Production of a biotechnological product on a nutritional medium from plant raw materials
https://doi.org/10.31677/2072-6724-2022-64-3-76-82
Abstract
Yeast production is one of the cost-effective ways to solve the problem of protein deficiency in food and feed. They are grown on nutrient media of various origins and compositions. Perennial non-traditional plants are promising in this regard. One of the industrial crops widely used in biotechnology, agriculture, medicine, food industry, etc., is Jerusalem artichoke (Helianthus tuberosus), including the variety Interest. The authors determined the chemical composition of Jerusalem artichoke tubers in the air-dry state. Data analysis showed that Jerusalem artichoke tubers have a rich composition and are a suitable raw material for nutrient media preparation. The nutrient medium was prepared by heat treatment. Yeast cultivation was carried out at a temperature of 37°C, pH 4.5. The yeast strain Saccharomyces cerevisiae was used as a producer of unicellular protein. The main component of the nutrient medium is Jerusalem artichoke. During cultivation, the dynamics of changes in the number of cells and accumulation of their biomass were studied. The increase in yeast biomass was 21 g/l, and the number of CFU was 450 million/ml. Active assimilation of sugars by yeast occurs from the first hour after the start of cultivation and stops by the ninth hour. Incomplete assimilation of sugars is associated with the accumulation of metabolic products in the environment, which significantly affects their activity. The obtained research results indicate the balance of the substrate in terms of nutritional components, as well as the expediency of its use for the cultivation of different types of yeast.
About the Authors
V. B. Tsugkieva
Gorsky State Agrarian University
Russian Federation
Russian Federation
Doctor of Agricultural Sciences, Professor
Vladikavkaz
L. B. Dzantieva
Gorsky State Agrarian University
Russian Federation
Russian Federation
Ph.D. in Biological Sciences, Associate Professor
Vladikavkaz
L. Kh. Tokhtieva
Gorsky State Agrarian University
Russian Federation
Russian Federation
Ph.D. in Biological Sciences, Associate Professor
Vladikavkaz
Dz. N. Doev
Gorsky State Agrarian University
Russian Federation
Russian Federation
Ph.D. in Biological Sciences, Associate Professor
Vladikavkaz
I. A. Shabanova
Gorsky State Agrarian University
Russian Federation
Russian Federation
Ph.D. in Agricultural Sciences, Associate Professor
Vladikavkaz
B. A. Datieva
Gorsky State Agrarian University
Russian Federation
Russian Federation
Senior Lecturer
Vladikavkaz
References
1. Neiman B.Ya., Industrija mikrobov (The microbial industry), Moscow: Knowledge: 1983. p. 208.
2. Vinnichenko A.N., Dvoretsky A.I., Biopreparaty v zhivotnovodstve i rastenievodstve (Biological products in livestock and crop production), Dnepropetrovsk: Promin, 1989. p. 124–125.
3. Bergkvist R., Yare N., Moder. Kemi, 1969, Vol. 11, pp. 44.
4. Bosenko A.M., Petrochenko L.V., Gidroliznaja i lesohimicheskaja promyshlennost’, 1977, No. 1, pp. 5–6. (In Russ.)
5. Andreev A.A., Bryzgalova L.I., Proizvodstvo kormovyh drozhzhej (Feed yeast production), Moscow: Forest industry, 1986, 248 p.
6. Paramonova I.E., Kravchenko N.A., Balpanov B.S., Ten O.A., Paramonova I.E., Biotehnologija, teorija i praktika, 2013, No. 1, pp. 52–56. (In Russ.)
7. Rodriguez V.I., Lapteva E.A., Borisenko E.G., Pishhevaja promyshlennost’, 2019, No. 5, pp. 49–51. (In Russ.)
8. Bannitsyna T.E., Kanarsky A.V., Shcherbakov A.V., Chebotar V.K., Kiprushkina E.I., Vestnik Mezhdunarodnoj akademii holoda, 2016, No. 1, pp. 24–29. (In Russ.)
9. Malashenko Yu.R., Sokolov I.G., Romanovskaya V.A., Mikrobnyj metabolizm ne rostovyh substratov (Microbial metabolism of non-growth substrates), Kyiv: Naukova Dumka, 1987, 189 p.
