source reed, annual ryegrass, Scleranthus, meadow fescue and Sudanese grass. Sudanese grass, millet and oats were the most responsive to changes in cultivation conditions. The most intense (bi = 2.40, and Sd ² = 0.39) fodder crop in the western Briansk region is Sudan grass, while millet and oats are highly responsive in combination with low yield stability. The awnless brome reacts poorly to the improvement of external conditions, but has a fairly high yield stability. The use of mineral fertilizers increases the environmental index, adaptation, variability of the yield of forage crops, increases the gap between the maximum and minimum yield, and also enhances the stability and reduces the responsiveness of crops to environmental changes.

The effectiveness of the fight against helminthiases depends on the knowledge of their epizootology, age and seasonal dynamics. The Amur Region is located in a sharp continental climate zone, which affects the living conditions and health status of farm animals, including the development of various parasitic diseases that negatively affect the productivity of farm animals. The biological cycles of helminths are characterized by seasonal dynamics of development, since most of them take place with the participation of intermediate hosts that are sensitive to changes in climatic conditions (temperature, amount of precipitation), as well as to other factors. The determination of the onset of infection timing, the most unfavorable seasons and the main types of helminths are necessary for the prevention of helminthiasis in each specific region. It has been established that the basis of the population structure of helminthiases in cattle under the conditions of the Amur Region is trongylatoses, strongyloidiasis, neoascariasis and moniesiasis. The first eggs and larvae of Strongylata spp. Found in February – April. The greatest extent of invasion was observed in the winter period (February). Moniezia benedeni, as well as Neoascaris vitulorum, had the highest infection rates in the autumnwinter period. At the same time, in the annual dynamics, the first eggs of helminths in both species were found in February. Cases of detection of other helminths in cattle were first recorded mainly in the spring. Thus, the infection of cattle with helminths in the Amur Region does not depend on the grazing season, which should be taken into account when developing plans for anthelmintic measures in livestock farms.

The seasonal variability of the physicochemical parameters of milk of both ordinary goats and goats producers of a biosimilar human lactoferrin has been established. The average values of the mass fraction of fat, protein, lactose, nonfat milk solids, density index in the second and third quarters were lower than in the first and fourth quarters. At the same time, the difference between the spring-summer and autumn-winter periods in the mass fraction of fat was 25%. In the mass fraction of protein, lactose, nonfat milk solids, density it was 5–7%. Comparative analysis of milk from goats-producers of a biosimilar human lactoferrin of various lactations revealed a number of changes in the physicochemical composition of raw materials in comparison with non-transgenic animals: an increase in the mass fraction of protein by 4–6% (P˂0.05), of lactose by 2–6 (P˂0.05), nonfat milk solids by 1–6, density by 2–5% (P˂0.05), freezing temperature by 5 and a decrease in the mass fraction of fat by 5–8% (P˂0, 05). At the same time, the indicators of active and titratable acidity of milk were similar in all groups of animals. At the same time, the concentration of human lactoferrin in the milk of producers of the second and third lactation had almost identical seasonal changes in the increase and decrease in the synthesis of lactoferrin in the mammary gland during the year: in the first quarter – 2.88 and 2.97 g/l, in the second – 4.76 and 4.63, in the third – 7.44 and 7.55 and in the fourth – 7.97 and 6.72 with an average annual value of 5.84 and 5.72 g/l, respectively.

The characteristic of bulls-producers of red breeds of OAO Barnaulskoe breeding enterprise on the quality of sperm and its resistance to cryopreservation are presented. The breeding enterprise has examined samples of water, soil, feed for the content of heavy metals. In the studied samples, no excess of the LOC was found. The research results were processed on a PC using population statistics methods. Angler bulls produced the largest amount of ejaculates unsuitable for cryopreservation. It is 20.6%. According to this indicator, they surpassed the producers of red Danish and red steppe breeds by 4.1-7.8%. The animals of the latter breed showed the smallest amount (1.7%) of ejaculates rejected after defrost. In Angler bulls, this indicator was 5.95%, in red Danish bulls it was 2.95%, which is 3.52 and 1.75 times higher than in red steppe breeders (P <0.01-0.001). Significant differences were established between individual bulls of the red steppe breed in the number of ejaculates rejected before cryopreservation (2.84-22.29%) and after defrost (0-7.09%). The differences between breeds in the frequency of rejected ejaculates before freezing and after cryopreservation indicate a certain role of heredity in the resistance of animals for these indicators. Spearman’s rank correlation coefficient between these traits was 0.312. It indicates the possibility of preliminary assessment and selection of bulls according to the first indicator. But the final assessment of the bull-producers can be made by the frequency of rejected ejaculates after thawing. We propose to consider the issue of additional accounting in breeding programs for the resistance of the seed of bulls-producers to cryopreservation.

