HISTOLOGICAL RESEARCH AND IMMUNE HISTOCHEMICAL TEST OF THE CATTLE LYMPH NODES
Abstract
The article reveals the results on the cattle lymph nodes’ histological structure and cell composition by means of carrying out histological methods, immune histochemical tests and applying polyclonal antibodies and mononclonal antibodies. The author tests mediastinal lymph node, hepatoportal lymph nodes and mesenteric lymph nodes belonging to 12 animals of the cattle aged from 1.5 to 7. The researcher applies Polyclonal Rabbit Anti-Human T cell, CD3, (PAP), Monoclonal Mouse Anti-Human B-Lymphocyte Antigen (BLA. 36) (ABC), Monoclonal Mouse Anti-Human Myeloid/Histocyte Antigen, Clone MAC 387 (PAP), Monoclonal Mouse Anti-Proliferating Cell Nuclear Antigen, Clone PC10 (PAP) sera and makes conclusion they can be applied in immune histochemical test when dividing T-lymphocytes, B- lymphocytes, macrophages and cell growth in the cattle paraffin sections. The article describes the lymphocytes structure and defines location, intensity and spreading of T-lymphocytes, B-lymphocytes, macrophages and cell growth in T-zone and B-zone of lymph nodes’ cortex and medullary substance. The publication declares the data received can be applied as fundamental data when carrying out morphological research on the cattle lymph node pathology.
About the Author
G. K. Kasieva
Кыргызский национальный аграрный университет им. К. И. Скрябина
Kyrgyzstan
Kyrgyzstan
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Review
For citations:
Kasieva G.K. HISTOLOGICAL RESEARCH AND IMMUNE HISTOCHEMICAL TEST OF THE CATTLE LYMPH NODES . Bulletin of NSAU (Novosibirsk State Agrarian University). 2015;(1):101-106. (In Russ.)
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