Assessment of the source material of Clarkia Pursh by breeding valuable economic and biological characteristics in the conditions of the south Western Siberia

An assessment of the economic and biological value of Clarkia Pursh varieties of ornamental annual herbaceous plants is presented based on a set of characteristics important for different areas of breeding of this crop in the south of Western Siberia. The scheme of the experiment included 20 variants, in 4 repetitions. The experiment included 5 new domestic varieties created by the author on the basis of the Novosibirsk State Agrarian University (55°01’ s. 82°55’ w.d.). The first domestic variety is Clarkia purpurea (Curtis) A. Nelson & J.F. Macbr. The Purple Fairy was characterized by an early flowering period (42±1 day after germination), Cv = 4,0 %, a short growing season of 102±2 days, high seed productivity (1000 seeds = 0,73±0,004 and seeds from one plant = 5,5±0.02 g), and pollen viability – 82 %, fruit setting – 93 % and seed productivity coefficient (KSP) – 83 % and high 100 % laboratory germination of seeds. Among the clarkia samples, sources of five different flowering periods were identified: 15 % – early flowering; 2) 15 % – mid-early flowering; 3) 20 % – medium flowering; 4) 40 % – mid-late blooming; 5) 10 % – late blooming. For the first time in the climatic conditions of the south of Western Siberia, temperature limits for the passage of interphase periods were established for 3 Clarkia species according to the sum of active temperatures above 10°C: С. рurpurea required the smallest sum of active temperatures above 10°C (Q1=801°C – Q3 =1723°C), with an average value of 1234°C. The sources of the abundance of flowering have been identified. The author’s varieties were distinguished by high seed qualities. A direct dependence of the seed productivity coefficient on the viability of pollen has been established (r = 0.99, p < 0.05). The early clarkia varieties, C. purpurea (5.5 g) and C. unguiculata (3.8 g), had the highest seed productivity. Thus, a comprehensive assessment of Clarkia samples served as the basis for the development of the national methodology RTG/1157/1 for conducting tests on Clarkia Pursh environmental protection systems, made it possible to identify sources of selectively valuable economic and biological traits and create a fund of original seeds that provides directions for further breeding and reproduction of promising samples for their further implementation in the production and landscaping of the region.

1. Lewis H., The mechanism of evolution in the genus Clarkia, Evolution, 1953, Vol. 7, No. 1, pp. 1–20.

2. Lewis H., The Origin of Diploid Neospecies in Clarkia, The American Naturalist, 1973, Vol. 107, No. 954, pp. 161– 170, JSTOR, www.jstor.org/stable/2459792 (data obrashheniya: 10.04. 2025).

3. Lewis H., Clarkia Pursh, Flora of North America, URL: http://dev.floranorthamerica.org/Clarkia (data obrashheniya: 10.04. 2025).

4. Munz Ph., Keck D.A, California Flora, University of California Press. Berkeley Los Angeles, London, 1973, pp. 923–961.

5. Overson R.P. [et al.], A phylogeny of the evening primrose family (Onagraceae) using a target enrichment approach with 303 nuclear loci. BMC, Ecol Evol, 2023, Vol. 23, No. 66, pp. 1–16, DOI: 10.1186/s12862–023–02151–9, PMID: 37974080; PMCID: PMC10655384.

6. Wagner W.L., Hoch P.C., Raven P.H., Revised classification of the Onagraceae Systematic Botany Monographs, 2007, Vol. 83, pp. 1–240.

7. Koroleva E.V., Fotev Yu.V., Byulleten` Gosudarstvennogo Nikitskogo botanicheskogo sada, 2024, No. 151, pp. 18– 29. (In Russ.).

8. Koroleva E.V., Fotev Yu.V., Vestnik NGAU (Novosibirskij gosudarstvenny`j agrarny`j universitet), 2024, No. 3(72), pp. 44–64, DOI: 10.31677/2072-6724-2024-72-3-44-64. (In Russ.).

