Toxicological studies of additives feed endoforce multi on laboratory animals

The creation of new drugs and feed additives is an urgent task that contributes to the growth of the efficiency of the livestock and poultry industries of agriculture, especially during the period of import substitution in the Russian Federation. An experimental study of possible changes in the body of laboratory animals after single or multiple uses of new feed additives is necessary for the toxicological assessment of newly created products. Single intragastric administration of feed additive Endoforce Multi containing live vegetative cells and spores of cultures: Bacillus subtilis B-5449, Bacillus subtilis B-1323, Bacillus subtilis B-3679 - 5.0 109 CFU / ml, at a dose many times higher than recommended, did not lead to the death of laboratory animals, which confirmed the absence of its acute toxicity. Over the entire observation period, the authors did not note any disturbances in the rats’ behavioural reactions and physiological states. Determination of chronic toxicity was carried out for 30 days. The studied suspension of the feed additive was given with water to rats of the experimental groups at doses: 1st - 100 mg, 2nd - 150 mg and 3rd - 200 mg per head. At the end of the empirical study, an increase in the number of erythrocytes by 2.68–3.72 1012/l, haemoglobin - by 41.50–61.58 g/l in laboratory animals of the experimental groups compared with the control ones was revealed, which indicated an increase in blood oxygenation and the organism as a whole. In addition, in the rats of the experimental groups, a decrease in the content of urea in the blood to 3.14 ± 0.53 - 4.68 ± 0.41 mmol / l and creatinine to 32.26 ± 5.18 - 48.13 ± 6.23 µmol/l. The opposite trend was observed when determining the serum activity of alkaline phosphatase, which increased by 39.0–52.6% in intact individuals, which indicated dynamic processes of growth, formation and development of the skeleton. According to the results of the autopsy of the rats of the studied groups, regardless of the dose of the introduction of the feed additive Endoforce Multi, no pathological changes in the colour, consistency and size of the internal organs were registered.

1. Bakulina L.F., Timofeev I.V., Perminova N.G., Polushkina A.F., Pechorkina N.I., Biotekhnologiya, 2001, No. 2, pр. 48–56. (In Russ.)

2. Osipova I.G., Sorokulova I.B., Vasil’eva E.A., Mikrobiologiya, epidemiologiya i immunobiologiya, 2003, No. 3, pр. 113–119. (In Russ.)

3. Pokhilenko V.D., Perelygin V.V., Khimicheskaya i biologicheskaya bezopasnost’, 2007, No. 32– 33 (2–3), pр. 20–41. (In Russ.)

4. Akchurin S.V., Aktual’nye voprosy veterinarnoi biologii, 2013, No. 2 (18), pр. 13–16. (In Russ.)

5. Ulit’ko V.E. Lifanova S.P., Erisanova O.E., Vestnik Ul’yanovskoi gosudarstvennoi sel’skokhozyaistvennoi akademii, 2019, No. 2 (46), pр. 197–200. (In Russ.)

6. Dezhatkina S.V., Zyalalov Sh.R., Mukhitov A.Z., Agrarnaya nauka, 2021, No. 2, pр. 45–49. (In Russ.)

7. Dezhatkina S.V., Zyalalov Sh.R., Dezhatkin M.E., Vestnik Ul’yanovskoi gosudarstvennoi sel’skokhozyaistvennoi akademii, 2021, No. 1 (53), pр. 170–174. (In Russ.)

8. Zyalalov Sh.R., Il’inskaya M.A., Sharonina N.V., Dezhatkina S.V., Mukhitov A.Z., Uchenye zapiski Kazanskoi gosudarstvennoi akademii veterinarnoi meditsiny im. N.E. Baumana, 2021, Vol. 246, No. 2, pр. 88–93. (In Russ.)

9. Romanova Yu.A., Dezhatkin I.M., Dezhatkina S.V., Akhmetova V.V., Problemy i puti razvitiya veterinarnoi i zootekhnicheskoi nauk, (Problems and Ways of Development of Veterinary and Zootechnical Sciences), Conference materials, Saratov, 2021, pр. 762–768. (In Russ.)

10. Zapadnyuk I.P., Zapadnyuk V.I., Zakhariya E.A., Zapadnyuk B.V., Laboratornye zhivotnye. Razvedenie, soderzhanie, ispol’zovanie v eksperimente (Laboratory animals. Breeding, maintenance, use in the experiment), Kyiv: Vishcha shkola, 1983, 383 p.

11. Rukovodstvo po eksperimental’nomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv (Guidelines for the experimental (preclinical) study of new pharmacological substances), Moscow: Meditsina, 2005, 832 p.

12. GOST 32644-2014 Metody ispytaniya po vozdeistviyu khimicheskoi produktsii na organizm cheloveka (Test methods for the effects of chemical products on the human body). (In Russ.)

13. GOST 33216-2014 Rukovodstvo po soderzhaniyu i ukhodu za laboratornymi zhivotnymi. Pravila soderzhaniya i ukhoda za laboratornymi gryzunami i krolikami (Guidelines for the maintenance and care of laboratory animals. Rules for the maintenance and care of laboratory rodents and rabbits). (In Russ.)

14. GOST 33215-2014 Rukovodstvo po soderzhaniyu i ukhodu za laboratornymi zhivotnymi. Pravila oborudovaniya pomeshchenii i organizatsii protsedur (Guidelines for the maintenance and care of laboratory animals. Rules for equipping premises and organizing procedures). (In Russ.)

15. GOST 34566-2019 Kombikorma polnoratsionnye dlya laboratornykh zhivotnykh. Tekhnicheskie usloviya (Complete feed for laboratory animals. Specifications). (In Russ.)

16. GOST R 51232-98 Voda pit’evaya. Obshchie trebovaniya k organizatsii i metodam kontrolya kachestva (Drinking water. General requirements for the organization and methods of quality control). (In Russ.)

17. Donnik I.M., Shkuratova I.A., Vestnik Rossiiskoi akademii nauk, 2017, Vol. 87, No. 4, pр. 362– 366. (In Russ.)

18. Lomako V.V., Problemy kriobiologii i kriomeditsiny, 2018, No. 28 (4), pр. 293–310. (In Russ.)

19. Ostrovskii V.K., Mashchenko A.V., Yangolenko D.V., Klinicheskaya laboratornaya diagnostika, 2006, No. 6, pр. 128–132. (In Russ.)

20. Sidel’nikova V.I., Chernitskii A.E., Retskii M.I., Sel’skokhozyaistvennaya biologiya, 2015, Vol. 50, No. 2, pр. 152–161. (In Russ.)

21. Kharlap S.Yu., Derkho M.A., Sereda T.I., Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta, 2015, No. 2 (52), pр. 103–105. (In Russ.)