AGRONOMY
Due to the growing variety of vegetables, the impact of genotypic parameters of plants on pest resistance and efficiency of biological specimens is of high importance when selecting and creating new varieties and hybrids. The researchers found out the significant differences in stability of parental forms, hybrids and cucumber varieties of the Siberian selection in relation to twospotted spider mite. The paper finds out relatively resistant parental forms - ZhL-4 and GP-61/b, and also samples of the paternal form - GF-7 and a maternal line - ZhL-9 damaged by a phytophage in a strong degree have been defined rather steady against a phytophage. High acaricidal effect of Phytoverm was found on all studied varietal samples, however the most effective biospecimen restrained development of the pest on plants of relatively resistant to phytophage of female line ZL-4. Reproduction of the twospotted spider phytoseiulus did not depend on genotypic features of parental forms, varieties and hybrids of cucumbers. The number of acarifages increased by 7.8-8.2 times in two weeks after appearance on the plants inhabited by spider mite in comparison with the initial number, as a result of which the damage rate of plants by the pest of most cultivars decreased, and in male forms practically did not change (GF-19) or slightly increased (GF-7). According to biochemical parameters, cucumber varieties with different degree of resistance to phytophage differed in dry matter content. Non-resistant to twospotted spider mite female forms LL-9, LL-12/1 and Nadezhda variety contained the lowest concentration of dry matter (5.4%), the highest amount of dry matter was observed in female line LL-4, which has the highest pest resistance (6.0%). Since the inherited traits are predominantly transmitted by the maternal component and the paternal form enhances these properties, it is important to assess not only the yield and flavour qualities but also the degree of resistance of parental forms to pests when selecting cucumber varieties and hybrids.
Field research was carried out in 2018-2019 on the premises of Omsk Agrarian Research Centre, exactly the breeding crop rotation of the laboratory of durum wheat breeding as well as at the reference point of seed production in the steppe zone in the village of Novouralsky Tavrichesky district of Omsk region. The experiments were conducted in a complete fallow. The area of the plots was 10 m², repeated four times, the location was random. Weather conditions during the vegetation period of 2018 were contrasting both in terms of precipitation and temperature. Grain formation took place in late August - early September, which had a significant impact on grain quality. In 2019, atypical meteorological conditions were observed: favorable in the first vegetation period and arid - in the second (Volga type of drought). The productivity of durum wheat lines was estimated in terms of adaptive capacity and stability. Scientific data on the parameters of grain quality and disease resistance are highlighted. Yields of durum wheat lines varied considerably depending on agro-climatic conditions of the year and cultivation background. The best conditions for the growth and development of genotypes were set up in the southern forest-steppe zone of Omsk, the maximum crop yield was observed in the Gord. line 08-67-1, Gord. 09-68-1, Gord. 05-12-7(v), Gord. 08-107-5. The general adaptive ability is close to zero, that shows stability of genotypes in external environment. Specific adaptive ability revealed in the lines: Gord. 10-33-3, Gord. 10-8-4, Gord. 11- 70-7, Gord. 11-77-8, Gord. 11-99-1. The authors highlight the following promising lines according to the experimental results: Gord. 05-12-7(v), Gord. 08-107-5, Gord. 09-68-1, Gord. 08-67-1. Selection assessment of promising lines was carried out on the indicators of grain quality and disease resistance. The following parameters are seen as good quality indicators at the Gord line 11-70-7: weight of 1000 grains is 49.75 g and high resistance to stem rust (6%). According to the quality of grain and weight of 1000 grains, Gord. line 09-68-1 is better than Zhemchuzhina Sibiri, affection of brown and stem rust is minimal that is interesting for selection procedures. The following varieties are valuable from the point of view of breeding, stability of genotype, high productivity, quality of grain and pasta and resistance to disease: Gord. 05-12-7(v), Gord. 08-107-5, Gord. 09-68-1, Gord. 08-67-1, Gord. 11-70-7, Gord. 11-99-1.
