ЗЕМЛЕДЕЛИЕ, АГРОХИМИЯ, ЗАЩИТА РАСТЕНИЙ
The work is devoted to the study of bean crop cultivar response to biological preparations at pre-sowing seeds treatment (for productivity elements). The experimental part of the work was conducted at Omsk State Agrarian University after P. A. Stolypin that is located in West Siberia southern forest-steppe during the years 2010–2012. Seven bean cultivars were used as objects for the study: Byelorusskie (VNIISSOK, Moscow, Byelorussia), Velena, Russkie Chernye (VNIISSOK, Moscow), Alfred, Merkur (the Netherlands), Hangdown Grunkernig, Dreifach Weibe (Germany); four biopreparations: Novosil (natural regulator of plant growth and development), Rostok (natural humic preparation out of peat), Baikal (based on soil dwelling microorganisms) and Planriz (bacterial preparation based on live cells of Pseudomonas fluorescens culture, strain AP-33) The experimental pattern included treatment-free variants (control) and the variant with seeds pre-sowing treatment with biological preparations. All the preparations produced positive effects on seed productivity. Seed productivity increase per plant made up 4–17 % versus the control variant. The best indexes were obtained with the preparation Novosil, it was more effective when applied to the crop samples during the vegetation period. Over the years of the study the highest seed productivity of the crop was achieved in the variants with this preparation applied to cultivars Byelorusskie (390 g/m2), Alfred (330 g/m2). The data obtained allow to recommend bean cultivars mentioned and biological preparations to be used in practice under the conditions of West Siberia southern forest-steppe.
The paper considers the role of grain mass per spike as one of the main traits for which an individual selection is carried on for productivity in the hybrids of early spring wheat generations. Based on comparative examination of three generations in hybrid populations it is shown that the degree of phenotypic dominance can vary with crossing combinations and season conditions. The increased number of combinations with the trait super dominance was marked in the year unfavorable for moisture supply. The populations were identified which every spike exceeded the best parent for grain mass per spike. Individual selections were analyzed which were carried out in the second generation for the shift in offspring and heritability coefficient. It is shown that despite the high level of breeding differential, the selection efficiency for grain mass per spike is small, which is confirmed by minor correlation coefficient between generations F2–F3. The trait heritability realized in the selection groups varies from positive to negative values.
Based on the data of research conducted in the zone of risky seed production in Ukraine’s western forest-steppe that is characterized by poor natural soil fertility, higher acidity, heterogeneous soil cover and high hydrothermal coefficient (1.5–1.8) the paper provides economic estimation of winter wheat cultivars seed production, the wheat of ecological forest-steppe type of mid-early- and mid-ripening groups with different uncommon precursors and sowing dates. Profitability of the best seed stock production depended both upon precursors and cultivar characteristics and made up 54–73 for winter rape, 39–57 % for oat, the cost of 1 ton of seed output was 1.89–2.04 and 2.00–2.26 tsnd. grivnas, respectively. In acceptable sowing dates these indexes constituted 60–100 % and 1.57–1.97 tsnd. grn./t, but in late sowing dates they were 35–78 % and 1.76–2.32 tsnd. grn./t.
Silicon containing fertilizer “Siliplant” and mineral fertilizers are established to influence ornamental traits of lawn herbage. Increased projective covering of lawn herbage – meadow grass and red fes-cue – is marked with the preparation “Siliplant” and mineral fertilizers applied, particularly with their joint application. In the first and second years of research in dry and excessively humid vegetation periods the optimal results were obtained through the joint treatment with mineral fertilizers and the preparation “Siliplant”: the projective covering increased in the year of sowing by averaged 27.5 % in meadow grass and 25 % in red fescue versus the control, in the second year the covering went up by averaged 19.7 and 8.44 %, respectively. Mineral fertilizers applied increased the projective covering in the year of sowing on average by 22.5 % in meadow grass and by 20 % in red fescue, in the second year they did by 14.7 % and 6.25 %, respectively. The treatment with the silicon-containing preparation “Siliplant” increased the projective covering in the year of sowing by averaged 15 % in meadow grass and by 7.5 % in red fescue; in the second vegetation period the averaged effect of the treatment was by 6.58 and 1.51 % higher, respectively.
In West Siberia northern forest-steppe phytosanitary situation was examined in soya agrocoenosis in the years 1997–2013. The examination resulted in revealing a complex of the crop diseases. Among soil-borne infections the most common and deleterious are root rots caused mostly by fungi of Fusarium genus (F. oxysporum, F. oxysporum var. orthoceras, F. solani, F. solani var. coeruleumi, F. sambucinum var. minus, F. avenaceum, F. gibbosum, etc.). The disease progress exceeded the threshold of harmfulness through all the years of examination and reached over 70 %. Fusarium wilt was marked by considerable progress under dry conditions (over 70 %). Among leaf-stem infections, widely spread are fire blight (up to 35.8 %), peronosporose (37.0 %), and septoriose (32.5 %). Significant correlation of fire blight progress was revealed under warm humid conditions (r = 0.74 ± 0.21). The examinations carried out testify to the wide spread of the diseases in soya bean and the urgency to develop the complex of protective events to control them.
