MORPHOLOGICAL AND IMMUNE HYSTOCHEMICAL RESEARCH ON PIGS’ ADENOID TISSUE associated with conjunctival TISSUE OF PIGS
Abstract
Pig’s conjunctiva has local immune system, which is conjunctiva associated lymphoid tissue. It is represented by lymphoid cells and lymphoid tubercules as well as high endothelium venules in subepithelial connective tissue of conjunctiva connected with conjunctiva epithelium. The research shows that conjunctiva associated lymphoid tissue is a part of mucosal associated lymphoid tissue and morphologically similar to gut-associated lymphoid tissue and bronchoassociated lymphoid tissue. Immunohistochemical research methods are essential part of morphological research on lymphoid tissue of different animals. There are no data in literature about immunohistochemical research of pigs conjunctiva associated lymphoid tissue that are aimed at revealing main immune cells and their distribution. Immunohistochemical staining of paraffin section of pigs’ lids fixed in formaline by means of standard immunohistochemical methods (ABC and PAP) with application of primary antibodies to markers CD3, CD79а и PCNA showed positive reactions with Т-lymphocytes, В-lymphocytes and blast cells in conjunctiva associated lymphoid tissue. The paper describes location of Т-lymphocytes, В-lymphocytes and blast cells in conjunctiva associated lymphoid tissue. The authors found out morphofunctional changes in pigs’ conjunctiva associated lymphoid tissue in dependence of their age.
About the Authors
З. Каландарова
Кыргызский национальный аграрный университет им. К. И. Скрябина
Russian Federation
Russian Federation
М. Райнахер
Институт ветеринарной патологии
Russian Federation
Russian Federation
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, MORPHOLOGICAL AND IMMUNE HYSTOCHEMICAL RESEARCH ON PIGS’ ADENOID TISSUE associated with conjunctival TISSUE OF PIGS. Bulletin of NSAU (Novosibirsk State Agrarian University). 2017;(2):86-93. (In Russ.)
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