Influence of succarose concentration on the growth and development of strawberry regenerants
https://doi.org/10.31677/2072-6724-2025-75-2-79-87
Abstract
The aim of the study was to optimize the stages of garden strawberry micropropagation. To introduce the Elizaveta 2 variety and the K10 selection line into in vitro culture, the growth points of the firstorder runners were used as explants using the following sterilization scheme: soap solution; running water; 70% ethanol; 12 % H2 O2 . At the stage of micropropagation itself, Murashige-Skoog medium was used with a sucrose concentration of 0, 10, 20, 30 g/l, supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The final stage of the work included determining the ability of the regenerates to adapt to ex vitro conditions. Vermiculite was used as a substrate. The experiment was repeated three times. The number of roots and microplants from the explant, as well as their viability at the adaptation stage, were recorded. The Elizaveta 2 variety and the K10 line did not have statistically significant differences in the increase in the number of microshoots. The Elizaveta 2 variety showed a tendency to decrease by 2.03 pcs. (LSD05 = 4.09) in the variant with the addition of 30 g/l sucrose, and the number of roots statistically significantly exceeded the value of the indicator on the control medium (20 g/l sucrose; LSD05 = 2.64) by 3.23 pcs. On the nutrient medium with the addition of 10 g/l sucrose, the number of roots decreased by 3.77 pcs., and on the medium without sucrose – by 8.38 pcs. The K10 selection line did not form roots in the variant without the addition of sucrose, and no statistically significant deviations were observed in the other variants. Under ex vitro conditions, the micro plants were characterized by 88.8–100 % viability after in vitro cultivation on variants with 20–30 g/l sucrose. Adapted micro plants, cultivated in vitro on a diet containing 30 g/l of sucrose, have a powerful root system (Elizaveta 2 by 6.42 pcs. more, line K10 by 4.23 pcs., LSD05 = 4.86), compared to the indicators in the control variant.
About the Authors
A. A. Poteshkina
Federal Research Center the Institution of Cytology and Genetics (Siberian Research Institute of Plant Production and Breeding – branch)
Russian Federation
Russian Federation
Junior Researcher
pos. Krasnoobsk, Novosibirskaya obl.
V. A. Aparina
Federal Research Center the Institution of Cytology and Genetics (Siberian Research Institute of Plant Production and Breeding – branch)
Russian Federation
Russian Federation
Junior Researcher
pos. Krasnoobsk, Novosibirskaya obl.
V. V. Piskarev
Federal Research Center the Institution of Cytology and Genetics (Siberian Research Institute of Plant Production and Breeding – branch)
Russian Federation
Russian Federation
PhD in Agricultural Sciences, Head of Laboratory
pos. Krasnoobsk, Novosibirskaya obl.
References
1. Akimov M.Yu., Luk’yanchuk I.V. Zhbanova E.V., Lyzhin A.S., Khimiya rastitel’nogo syr’ya, 2020, No. 1, pp. 5–18, DOI: 10.14258/jcprm.2020015511. (in Russ.)
2. Samtani J.B., Rom C.R., Friedrich H. et al.,The status and future of the strawberry industry in the United States, HortTechnology, 2019, Vol. 29, No. 1, pp. 11–24, DOI:10.21273/HORTTECH04135-18.
3. Huang M., Han Y., Li L., Rakariyatham K., Wu X., Gao Z., Xiao H., Protective effects of non-extractable phenolics from strawberry against inflammation and colon cancer in vitro, Food Chemistry, 2022, Vol. 374, pp. 131759, DOI: 10.1016/j.foodchem.2021.131759.
4. Kozlova I.I., Lukyanchuk I.V., Zhbanova E.V., Dostizheniya nauki i tekhniki APK, 2019, Vol. 33, No. 2, pp. 45–49, DOI: 10.24411/0235-2451-2019-10211. (in Russ.)
5. Aysanov T.S., Nauchnye trudy SKFNTsSVV, 2023, Vol. 37, No. 1, pp. 95–99, DOI: 10.30679/2587-9847-2023-37-95-99. (in Russ.)
6. Fránová J., Přibylová J., Koloniuk I., Molecular and biological characterization of a new strawberry cytorhabdovirus, Viruses, 2019, Vol. 11, No. 11, pp. 982, DOI: 10.3390/v11110982.
7. Matushkina O.V., Pronina I.N., Selektsiya i sortorazvedenie sadovykh kul’tur, 2019, Vol. 6, No. 1, pp. 74–77. (in Russ.)
8. Lamonova I.A., Verzilin A.V., Vestnik Michurinskogo gosudarstvennogo agrarnogo universiteta, 2015, No. 4, pp. 57–61. (in Russ.)
9. Puscan R., Castro E.R.V., Chanamé C.E.M., Combined effect of cytokinins on the in vitro propagation of three strawberry cultivars, Revista Caatinga, 2024, Vol. 37, pp. e12180–e12180, DOI: 10.1590/1983-21252024v3712180rc.
10. Sevcikova H., Lhotakova Z., Hamet J., Lipavska H., Mixotrophic in vitro cultivations: the way to go astray in plant physiology, Physiologia plantarum, 2019, Vol. 167, No. 3, pp. 365–377, DOI: 10.1111/ppl.12893.
11. Naser S.M., Abdulhussein M.A.A., Effect of sucrose in strawberry micro-propagation using platform bioreactor under temporary immersion system, Revista Bionatura, 2023, Vol. 4, pp. 1–7, DOI: 10.21931/RB/CSS/2023.08.04.94.
12. Kornatskiy S., The final stage of micropropagation of garden strawberries, Proceedings of the Conference Title AGRITECH-IX 2023, 2024, Vol. 486, pp. 01016, DOI: 10.1051/e3sconf/202448601016.
13. Ferrari M.P.S., Cruz R.M.S., Queiroz M.S., Andrade M.M., Alberton O., Magalhães H.M., Efficient ex vitro rooting, acclimatization, and cultivation of Curcuma longa L. from mycorrhizal fungi, Journal of Crop Science and Biotechnology, 2020, Vol. 23, pp. 469–482, DOI: 10.1007/s12892-020-00057-2.
14. Gusev D.A., Plaksina T.V., Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta, 2022, Vol. 9(215), pp. 31– 36, DOI: 10.53083/1996-4277-2022-215-9-31-36. (in Russ.)
15. Yartseva M.A., Khvostova A.B., Ivanova L.A., Slukovskaya M.V., Trudy Karel’skogo nauchnogo tsentra RAN, 2024, No. 7, pp. 91–101, DOI: 10.17076/eb1915. (in Russ.)
Review
For citations:
Poteshkina A.A., Aparina V.A., Piskarev V.V. Influence of succarose concentration on the growth and development of strawberry regenerants. Bulletin of NSAU (Novosibirsk State Agrarian University). 2025;(2):79-87. (In Russ.) https://doi.org/10.31677/2072-6724-2025-75-2-79-87
Views:
50
Email this article 