The paper shows the results of field experiments conducted in 2017-2019. The experiments were devoted to photosynthetic indices of alfalfa in relation to the type of nitrogen nutrition and symbiotic activity of crops. The experiments with alfalfa blue-hybrid were carried out in environmental conditions of the piedmont zone of RNO-Alania on chernozem leached soil with close occurrence of gravel. The researchers compared mineral and symbiotrophic types of nitrogen nutrition of alfalfa plants; they applied mineral nitrogen fertilizers and biospecimens on the basis of rizobium. In natural conditions (control group) the crops formed photosynthetic potential from 1701.3 thousand m²-days/ha per year of sowing to 3624.3 thousand m²-days/ha in the second year of sowing. Crop rates in the third year had intermediate values between the first and second years of crop use. The effect of starting doses of nitrogen fertilizers was observed during the sowing year in its early stages, when the symbiotic system is still underdeveloped and inactive. When estimating the annual average photosynthetic potential, starting nitrogen doses gave an advantage of only 1.6%. Presowing inoculation of seeds by active strains of strawberry bacteria provided the maximum positive effect, while high mountain strains of rizobium were more effective. Average annual parameter of photosynthetic potential varied from 2699.1to3189.4 thousand m² days/ha, increasing with the improvement of nitrogen nutrition. Inoculation of seeds by high-altitude strains of tuber bacteria increased photosynthetic potential to its maximum values or by 18.2%. In the same variant, as well as in the variant with the starting dose of nitrogen, the maximum values of net productivity of photosynthesis were achieved, which exceeded the values of the control variant by 0.09-0.11 g/m²- day.
Natural hayfields in floodplain meadows are seen as a source of high quality forage for animal husbandry. Due to annual silt and plant seed load, these agrocenoses are able to support themselves and self-repair. However, intensive use depletes them. Thus, the average hay yield decreses from 3.5-4.0 to 1.2-1.5 t/ha. The return of some elements of mineral nutrition, as well as the use of various microfertilizers and growth stimulators should bring the yield of floodplain meadows back to the level of natural biological situation. Sysola and Vycheda vareties with application of mineral fertilizers (R45К45+N30 and R45К45), two types of microfertilizers (boron and molybdenum), three growth stimulators (Verva, Epin-extra, Gumat) and two specimens on the basis of pectin polysaccharides with stimulating effect (PAo and HSw) were developed at the Institute of Biotechnologies in Agriculture of Komi FIC NTs UD RAS (Komi Republic, Syktyvkar) and tested in floodplain meadows of the river. The authors explored various combinations of these options. The experiment was conducted at three pilot sites in 2008-2010; 2013-2015 and 2016-2018. As a result of the research, it was found out that floodplain meadows intensively used as hayfields are most in need of replenishment with phosphorus and potassium. Application of various microelements and growth stimulators in the pure form increased yields by 30-40 % with a comparable energy coefficient and lower energy intensity of feed. The most effective method was the variant with application of Verva specimen together with the full dose of mineral fertilizers. This method increased the yield by 2 times, provided the maximum yield of forage units - 1.5-2.3 thousand / ha. At the same time, the energy intensity of forage was 20% lower than in the control variant.
The researchers conduct the field stationary experiment with the use of mineral fertilizers and straw in the grain and steam crop rotation with the withdrawal field of alfalfa and study the number of microflora in the rhizosphere of winter wheat of the new variety Priirtyshskaya after treatment of seeds with biospecimen of complex effect - risoagrine. The highest number of useful crop groups of microorganisms was observed in the variant with inoculation of crop seeds by risoagrine on the basis of applying mineral fertilizers, as well as in combination of inoculation techniques, application of mineral fertilizers and straw (N15P23 + straw + inoculation), respectively, 444 and 355 million UU/yr with 217 million UU/yr in the control group. In the variant with inoculation of winter wheat seeds by mineral fertilizers (N15P23 + inoculation), the number ofoligonitrophils and bacteria, mineralizing mineral phosphates, increased by 2.2 times, nitrifiers - by 60%, microorganisms that utilize organic nitrogen compounds on MPA - by 39, consuming mineral nitrogen on CAA - by 73% compared to the control group. The celluloseolytic soil activity under winter wheat sowing in variants N15P23 + inoculation and N15P23 + straw + inoculation increased to 66.5-67.0%, exceeded the control group by 1.7 times. The highest increase in the crop’s grains was observed in the combination of mineral, organic (straw) and bacterial (rizoagrine) fertilizers - 40.3% in comparison with the control group. Additional nitrogen removal by winter wheat crop due to the activity of associative diazotrophs varied from 6 to 16.5 kg/ha. Correlative relations of high (r=0.84-0.91) and average (r=0.62-0.72) degree of microorganisms in the rhizosphere were observed among the indicators of crop yield and number of microorganisms. The closest correlation took place between the value of winter wheat grain yield and the number of bacteria growing on MPA, including ammonifiers, and the yield and number of nitrifying bacteria.