9. Koroleva E.V., Fotev Yu.V., Genofond i selekciya rastenij (Gene pool and plant breeding), Materialy` 7-j Mezhdunar. konf., posvyashhennoj 95-letiyu akademika RAN P.L. Goncharova, Novosibirsk, 10–12 apr. 2024 g., Novosibirsk: Federal`ny`j issledovatel`skij centr institut citologii i genetiki SO RAN, 2024, pp. 171–175, DOI: 10.18699/GPB2024-44. (In Russ.).

10. Koroleva E.V., Vestnik NGAU (Novosibirskij gosudarstvenny`j agrarny`j universitet), 2023, No. 2(67), pp. 60–74, DOI: 10.31677/2072-6724-2023-67-2-60-74. (In Russ.).

11. Sharma P., Gupta Y.C., Dhiman S.R. [et al.], Variation in growth, flowering and seed yield of satin flower (Godetia grandiflora) planted on different date, Indian Journal of Agricultural Sciences, 2016, Vol. 86, No. 2, pp. 277–280.

12. Svyatkovskaya E.A., Saltan N.V., Umanecz M.S., Agropromy`shlenny`e texnologii Central`noj Rossii, 2022, No. 2(24), pp. 61–70, DOI: 10.24888/2541-7835-2022-24-63-72. (In Russ.).

13. By`lov V.N., Osnovy` sravnitel`noj sortoocenki dekorativny`x rastenij, Introdukciya i selekciya czvetochnodekorativny`x rastenij (Introduction and selection of ornamental plants), Moscow: Nauka, 1978, pp. 7–32.

14. Goncharov N.P., Goncharov P.L., Metodicheskie osnovy` selekcii rastenij (Methodological principles of plant breeding), Novosibirsk, 2018, 439 p. (In Russ.).

15. Reut A.A., E`poxa nauki, 2017, No. 12, pp. 219–225, DOI: 10.1555/2409-3203-2017-0-12-219-225, EDN ZXRDNX. (In Russ.).

16. Raxmangulov R.S., Tixonova N.G., Biotexnologiya i selekciya rastenij, 2021, T. 4, No. 4, pp. 40–54, DOI: 10.30901/2658-6266-2021-4-o4.

17. Datta S.K., Breeding of ornamentals: success and technological status, Nucleus, 2022, Vol. 65, pp. 107–128, DOI: 10.1007/s13237-021-00368-x.

18. Bugallo V., Facciuto G., Selection process in ornamental plant breeding, Ornamental Horticulture, 2023, Vol. 29, pp. 68–75, DOI: 10.1590/2447-536x.v29i1.2617.

19. Bolgov V.I., Ivsyukova V., Kozina V.V. [i dr.], Metodika pervichnogo sortoizucheniya czvetochny`x kul`tur (Methodology of primary variety study of flower crops), Moscow, 1998. (In Russ.)

20. Metodika RTG/1157/1 provedeniya ispy`tanij na otlichimost`, odnorodnost`i stabil`nost` Klarkiya (Clarkia Pursh) (RTG/1157/1 Method for testing the distinctness, homogeneity and stability of Clarkia (Clarkia Pursh)), URL: https://gossortrf.ru/publication/metodiki-ispytaniy-na-oos.php (data obrashheniya: 10.04.2025). (In Russ.).

21. Bejdeman I.N., Metodika izucheniya fenologii rastenij i rastitel`ny`x soobshhestv (Methodology for studying the phenology of plants and plant communities), Novosibirsk: «Nauka», 1974, 154 p. (In Russ.).

22. GOST 24933.0–81, Semena czvetochny`x kul`tur. Metody` opredeleniya vsxozhesti i e`nergii prorastaniya semyan [E`lektronny`j resurs], URL: https://files.stroyinf.ru/Data/138/13865.pdf (data obrashheniya: 10.04.2025). (In Russ.).

