In recent years, interest in non-traditional methods of farming and plant growing has increased, which include the widespread use of biological methods of plant protection and nutrition, allowing significantly reduce the use of pesticides and reduce fertilizer rates. Therefore, there is a constant search and selection of highly effective and competitive formulations that, in growing technologies, ensured an increase in yield and the quality of the grown products. It has been established that the reasons for obtaining seeds of low yielding and sowing qualities of winter wheat in the zone of excessive moisture in the Western Forest-Steppe of Ukraine are lodging of crops and the development of harmful diseases. These negative phenomena can be prevented through the use of the Vympel-2 growth regulator and Oracle microfertilizer colofermin copper, both when separately and simultaneously introduced into the ejection phase of the tube. Under the influence of these formulations, resistance to lodging of plants increases, the spread of diseases decreases: powdery mildew, leaf septoria, and dark brown rust. The increased spike growth and the best filling of winter wheat grains occur due to the shortening of the vegetative part of the shoot, which contributes to a significant increase in productivity - 0.45–0.55 t / ha. Due to optimal growth and development, seeds of high sowing qualities are formed on maternal plants. The most effective is the tank mixture of the Vympel-2 growth regulator (0.5 l / ha) with microfertilizer Oracle colofermin copper (1.0 l / ha) against the background of N30P90K90 with the phased introduction of nitrogen according to N30P90K90 at the IV and VII stages of organogenesis, which reduced development diseases on plants compared with the control (without treatment of sowing) by 15.4% (powdery mildew), 10.8 (leaf Septoria) and 10.4% (dark brown spotting).

To improve the genetic-breeding characteristics of maternal forms, to increase their adaptive potential in breeding hybrids of agricultural crops (sorghum, African millet, corn, rice, sunflower, winter rye, etc.), various types of CMS are used. The article presents the results of studying the collection of CMS sorghum lines obtained on the basis of new sources of sterility (A1, A2, A3, A4, A5, A6, 9E and M-35-1A) by agronomic characteristics. On the experimental field of the Institute in 2014-2018. 21 lines were sown in a wide-row way with row spacing of 70 cm and a density of standing of 100 thousand plants per 1 ha. The allocation of plots of 7.7 m2 is randomized, in triplicate. The use of cluster analysis for the minimum of Euclidean distances allowed us to group CMS lines according to similar studied features: the 1st group includes 9; 2nd - 4; 3rd and 4th - 3 each; 5th and 6th - 1 line each. One-way analysis of variance confirms the validity of differences between groups by 18 selection criteria, with the exception of the extension of the panicle leg. A significant variation in vegetative (V = 11.0-21.6%) and generative traits (V = 13.2-28.4%) was found. The smallest coefficient of variation was revealed by the duration of the seedling-flowering interphase period — 4.8%, which indicates the maturity of most lines (in A2 KVV 181, A2 Suzern light, A2 AGS - 46.6 days). A five-year test of the source material made it possible to identify productive lines of the 5th and 6th groups (grain productivity 5.07-5.36, biomass - 18.30-20.70 t / ha); weakly growing lines of the 3rd group (total and productive bushiness 1.12-1.14), low-growing lines of the 1st group (plant height 81.0 cm). A1 Efremovskoye 2 is distinguished by tallness (143.3 cm), large sizes of flag and largest leaves (areas of 150.0 and 254.2 cm2, respectively). The selection of sterile lines with predetermined traits helps to optimize the selection process and their targeted use in crossbreeding programs.

The results of field studies for 2017 - 2019 are presented. yields of perennial grasses sown at different times of the growing season. Spring, summer, and winter sowing periods were compared. Alfalfa, clover, rump, and also their mixtures were sown in 2017 under the cover of barley. The value of the cover crop yield of spring and summer sowing periods did not differ significantly and amounted to 4-5 t / ha of absolutely dry matter. Winter barley crops have not formed. On average, over 2 years of use, the highest yields were observed in alfalfa-crust grass mixtures - 3.4 t / ha of absolutely dry matter. The lowest yield was obtained in the single-species seeding of the rump. Correspondingly, in the spring, summer and winter periods of sowing, the yield of rump was 1.6; 1.1 and 1.3 t / ha. With a late sowing period, the yield of perennial grasses is significantly lower compared to spring and summer. With winter sowing periods, the yield was the highest for grass stands of alfalfa and alfalfacrust grass mixture - 2.3 and 2.4 t / ha. It should be noted that in the second year of use, the yield by the sowing dates in single-species crops and grass mixtures is leveled. The winter crops of perennial grasses in the first year of use formed a low yield. Only in the second year (third year of life) the productivity of perennial grasses of winter sowing began to increase. Consequently, in the area under perennial grasses of the winter sowing period, during one growing season (the next year after sowing), the crop was not actually formed. Based on the data obtained, production can be recommended for spring and summer planting of perennial grasses under the cover of barley. The winter sowing period provides economically valuable crop yields only by the third year of life.