10. Tmenov I.D., Tsugkiev B.G., Ispol’zovanie produktov mikrobiologicheskogo sinteza v zhivotnovodstve (The use of products of microbiological synthesis in animal husbandry), Vladikavkaz: Era, 1996, 122 p.
11. Dzhanaev K.I., Biokonversija zelenoj massy i klubnej topinambura sorta Skorospelka raznymi vidami drozhzhej s cel’ju poluchenija kormovogo belka (Bioconversion of green mass and tubers of Jerusalem artichoke variety Skorospelka with different types of yeast in order to obtain feed protein), Ph. D. dis. ... cand. biologist. Sciences, Vladikavkaz, 2012, 28 p.
12. Medvedev V.V., Topinambur i topinsolnechnik – problemy vozdelyvanija i ispol’zovanija (Jerusalem artichoke and Jerusalem artichoke - problems of cultivation and use), abstracts of the III All-Union Scientific and Production Conference, 1991, 7-11 October, Odessa: Lighthouse, 1991, pp. 17. (In Russ.)
13. Tsugkieva V.B., Tsugkiev B.G., Dzantieva L.B., Kokoeva A.T., Tokhtieva L.Kh., Shabanova I.A., Tokhtieva E.A., Effect of feeding Yeast obtained from Sakhalin Buckwheat on the growth of broiler chickens, Jornal of Livestock Science, Vol. 12, pp. 71-75.
14. Tsugkiev B.G., Tsugkiev V.B., Khoziev A.M., Dzantieva L.B., Soldatova I.E., Biotehnologija, 2021, No. 37 (4), pp. 60–64. (In Russ.).
15. Tsugkieva V.B., Dzantieva L.B., Tsugkieva I.B., Novye napravlenija v reshenii problem APK na osnove sovremennyh resursosberegajushhih, innovacionnyh tehnologij (New directions in solving the problems of the agro-industrial complex based on modem resource-saving, innovative technologies), materials of the International scientific and practical conference dedicated to the 65th anniversary of victory in the Great Patriotic War. January 26-28, Volgograd, 2010, T. 2, pp. 164–166. (In Russ.)
16. Tsugkieva V.B., Hoziev A.M., Tsugkiev B.G., Dzantieva L.B., Khairbekov S.U., Pishhevaja promyshlennost’, 2021, No. 9, pp. 58–60. (In Russ.)
17. Tsugkiev B.G., Karkusova N.N., Hoziev A.M., Izvestija Gorskogo gosudarstvennogo agrarnogo universiteta, 2014, No. 51 (1), pp. 255–259. (In Russ.)
18. Pat. 2370531, Russian Federation, Tsugkiev B.G., Kabulova M.Yu., Tsugkieva I.B., Gekiev Z.A., dec. 16.06.2008; publ.20.10.2009. Bull. No. 29. (In Russ.)
19. Pat. 2449012, Russian Federation, Tsugkiev B.G., Kozyreva I.I., Ramonova E.V., Khaev D.L., dec. 17.05.2010; publ.27.04.2012. Bull. No. 12. (In Russ.)
20. Hoziev A.M., Tsugkiev B.G., Kozyrev S.G., Tsugkieva V.B., Siukaev S.A., Izvestija Gorskogo gosudarstvennogo agrarnogo universiteta, 2020, No. 57 (2), pp. 139–145. (In Russ.)
21. Babyeva I.P., Golubev V.I., Metody vydelenija i identifikacii drozhzhej (The Methods of yeast isolation and identification), Moscow: Food Industry, 1979, 120 p.
22. Patent No. 2445359 C2, Russian Federation, Tsugkiev B.G., Ramonova E.V., Gudiev R.V., application 17.05.2010, publ. 20.03.2012. (In Russ.)
Review
For citations:
Tsugkieva V.B., Dzantieva L.B., Tokhtieva L.Kh., Doev D.N., Shabanova I.A., Datieva B.A. Production of a biotechnological product on a nutritional medium from plant raw materials. Bulletin of NSAU (Novosibirsk State Agrarian University). 2022;(3):76-82. (In Russ.) https://doi.org/10.31677/2072-6724-2022-64-3-76-82
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