The UN Food Agency announced 2020 as International Year for Plant Protection. About 40% of crop yields losses are caused by pests and diseases. Due to this, millions of people suffer from hunger. Therefore, there is a need to seek for and select environmentally friendly and highly effective products that will increase yields and the production quality. In 2017-2018, in the Novosibirsk Region, it was shown in field trials and production experiments that the biopreparation Phytop 8.67 based on the bacteria of the genus Bacillus has antagonistic activity in relation to carrot alternativeariosis (the causative agent is Alternaria dauci carrots, reduces the plants prevalence during the vegetation period, and also has a growth stimulating effect. The prevalence of brown leaf spotting decreased by 4.8 times, and the biological effect of Phytop 8.67 in two years with respect to the disease progress was 63.2 (after 10 weeks) and 78.4% (after 12 weeks). In the experimental variants, the plants were statistically more reliable than the control ones and were more foliated on all the dates of accounting. Application of biospecimen increased 1 root crop weight by 1,7 times in the control variant, in finedelivery experiment and by 1,4 times - in industrial one. Due to this, the authors recommend applying Phytop 8.67 specimen as a fungicide, a growth stimulator in order to increase carrot productivity.
VETERINARY MEDICINE AND ANIMAL SCIENCE
Blood inflow of Holstein breed in Mari El cattle breeding, which began in 80s of the last century, had a positive impact on the dairy productivity of Black-and-White cattle. The research explores the impact of Holstein breed bloodline and lineage on the main indicators of cows’ dairy productivity in OAO Ovoshchevod. The productive age increasing, the live weight of cows in the main herd increased as well while the animals were relatively small. The cows of the eighth and ninth calves had the maximum live weight. On average, the cows with the third complete lactation (6791.5 kg) had the highest milk yield. The mass fraction of fat was on average higher than the basic fraction and was equal to 3.71%. The increase of milk fat concentration was observed in cows of the fifth and sixth lactations, with the offsprings of Black-and-White bull lines being the most fatty. There is a direct correlation between milk yield and the degree of blood flow in the Holstein breed. The fattiest milk cows were those with a blood content of 19%, while the cattle with a blood content of 75% had the lowest fat concentration in milk. According to the hospitalization data, most of the cows in the herd (including the first heifer cows) belong to the R. Sawring line. The female offsprings of Adam, Rikus and M. Chiftein bulls differed in the most productive longevity. The most abundant were cows belonging to M. Chiftein line. Female offsprings of Black-and-White bulls were inferior to the Holstein ones in average by 324 kg of milk. This feature was traced from first to fifth lactation. In the lactation section, the most fatty-milk cows of the Rikus and M. Chiftein lines were the fattest. As the share of blood in the Holstein breed increased, the milk yield per cow in the main herd increased as well. Thus, for cows with blood content of 18.75%, the average milk yield for all lactations was 6313 kg of milk. Further blood inflow of Holstein bulls led to this parameter increasing by 4% on average for cows with blood content of 25 and 37.5% for Holstein breed.
The process of improving the breeding and productive qualities of dairy cattle is impossible without the selection of servicing bulls with high genetic potential and breeding value. The paper investigates the breeding value and genetic potential of servicing bulls whose bio-products are used to inseminate dairy cattle in the Republic of Mari El. The author finds out that the population of bulls is of high quality and belongs to five genealogical lines: Vis Back Aydial 1013415, Montwick Chiftein 95679, Sawring Reflex 198998, Seling Trijun Rokita 252803, Pabst Governer 882933. Bulls, obtained from highly productive ancestors and have a high genetic potential in terms of milk yield (from 7513 to 13361 kg) and mass fraction of fat in milk (from 3.87 to 4.43%). The female offsprings of the investigated servicing bulls have rather high level of milk productivity (milk yield is 7017 kg on average, mass fraction of fat is 3,94, protein - 3,08%). The paper highlights that the highest level of dairy productivity was achieved by the descendants of the bull Yasa-M 462771. Their milk yield for the first lactation was 8185 kg, mass fraction of fat was 3.93%, protein concentration was 3.11%. The most part of investigated bulls have high breeding value according to all studied milk productivity parameters. According to the milk yield, it varied from 96.1 to145.5%, the mass fraction of fat – from 100 to 101.8, protein -from 98 to 101.6%. 10 servicing bulls as Artist, Butembo-M, Koldun, Leksaid, Morzhik, Norman-M, Auckland-M, Sayan, Spartak and Yasa-M have relative breeding value on all studied indicators which was above 100%. The most valuable servicing bulls were observed as Yas-M 462771 and Koldun 103.