The purpose of the study is to identify the dependence of spring wheat productivity on agrometeorological indicators of the vegetation period at various levels of nitrogen nutrition in the central forest-steppe of the Ob Region. Wheat of medium early varieties was grown in a wheat-barley crop rotation at doses N0, N30, N60 and N90 against the background of a full range of plant protection products. The correlation between wheat productivity and agrometeorological indicators was evaluated based on the results of 13 years of research. To characterize the hydrothermal conditions, we used the reserve of productive moisture in the soil, the amount of precipitation and air temperature, the Selyaninov hydrothermal coefficient , and the Ponko wetting coefficient. Over the years, grain yield varied from 7.2 to 43.6 kg / ha, Selyaninov coefficient - from 0.18 to 1.67. The highest correlation coefficients (0.7–0.8) were obtained between wheat productivity and indicators of hydrothermal conditions in June - July. Moreover, with the amount of precipitation of this period, the dependence was positive, with air temperature - negative. No reliable relationship was found between grain yield and spring stock of productive moisture in the soil. The tightness of the relationship between wheat productivity and the Selyaninov hydrothermal coefficient and Ponko hydration coefficient was the same, with the highest correlation coefficients also obtained for June – July. The increase in wheat grain from nitrogen fertilizer increased significantly as the hydrothermal conditions of the growing season improved. Based on the obtained values   of the fertilizer nitrogen payback by increasing the crop, it was concluded that the current price ratio - the sale of grain by the farms and their purchase of nitrogen fertilizers (approximately 1:7) is unfavorable for the development of intensive crop cultivation technologies.

A description of a promising new variety of vegetable beans suitable for cultivation in the conditions of the Siberian region is presented. Work on the evaluation of promising samples has been carried out at the Novosibirsk State Agrarian University since 2000; annually, samples are compared with the best varieties of Siberian breeding. A new promising variety F135 (Nurse) was selected, which was transferred to the state variety test. A promising variety of Nursing was bred at the Department of Selection, Genetics and Forestry of Novosibirsk State Agrarian University by individual selection from a hybrid population by crossing highly productive, adaptive, early ripening varieties of German vegetable beans Maxi and Rocquentcant. The data on the evaluation of a promising sample of vegetable beans by qualitative and quantitative parameters are presented. The assessment was carried out in accordance with the recommended methods. The advantages of the new variety Nurse by the main economically valuable characteristics are established in comparison with the standard variety. The sample belongs to the early ripeness group. The period from full germination to technical ripeness is 40 days. A promising sample of the Nurse has high indicators of manufacturability and productivity when grown in Siberian conditions. The variety is characterized by the ease of separation of the green shoulder blade during the period of technical ripeness, the friendly formation of high-quality green beans. The height of the plant reaches 45 cm, and the height of attachment of the lower bean is more than 12 cm. The green shoulder blade is characterized by good taste, parchment layer and fiber in the seam are not formed. Biochemical analysis of the green scapula showed that the promising Nurse grade exceeds the standard for the content of protein, dry matter, ascorbic acid and sugar.

The antagonistic activity of lactobacilli in the intestine in relation to various enteropathogenic microorganisms can vary within wide limits, including depending on the species composition of the lactobiota of the intestine. The purpose of this work was to determine the antagonistic activity of representatives of the order Lactobacillales isolated from chickens in poultry farms with different levels of Salmonella infection. The test object was the chickens of the parent herd and broiler chickens of crosses Ross 308 and Hubbard F - 15 from five poultry farms. Three poultry farms were characterized by a low level of salmonella infection in birds (less than 5% for cloacal swabs in PCR and the absence of salmonella isolation from food products). Two poultry farms were characterized by a high level of Salmonella infection (poultry infection by cloacal swabs of more than 10% and official salmonellosis disadvantage due to isolation of Salmonella cultures in food products). The level of infection was evaluated by real-time PCR after preliminary subculture of cloacal swabs on Shadler’s broth. The antagonistic activity of lactobacilli and related bacterial species isolated from the same chickens was carried out in co-cultivation tests on the Shadler broth with subsequent identification of salmonella on the RVS broth. Poultry farms with low Salmonella infection were characterized by the presence of L. reuteri as a major component of intestinal lactobiota and had a higher antagonistic activity against more Salmonella cultures (odds ratio (OR) 17.33 (CI 95 = 5.99-50.07776))