The object of research was the American mink, a predator, a semi-aquatic representative of the mustelidae family. The study was carried out on an adult population of males and females of the 22^nd generation of different behavioral types and genotypes at the Experimental Fur Farm of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences. The total number of studied animals was 547 ones of the Standard dark brown (+/+), Hedlund-white (h/h) and black crystal (CR/ +) genotypes. All animals were kept in the same conditions and received food edlibitum (lat. – full) on a unified ratio once a day in the afternoon. The research results were processed using a standard biometric method. The comparison was carried out according to the Student’s test. The aim of this work was to study the course of molting of the American mink depending on the genotype, behavioral character and gender of animals. At the end of August, a visual assessment of the course of molt along the tail was carried out. Points were assigned individually to each animal (from 0 to 5 points). During the study of the molting process of the American mink, it was found that in male aggressors of the black crystal and headlund genotypes, molting proceeded much more intensively than in animals of the standard genotype (by 1.9 and 1.3 points), and in females of the aggressive type of behavior of the standard genotype it was faster than in females of genotypes headlund and black crystal (by 1.8 and 1.6 points). When comparing gender data in animals of different genotypes and behavioral reactions, the best molt course was found in male aggressors of the Hedlund and Black Crystal genotypes in comparison with females of the same genotypes (by 0.6 and 0.5 points, respectively). Due to domestication and selection, changes occur in the economically useful traits of animals. Research in this area makes it possible to study these processes and apply them in practice. Ignoring the biological characteristics of minks creates a threat to the emergence and development of serious abnormalities in metabolism, which can lead to a decrease in productivity and even death.

Studies on the accumulation of manganese in females and males in the scales and muscle tissue of the Sander lucioperca of the Novosibirsk Reservoir were carried out. Analyzes were taken in a A.M. Nikolaev analytical laboratory of the Institute of Inorganic Chemistry SB RAS. The pike perch were divided by gender. Then 34 samples of scales and muscle tissue were taken from them. The manganese content was determined by inductively coupled plasma atomic emission spectrometry on a Thermo Scientific iCAP-6500 spectrometer. High variability of manganese accumulation in females and males in scales and muscle tissue was revealed. It was found that the concentration of manganese in females scales was 7.7 times higher than in muscle tissue, and in males - 15.7 times. There were no significant differences in manganese content between the genders. The ratio of extreme variants of scales in females and males was 1: 5 and 1: 6, and in muscle tissue - 1: 35 and 1: 30, respectively. The average values of the concentration of manganese in the muscles of the Sander lucioperca of the Novosibirsk reservoir were determined. Differences in the deposition of manganese in muscle tissue in females and males of Sander lucioperca were revealed. The concentration of manganese in the muscles of females was 2 times higher than that of males. The results obtained on the accumulation of manganese in scales and muscle tissue can be used as reference values. The level of metal concentration is characterized by an uneven distribution in the muscles and scales of Sander lucioperca. The research results are suitable for a lifetime assessment of the Sander lucioperca interior. An average positive correlation was found between manganese in females with absolute length and body weight (r = 0.329 and r = 0.311). It was found that the amount of manganese and other heavy metals in the Novosibirsk reservoir is increasing.

