Approaches to investigation of gene modified maize and semi-quantitaive analysis by means of СР4 EPSPS protein
Abstract
The article explores application of different methods aimed at finding gene-modified organisms (GMO) on concentration of protein in the samples of food products. As starting material, the researchers used seeds of gene-modified maize NK603 with different concentration of GMO represented by International Seed Testing Association (ISTA) in frames of the programme of quality assessment The researchers used specific test strips for qualitative and immunofluorescence assay (IFA) for semi-quantitative analysis where they used СР4 EPSPS molecules of protein. The research has shown that application of test stripes contributes to searching for 0.6 % of GMO. Sensation threshold of test stripes is found in temperature processing at 80 °C. The linear regressive correlation confirms ability to apply the suggested approach and immunofluorescence assay for semi-quantitative determination of GMO on protein СР4 EPSPS in the samples that contain GM-corn with concentration 0.1 %. The paper notes slight differences in application of filters with different waves.
About the Authors
З. Поповски
Университет св. Кирилла и Мефодия, Скопье, Македония
Russian Federation
Russian Federation
Э. Мискоска-Милевска
Университет св. Кирилла и Мефодия, Скопье, Македония
Russian Federation
Russian Federation
Т. Несторовски
Университет св. Кирилла и Мефодия, Скопье, Македония
Russian Federation
Russian Federation
Е. Камалдинов
Новосибирский государственный аграрный университет
Russian Federation
Russian Federation
В. Петухов
Новосибирский государственный аграрный университет
Russian Federation
Russian Federation
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, , , , Approaches to investigation of gene modified maize and semi-quantitaive analysis by means of СР4 EPSPS protein. Bulletin of NSAU (Novosibirsk State Agrarian University). 2016;(3):92-97. (In Russ.)
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