Colorado beetle was present on potato plantations through the entire vegetation colonizing and damaging them in a different way depending on the degree of tuber sets infestation with bare patch causative agent. The plants grown out of the tubers free from the infection onset show up a small amount of phytophage on the 33rd day after planting, but the plantations out of the tubers with bare patch sclerotia do 6 days later. The number of Colorado beetle imagoes was higher in the second case and made up 0.36 specimens per plant whereas in the first case it was by 1.3 less. Egg-laying began in the phase of complete shoots. The maximal number of egg-layings was observed in the plants grown out of healthy tubers, 0.29 egg-layings per plant, which is 1.2 times more than in the plantations out of the planting stock infected with bare patch sclerotia. The first larvae in the both cases emerged at the beginning of potato budding, but the number of the pests was close to and varied from 0.53 to o.57 pests per plant regardless the presence or absence of bare patch sclerotia on the planting stock. The peak number of larvae was observed in the phase of ripening: on the 50th day after planting and in the plants out of sclerotia-free tubers and on the 53rdday – in the plants out of sclerotized tubers. It was also established that in the first case potato was infested by the pest by 1.4 heavier than it was in the second case. The maximal number of summer generation imagoes was fixed in the phase of ripening. In this case the number of Colorado beetles on healthy plants was 1.2 times higher than that on the plantations out of bare patch sclerotized tubers. The crop attractiveness for imago in the field did not depend on bare patch presence on the planting stock. Larvae fed longer on the plants out of healthy tubers. The index was 1.8 times different. The intensity of leaf surface damage in potato bushes out of sclerotized tubers is by 11.2 % higher than in the plants out of healthy tubers. Tuber losses caused by Colorado beetle made up 29 %, by bare patch – 34 %, but the complex of harmful organisms concerned caused 46 % of the losses.
Herbicides and fertilizers applied increase chlorophyll accumulation, photosynthesis productivity and wheat yield. The total amount of chlorophyll during vegetation varied within 0.2–0.8 % of leaf dry matter. When exposed to ammonium nitrate and Dialen separately and jointly, chlorophyll content increases during vegetation, with joint application the increase being much higher. Chlorophyll content maximal value was in the phase of tillering, minimal one was in the phase of shooting. Photosynthesis maximal value was observed in all ontogenesis phases in the variant with ammonium nitrate and Dialen joint treatment. Under separate expositions to ammonium nitrate and Dialen wheat yielding goes up, but their strongest effect can be seen in the variant of joint application. The wheat yielding increase is due to the higher number of productive stalks, grains number per plant and 1 000 grains weight.
Fungicide treatment of seedlings is the method of prompt response to negative changes in the phytosanitary conditions of agrocoenosis. Their treatments are to be economically and ecologically justified. The aim of the research was to study technological techniques of applying plant growth regulators, biopreparations and fungicides to spring wheat in order to choose the most effective of them, improving phytosanitary conditions of seedlings and increasing crop productivity under Kurgan region conditions. Field experiments were conducted in Central test-field of Kurgan Research Institute of Agriculture in 2004–2013. The objects of research were soft spring wheat cultivars: Omskaya 18 (in 2004–2006), Omskaya 36 (in 2007– 2013). Bare fallow was the precursor. The field experiments conducted showed different efficiency of the preparations of fungicide effect on spring wheat under altering intensity of agrocoenosis damage. Yielding went up by 3.0–18.7 % versus the control. Biological efficiency of the preparations was determined by reacting substance and degree of wheat leaf damage from 46.2 to 97.7 % in the years of epiphytoties and from 10 to 83.5 % under the conditions of moderate progress of leaf-stem infections. The treatment with fungicide preparations is considered unreasonable in the years of depressive manifestation of air-borne and above ground diseases.
Diagnostic signs of the disease examined in 18 onion and rhizome salad onion species include the symptoms such as leaf chlorosis, leaf blade tips dying off, growth inhibition, withering, above-ground mass drying, rot and flower heads mummification, pedicle breaks, root and bulb rots. Light coats or sporiferous balls of pale gray, pinkish and orange color appear at the base of pseudostalk, leaves or their axils, pedicles and flower heads, which is characteristic of sick plants. For the first time, the symptom of “white spires” was revealed in salad onions attacked by fusariosis, which can be used as a marker in phytosanitary monitoring. It is marked that fusariosis infection can mask on plants looking as dark coats of hyphomycetes of Stemphylium, Alternaria and Heterosporium genera. Bright reddish spore carriers of disease causative agents are similar to small well-opened pustules of rust causative agents, Puccinia allii. It is established that phenophases of budding and flowering are the main dates to diagnose the disease visually on salad onions under the conditions of West Siberia forest-steppe.