The main goal of the work was to determine the attitude of the American mink to feed consumption, depending on the genotype and behavior. The object of research was American mink (Neovison vison) of cell breeding of three different genotypes: Standard dark brown (+/ +), Hedlundwhite (h/ h) and Black crystal (C_R/ +). Groups of animals were initially assessed on aggressive and manual behavior using a special hand catch test method. The duration of the work was 30 days. All animals received a unified ratio according to the recommended norms. Daily feed was weighed before distribution (190 g per 1 head to females and 250 g to males) and the amount of feed consumed a day per group and per 1 head was determined from the remains. The feed consumption was assessed by the indicator of the feed consumed by males and females of different genotypes and behavior. Comparison of the data was carried out using Student t-test. As the temperature factor influences the feed consumption, the feed intake by minks of different genotypes was assessed over seven-day periods. It was found that in the first observation period there was no difference in the amount of feed consumed by females. However, in the second period, aggressive Hedlund females consumed more than females of the black crystal genotype. In the same period, the females of the black crystal genotype showed the lowest feed consumption in comparison with the standard and Hedlund females. In the third period, Hedlund females consumed the largest amount of feed among aggressor females in comparison with the genotypes Standard and Black Crystal. There are no differences in feed consumption by males of different genotypes for the same periods. Over the entire study period, female aggressors of genotypes Standard and Hedlund consumed more feed than females of the tame type of behavior. The tame males of the standard genotype consumed more feed than the aggressive ones. There were no differences in feed consumption by minks of different behavior of the black crystal genotype.

The study of the biological role of chemical elements in farm animals is one of the issues that scientists continue to investigate. A comprehensive study of the gene pool and phenofund of breeds and species of farm animals is being carried out in Siberia. An important issue is the search for vital markers of macronutrient accumulation, including sodium in organs and tissues of animals. The article presents data on the content of sodium in the blood serum of offspring obtained from bulls-producers of the Holstein breed. The experiment was carried out at OAO Vaganovo, Kemerovo region. The sodium concentration was determined by the atomic emission method on an ICP AES IRIS spectrometer at the V. S. Sobolev Analytical Center for Collective Use of the Institute of Geology and Mineralogy SB RAS. In the area of Holstein cattle breeding, there is no excess of LOC for heavy metals in soil, water, feed, organs and tissues of animals. The influence of the genotype of the fathers of the Holstein breed on the sodium content in the blood serum of sons was established. The sodium concentration in Fabio’s offspring was 1.9 times higher (240.4 mg/ l) than in Brio’s sons (P ˂ 0.001). By the level of sodium in the serum of offspring, bulls-producers were arranged in the following order: Brio ˂ Bonaire ˂ Malstrom ˂ Fabio in a proportion of 1: 1.3: 1.5: 1.9. The phenotypic variability of this element between the offspring of different producers differed by 3 times or more. Interbreed differentiation, the influence of the genotype of producers, differences between lines and families sign the hereditary determination of resistance and susceptibility to the accumulation of macro- and microelements in organs and tissues of animals.

Intensive dairy farming in modern conditions is impossible without a solid forage base and the use of high-quality forage resources. A high level of cow productivity presupposes adequate nutrition for the animal at all physiological periods of its life. Due to the fact that cows consume relatively little feed during the first period of lactation, the primary task of livestock breeders is to provide the body of highly productive animals with the necessary amount of energy, protein and other nutrients. The article presents the data of a scientific experiment on the use of non-degradable protein in the rumen PassPro Balance in diets for fresh cows. The feed additive under study contributes to an increase in the level of dry matter consumption by animals. The average daily milk yield of the experimental group, which received the studied supplement, significantly increased by 12.0% (P <0.05) in comparison with the control group. There was also established a tendency to an increase in the fat content in the milk of cows from the experimental group. According to the biochemical analysis of the blood serum of cows, the level of albumin in all animals was within the physiological norm. The content of globulins was below the norm in animals of all groups without significantly significant differences. The activity of the AST and ALT enzymes between the groups did not differ significantly. However, it should be noted that in both groups these indicators were within the normal range. The level of alkaline phosphatase tended to increase in the experimental group by 20.8% in comparison with the control group. Feeding PassPro Supplements Balance in the diets for the experimental group made it possible to receive 2565,00 rubles of additional profit, while the level of profitability of milk production increased by 2.02% relative to control.