ESTIMATION OF NEKROFARM 20 PREPARATION ACUTE TOXICITY

Abstract

The experiment was conducted to determine acute toxicity of the generic preparation Nekrofarm 20, which active substance is oxytetracycline dehydrate. The test was carried out on laboratory animals, 50 pedigreeless white mice were used, which were grouped into 4 experimental and 1 control with the method of random numbers. Gastric tube was used for one-time introduction of the preparation into mice at the doses 3600, 3000, 1800, 1200 mg/kg, which corresponds to 0.3; 0.25; 0.15 and 0.1 ml per 10 g of animal body weight. The toxic effect of the high dosed preparation on the laboratory animals was followed by typical intoxication and death of some of the experimental animals. The testing of the preparation Nekrofarm 20 showed that MPD made up 1200 mg/kg, LD100 - 3600, LD50 - 2190 mg/kg when calculated with Kreber method. Agreed to the standard hygiene classification the preparation is of moderate toxicity for laboratory animals and refers to the substances of class III danger according to GOST (State Standard) 12.1.007-76.