The paper studies comparative productivity of sown unispecies grasses and grass mixtures under different cover crops. The grasses under barley cover developed more intensively. Haulm stand density and perennial grasses height under barley cover are higher than those under grass mixture cover, which determined richer yielding capacity of perennial grasses. However, the effect of cover crop on productivity of sown perennial grasses is minor and this was concluded, on average, from two experiments laid for 5 years. A considerable influence of cover crop was marked in the first years of grasses life. Under the cover of barley the productivity of perennial grasses was much higher than it was under cereal-legume grass mixture. After the third year of life the difference in yielding capacity of sown perennial grasses under different cover crops gradually smoothes over and becomes minor. Consequently, cover crop does not influence productive longevity of grasses. The grass mixture of lucern and rump is distinguished by the highest productivity and that of cereal grasses and Galega orientalis is by the lowest.
The data on the study of new cultivars and hybrids of vegetable marrow squash are given. It is revealed that all the experimental plants grew and developed similarly. Their undergoing the phases of development differed in 1–4 days. It is revealed that the experimental plants refer to the early-ripening group. Maximal yielding was marked in the third decade of July: from 38.26 to 57.3% of the total yields. In all the rest of the decades yields were distributed roughly equally. The following cultivars and hybrids were the first to stop producing yields: WS 750 F1 , Yantai F1 № 5, Yantai F1 № 4, Yanhu F1 10–4, Yakor (anchor). Maximal productivity in the second and third decades was marked in the cultivars Aral and Sukha and in the hybrids Bianca F1 , Verdi F1 , Yanhu F1 10–5. Mathematical processing of the total productivity showed that differences in the experiment are essential. Difference between the variants makes up from minimal values (LMD) up to almost 5-fold differences. The highest total productivity is marked in the cultivars: Aral (28.23 kg/m2 ) and Sukha (22.33 kg/m2 ), the least one – in the hybrids WS 750 F1 , Yantai F1 № 4, Yantai F1 № 5 (2.95, 3.12 and 6.93 kg/m2 , respectively). Standard output in most variants of the experiment made up 100%, except for the hybrids Bianca F1 , Yantai F1 № 4, Yantai F1 № 5. Standard output in all the variants is 11. 18 and 14% accordingly lower than the control. Degustation evaluation of all the experimental cultivars and hybrids was high: from 4.34 points in the hybrid Yantai F1 № 4 to 4.95 points in the hybrid Bianca F1 . All the experimental cultivars and hybrids are resistant to the complex of diseases, except for the hybrids Yantai F1 № 4 and № 5.
ЖИВОТНОВОДСТВО
VETERINARY SCIENCES
The search for new preparations effective against viral causative agents in cattle infections is the most important objective of the modern veterinary pharmachology. The virus of infectious bovine rhinotracheitis is widespread on the farms of Siberian region and plays an important part in animal pathology causing big economic losses in the modern livestock-breeding. Antiviral activity of the preparations referred to the group of probiotics is little studied. The antiviral activity of vetom 1.23 against BHV-1 is determined in vitro. The antiviral activity was assessed by reduction of the virus; the assessment was conducted after the virus’s having interacted with vetom 1.23. Graft cell culture of MDBK calf’s kidney was used in the experiment. Avirulent strain ТК-А was used as the virus-test. vetom 1.23 was introduced in the maximal tolerant concentration (62.5 mm3 / cm3 ). The experimental outcomes showed that vetom 1.23 at the dose recommended for the examination and introduced (maximal tolerant concentration) appeared to be little effective against BHV-1, it was poor inhibition in virus multiplication that was observed; the virus titer went down by 0.13–0.5 log10. However, the preparation manifested expressed antivirus effect in higher concentrations.
Supernatants of monocultures of lactic acid feed addivitve (LFD) based on the microorganisms of the probiotics Lactobacillus acidophilus L-41, Streptococcus termophilus В-41, Bifidobacter longum Б-41, Propionobacterium freudenreichii shermanii 76 enhance antibiotic sensitivity in E. coli ATCC 25222 and S. enteritidis 182 to different extents from the 1st to the 30th day since the start of cultivation when contacting for 18–20 hours. The highest growth of sensitivity in E. coli ATCC 25222 to antibacterial preparations (from 41.7 to 54.2%) is marked after the cultivation with probiotic cultures aged 20 and 30 days. The contact of the supernatants of all the LFD monocultures concerned and obtained on the 10th and 30th day of their cultivation with S. enteritidis 182 followed by antibiotic sensitivity determined causes the diameter of microorganisms growth delay to increase more than it does in the 20-day cultures. The examinations conducted offer the challenges to study the influence of metabolites of probiotic microorganisms in LFD composition for antibiotic sensitivity of opportunistic pathogenic microflora, which assists in solving the problem of antibiotic resistance and consequently, enhancing the efficiency of treatment-and-prophylactic veterinary events in livestock-breeding.