One of the main links in the system of measures to eliminate brucellosis is the timely and reliable identification of infected animals. In the serodiagnosis of this disease, reactions such as RBPT, CFT (RCFT) and AT are widely used. Recently, various variants of ELISA tests find their application. Both in traditional reactions and in ELISA, lipopolysaccharides of smooth strains of Brucella spp. act as the main antigen, which complicates the differentiating infected from vaccinated animals. In addition, these tests do not always give objective results due to the cross-reactions of Brucella with other gram-negative bacteria. In this regard, the results of studies devoted to the determination of the diagnostic value of the protein components of the pathogen deserve close attention. The diagnostic potential of Brucella recombinant outer membrane proteins (OMP19, OMP25, OMP31) and the periplasmic protein - superoxide dismutase (SOD) in indirect ELISA was studied. The research results showed that cows 10 months after revaccination with  B. abortus 19 in 60% of cases gave positive reactions by RBPT and indirect ELISA based on Brucella OMPs, while antibodies in indirect ELISA/SOD were detected only in 4% of the population. About one third of the suckling calves kept on with their mothers revaccinated against brucellosis had specific antibodies to Brucella OMPs by 6 months of postnatal ontogenesis. The use of individual recombinant proteins in indirect ELISA reduced the sensitivity of the test in serological studies of mother cows and their suckling calves. In serum of seropositive cows from epizootic foci of brucellosis, antibodies to Brucella OMPs as well as SOD were detected in 96.7-100% of cases. Thus, the obtained results provide the basis for further research to determine the serological potential of SOD in the differentiation of Brucella-infected from vaccinated animals.

The data on the methods of modeling polyetiological stress in laboratory mice and its effect on the development of the inflammatory response and the nature of pathological and anatomical changes are presented. At the first stage, two groups of animals simulated polyetiological stress. For this, the animals were infected with micromycete intraperitoneally and left stationary for several hours a day. After that, infection was assessed by detecting micromycete in the peripheral blood of animals. At the second stage, the degree of occurrence and the nature of pathological and anatomical changes were studied with different methods of micromycete administration. It was noted that as a result of the simultaneous exposure to two stress factors, micromycetes in the peripheral blood were detected on the 8th day of the study. At the same time, the influence of one stress factor led to the generalization of the process only on the 17th day. With different methods of infection in laboratory mice, characteristic changes were revealed in the internal organs, which in all groups of animals were represented mainly as single and multiple abscesses of different localization, as well as fistulas in the left caudal part of the peritoneum. Abscesses in the region of the left forearm were filled with characteristic purulent contents: a curdled mass of light gray with an unpleasant odor or odorless. These pathological and anatomical changes were noted in 90% of the animals of their total number in the experiment. The introduction of R. syclopium followed by the exposure of white mice to IMO stress leads to 100% death of animals on days 1-2 from the onset of stress. Animals that were only injected with P. cyclopium remained alive until the end of the study.

The results of studies of indicators of nitrogen metabolism in the blood serum of the descendants of four bulls at the age of 12-13 months are presented. The studies were conducted in the South of Western Siberia at Vaganovo OJSC on the livestock population of Holstein breed. The creatinine concentration in the blood serum samples of the sons was determined using a set of reagents from Vector-Best (Russia). In the regions of animal breeding, constant monitoring of water, soil, feed, animal organs and tissues is carried out. It was established that in the areas in which the studied breeds were bred, the content of macro- and microelements did not exceed the MPC. The level of serum creatinine in the examined animals on average for all groups of sons was 308.2 ± 24.1 μmol / L, which exceeds the generally accepted values   of this indicator. However, taking into account age and sex and breed characteristics, taking into account data on the overall health of the animals examined, information on the ecological well-being of the breeding zone, the results can be considered as reference values   for healthy Holstein bulls in Western Siberia. It was shown that in the blood serum of the sons of some fathers, the creatinine content was 1.7 times higher (364.2 ± 52.7 μmol / L) than in the descendants of other manufacturers (p> 0.95). A group of half-siblings was found, which were characterized by a low hereditary predisposition to creatinine content. Discovered differences in the creatinine content in the blood serum of sons reflect the differentiation of bulls and indicate genetically determined features of the functioning of the homeostasis system. In this case, most likely, there is a different ability of descendants to adapt to environmental and climatic conditions.

The effect of the probiotic preparation Vetom 1.2 on the basis of Bacillus subtilis VKPM B-10641, Bacillus amyloliquefaciens VKPM B-10642 and Bacillus amyloliquefaciens VKPM B-10643 in different doses on the correlation interactions between the parameters of turkey hemoglobin exchange, blood erythrocytes and blood red blood cells direct and total bilirubin, total protein and serum albumin was studies. Correlations were calculated using Spearman formula, the reliability of correlations was checked by Student. The formulation was used for turkeys once a day for 30 days in doses of 12.5; 25; fifty; 75 and 100 mg / kg body weight. It was found that Vetom 1.2 in minimal doses leads to an inverse correlation between iron and total bilirubin, direct bilirubin, total protein and albumin, an increase in the concentration of the formulation leads to the disappearance of this effect with a gradual subsequent decrease in this indicator. Vetom 1.2 in doses up to 50 mg / kg leads to an inverse correlation between the concentrations of hemoglobin and direct bilirubin, total protein and serum albumin, at a dose of 75 mg / kg there is a direct correlation between these indicators, and at a dose of 100 mg / kg returns a negative correlation. The probiotic preparation Vetom 1.2 leads to a direct dependence of the concentration of bilirubins (total and direct) in serum on the concentrations of hemoglobin and red blood cells when used in most doses, except 75 mg / kg, which reduces the correlation.