23. GOST 12260–81, Semena odnoletnix i dvuxletnix czvetochny`x kul`tur. Posevny`e kachestva, Texnicheskie usloviya [E`lektronny`j resurs], URL: https://allgosts.ru/65/020/gost_12260-81 (data obrashheniya: 10.04.2025). (In Russ.).

24. Eremenko L.L., Introdukciya rastenij v Sibiri, Novosibirsk: Nauka. Sib. otd.-nie, 1977, pp. 212–226. (In Russ.).

25. Levina R.E., Reproduktivnaya biologiya semenny`x rastenij (Reproductive biology of seed plants), Moscow: Nauka, 1981, 72 p. (In Russ.).

26. Metodicheskie ukazaniya po semenovedeniyu introducentov (Methodical instructions for seed production of introduced species), Moscow: Nauka, 1980, 64 p.

27. Tkachenko K.G., Oxrana i racional`noe ispol`zovanie lesny`x resursov (Protection and rational use of forest resources), Mat-ly` X mezhdunar. foruma, Blagoveshhensk, 2019, pp. 194–197. (In Russ.).

28. Tkachenko K.G., Hortus Botanicus, 2020, T. 15, pp. 228–248. (In Russ.).

29. Tkachenko K.G., Rol` botanicheskix sadov v soxranenii i obogashhenii prirodnoj i kul`turnoj flory`(The role of botanical gardens in preserving and enriching natural and cultural flora), Mat-ly` Vseros. Konf. s mezhdunar. uchastiem, Yakutsk, 2021, pp. 42–48. (In Russ.)

30. Tkachenko K.G., Gryaznov A.Yu., Staroverov N.E., Sovremenny`e problemy` prirodopol`zovaniya, oxotovedeniya i zverovodstva (Modern problems of nature management, hunting and animal husbandry), Mat-ly` Mezhdunar. nauch.– prakt. konf., Kirov, 2022, pp. 330–333. (In Russ.).

31. Koroleva E.V., Fotev Yu.V., BIOAsia Altai 2024, Mat-ly` IV Mezhdunar. biotexnol. foruma, Barnaul, 2024, pp. 312– 316. (In Russ.).

32. Fotev Yu.V., Isxodny`j material dlya selekcii tomata s ustojchivost`yu k stressovy`m temperaturam i boleznyam (Source material for breeding tomato with resistance to stress temperatures and diseases), Moscow, 2001, pp. 224–239. (In Russ.).

33. Fotev Yu.V., Vestnik NGAU (Novosibirskij gosudarstvenny`j agrarny`j universitet), 2018, No. 4(49), pp. 104–118, DOI: 10.31677/2072-6724-2018-49-4-104-118. (In Russ.).

34. Fotev Yu.V., Trudy` po prikladnoj botanike, genetike i selekcii, 2022, No. 183(3), pp. 39–47, DOI: 10.30901/2227-8834-2022-3-39-47. (In Russ.).

35. Koroleva E.V., Problemy` botaniki Yuzhnoj Sibiri i Mongolii, 2022, No. 21-2, pp. 69–75, DOI: 10.14258/ pbssm.2022057, EDN JCYTXC. (In Russ.).

36. Vereshhagina I.V., Kul`tura czvetochny`x rastenij v Altajskom krae (Flowering Plant Culture in the Altai Region), Barnaul, 1968, 142 p. (In Russ.).

37. Pas`ko O.A., E`kologicheskie aspekty` povy`sheniya produktivnosti czvetochny`x, ovoshhny`x kul`tur i kartofelya v taezhnoj zone Zapadnoj Sibiri (Ecological aspects of increasing the productivity of flower, vegetable crops and potatoes in the taiga zone of Western Siberia), Candidate’s abstract, Novosibirsk, 2000, 32 p.

38. Bao-Ming Chen, D’Antonio C.M., Molinari N. [et al.], Mechanisms of influence of invasive grass litter on germination and growth of coexisting species in California, Springer International Publishing AG, part of Springer Nature, 2017, Published online: 18 January 2018.