Application of Bacillus bacterial strains in the propagation of sea buckthorn by ligious cuttings

The studies were conducted in four-year production experiments in the nursery of the agricultural production cooperative (APC) “Sady Baraby” in the Novosibirsk region to assess the effectiveness of the Bacillus bacteria strains in obtaining sea buckthorn seedlings from lignified cuttings. A stimulating effect on the rooting of lignified sea buckthorn cuttings was proven in variants with pre-planting treatment with the strains B. subtilis VKPM B-10641, B. licheniformis VKPM B-10562 and a mixture of 3 strains (experimental biopreparation Fitop 8.67) in working concentrations of 1×105 CFU/ml an increase of 13.1-18.9 % relative to the control, to absolute levels of 48.8-51.3 %, acting at the same level of efficiency with the reference variant Heteroauxin. All the studied bacterial strains reliably stimulated the increase in seedling biomass by 9.1-20.5 % relative to the control, to a level of 19.0-20.4 g/plant. The most stable over 4 years of observation were the strain B. amyloliquefaciens VKPM B-10642 and a mixture of 3 strains, which can be stated to have the greatest growth-stimulating effect on sea buckthorn plants. An increase in the commercial quality of sea buckthorn seedlings was proven in the variants with pre-planting treatment with the strains B. amyloliquefaciens VKPM B-10642 and B. licheniformis VKPM B-10562, an increase of 28.6 % and 17.7 %, with 70.0 % in the control. The highest yield of seedlings, on average over 4 years, has been proven in variants with strains B. subtilis VKPM B-10641, B. licheniformis VKPM B-10562 and a mixture of 3 strains, which should be recognized as the most promising for use in obtaining seedlings from lignified cuttings of sea buckthorn.

1. Pomologija. Sibirskie sorta plodovyh i jagodnyh kul’tur HH stoletija (Pomology. Siberian varieties of fruit and berry crops of the 20th century), Novosibirsk: OOO «Jupiter», 2005, 568 p.

2. Ershova I.V., AgroNauka-2020 (AgroScience-2020), Proceedings of the international scientific online conference, Novosibirsk, 2020, pp. 36–39. (In Russ.)

3. Miroshnikov V.G., Osnovnye bolezni oblepihi i mery bor’by s nimi v uslovijah lesostepi Zapadngoj Sibiri (Main diseases of sea buckthorn and measures to combat them in the forest-steppe conditions of Western Siberia), Extended abstract of candidate’s thesis, Novosibirsk, 1996, 16 p.

4. Nebesnyh M.A., Vestnik IrGSHA, 2009, No. 37, pp. 15–21. (In Russ.)

5. Potapova A.V., Zubik I.N., Dorozhkina L.A., Materialy mezhdunarodnoj nauchnoj konferencii molodyh uchjonyh i specialistov, posvjashhjonnoj 150-letiju A.V. Leontovicha, Proceedings of the conference Title, Moscow, 2019, pp. 87–91. (In Russ.)

6. Gosudarstvennyj katalog pesticidov i agrohimikatov, razreshennyh k primeneniju na territorii Rossijskoj Federacii. Part I. Pesticidy (State catalog of pesticides and agrochemicals approved for use on the territory of the Russian Federation. Part I. Pesticides), Ministerstvo sel’skogo hozjajstva RF, Moscow, 2023, 945 p.

7. Rjabchinskaja T.A., Zimina T.V., Agrohimija, 2017, No. 12, pp. 62–92. (In Russ.)

8. Beljaev A.A., Shternshis M.V., Shpatova T.V., Lutov V.I., Leljak A.A., Leljak A.I., Judushkin V.V., Dostizhenija nauki i tehniki APK, 2014, No. 1, pp. 38–40. (In Russ.)

9. Beljaev A.A., Shternshis M.V., Shpatova T.V., Pospelova N.P., Leljak A.A., Leljak A.I., Lutov V.I., Dostizhenija nauki i tehniki APK, 2015, No. 4, pp. 31–34. (In Russ.)

10. Beljaev A.A. Shpatova T.V., Nevolin S.V., Gavrilec T.V., Vestnik NGAU (Novosibirskiy gosudarstvennyy agrarnyy universitet), 2024, No. 4 (73), pp. 12–20. (In Russ.)

11. Proizvodstvo i sertifikacija posadochnogo materiala plodovyh, godnyh kul’tur i vinograda v Rossii. Kontrol’ kachestva. Part 1. Jagodnye kul’tury (Production and certification of planting material for fruits, suitable crops and grapes in Russia. Quality control. Part 1. Berry crops), Moscow: VSTISP, 2009. 164 p. (In Russ.)

12. Programma i metodika sortoizucheniya plodovykh, yagodnykh i orekhoplodnykh kul’tur (Program and methodology for variety study of fruit, berry and nut crops), Orel, 1999, 606 p. (In Russ.)

13. Dospekhov B.A., Metodika polevogo opyta: s osnovami statisticheskoi obrabotki rezul’tatov issledovanii (Field experience methodology: with the basics of statistical processing of research results), Moscow, 2013, 349 p.

14. Sorokin O.D., Prikladnaya statistika na komp’yutere (Applied statistics on the computer), Krasnoobsk: GUP RPO SO RASKhN, 2009, 222 p.