SOMATIC CHROMOSOMAL INSTABILITY IN HOLSTEIN COWS IN KUZBASS CONDITIONS
https://doi.org/10.31677/2072-6724-2020-56-3-123-130
Abstract
The results of studies of somatic chromosomal instability aneuploidy and polyploidy, the number of fragments and breaks in the blood cells of Holstein cows with a productivity of over 9000 kg are presented. The studies were carried out at JSC “Vaganovo” of the Promyshlennovskiy district of the Kemerovo Region on a population of Holstein cows. Sample preparation was carried out according to the method of P. Moorhead and co-authors, and their staining according to RomanovskyGiemsa. 2452 metaphase plates of cattle blood were studied. The ecological situation was analyzed in the zone of its keeping and breeding. Studies of soil, feed, organs and tissues in farm animals of various species confirm the fact that there are no heavy metal contamination on the territory of Western Siberia and their level is within the limits of sanitary standards. As a result of the study, the frequency of somatic chromosomal instability, including numerical violations and structural aberrations of chromosomes, was established. In the studied sample, the frequency of polyploidy was 0.74%, tetraploidy, triploidy and hexaploidy 0.41; 0.25 and 0.08%, respectively. The frequency of fragments and breaks was approximately the same - 3.83 and 2.91%, respectively. The overall level of chromosome aberrations was 6.75%. The number of diploid cells was 84.25%. Due to the paucity of data on the somatic chromosomal instability of the Holstein breed in Kuzbass conditions, the data obtained can be preliminarily considered as normal values, and also used in assessing the interior of animals.
About the Author
D. V. Samsonov
Novosibirsk State Agrarian University
Russian Federation
PhD Student
Russian Federation
PhD Student
References
1. Semenov A.S. Tsitogeneticheskii skrining v razlichnykh populyatsiyakh golshtinizirovannogo skota (Cytogenetic screening in different populations of Holstein cattle), Doctors thesis, Perm, 2009, 221 p.
2. Kulikova S.G. Tsitogeneticheskii monitoring krupnogo rogatogo skota v raznykh ekologicheskikh zonakh Zapadnoi Sibiri i Severnogo Kazakhstana (Cytogenetic monitoring of cattle in different ecological zones of Western Siberia and Northern Kazakhstan), Doctors thesis, Novosibirsk, 1998, 294 p.
3. Narozhvykh K.N., Konovalova T.V., Petukhov V.L., Syso A.I., Sebezhko O.I., Shinin N.I., Fedaev J.I., Korotkevich O.S., Kamaldinov E.V., Osadchuk L.V. Cadmium accumulation in soil, fodder, grain, organs and muscle tissue of cattle in West Siberia, International Journal of Advanced Biotechnology and Research, 2016, Vol. 7, No. 4, pp. 1758–1764.
4. Konovalova T.V., Narozhnykh K.N., Petukhov V.L., Fedyaev Y.I., Shishin V.I., Sebezhko O.I., Korotkevich O.S., Kamaldinov E.V., Osadchuk L.V. Cooper content in hair, bristle and feather in different species reared in Western Siberia, Journal of Trace Elements in Medicine and Biology, 2017, Vol. 44, No. 5, p. 74.
5. Osadchuk L.V., Kleshnev M.A., Sebezhko O.I., Korotkevich O.S., Shinin N.I., Konovalova T.V., Narozhnyh K.N., Petukhov V.L. Characterizing physiological status in three breeds of bulls reared under ecological and climate conditions of the Altai, Iraqi Journal of Veterinary Sciences, 2017, Vol. 31, No. 1, pp. 35–42.
6. Syso A.I., Lebedeva M.A., Cherevko A.S., Petukhov V.L., Sebezhko O.I., Konovalova T.V., Korotkevich O.S., Narozhnykh K.N., Kamaldinov E.V., Sokolov V.A. Ecological and biogeochemical evaluation of elements content in soils and fodder grasses of the agricultural lands of Siberia, Journal of Pharmaceutical Sciences and Research, 2017, Vol. 9, No. 4, pp. 368–374.
7. Sebezhko O.I., Petukhov V.L., Shishin N.I., Korotkevich O.S., Konovalova T.V., Narozhnykh K.N., Zheltikov A.I., Marenkov V.G., Nezavitin A.G., Osadchuk L.V., Chysyma R.B., Kuzmina E.E. Influence of anthropogenic pollution on interior parameters, accumulation on hevy metals in organs and tissues, and resistance to disorders in the yak population in the republic of Tyva, Journal of Pharmaceutical Sciences and Research, 2017, Vol. 9, No. 9, pp. 1530–1535.
8. Kachura V.S. Tsitologiya i genetika, 1982, Vol. 16, No. 4, pp. 60–71. (In Russ.).
9. Kushnir A.V., Glazko V.I., Petukhov V.L., Dimov G., Storzhuk S.I. Biologiya, genetika i selektsiya ovtsy (Biology, genetics and selection of sheep), Novosibirsk, NSAU, ITsiG, 2010, 524 p.
10. Andreeva V.A., Li V., Lyu M., Konovalova T.V., Klimanova E.A., Sebezhko O.I., Nazarenko A.V. Vestnik NSAU, 2019, No. 1 (53), рр. 23–31 (In Russ.).
11. Petukhov V.L., Ernst L.K., Gudilin I.I., Golubev A.K., Zlochevskaya K.V., Krasota V.F., Levatin D.L., Sorokina I.I., Trut L.N., Tsalitis A.A., Chamuha M.D., Borodin P.M., Diasov G.I. Geneticheskie osnovy selektsii (Genetic basis of selection), Moscow: Agropromizdat, 1983, 448 p.
12. Kochneva M.L. Monitoring populyatsii sel’skokhozyaistvennykh zhivotnykh v raznykh ekologicheskikh usloviyakh (Monitoring of populations of farm animals in different environmental conditions), Doctor’s thesis, Novosibirsk, 2005, 292 p.
13. Kochneva M.L., Buteeva S.K., Zhidenova A.N. Biotekhnologiya: sostoyanie i perspektivy razvitiya (Bacteriology: state and development prospects) Proceeding of the VIII Moscow International Congress. ZAO «Ekspo-biokhim-tekhnologii», RKhTUim. D.I. Mendeleeva, March 17–20, 2015, Moscow, 2015, pp. 220–222. (In Russ.).
14. Samsonov D.V. Aktual’nye problemy sel’skogo khozyaystva gornykh territoriy (Actual problems of mountain agriculture), Proceeding of the VII Scientific conferencededicated to the 70th anniversary of Gorno-Altai State University, June 06–09, 2019, Gorno-Altaisk, 2019, pp. 192–194. (In Russ.)
15. Samsonov D.V. Teoriya i praktika sovremennoy agrarnoy nauki (Theory and practice of modern agricultural science), Proceeding of 2nd All-Russia Conference, February 26, 2019, Novosibirsk, 2019, pp. 346–348. (In Russ.)
16. Dzіtsyuk V.V., Peredrіi M.M. Bіologіya tvarin, 2017, Vol. 19, No. 3, pp. 36–41.
17. Kamaldinov E.V., Korotkevich O.S., Petukhov V.L. Selskokhozyaistvennaya biologiya, 2011, Vol. 46, No. 2, pp. 51–56. (In Russ.).
18. Kamaldinov E.V., Sebezhko O.I. Agrarnaya nauka – sel’skokhozyaistvennomu proizvodstvu Sibiri, Kazakhstana, Mongolii, Belarusii Bolgarii (Agricultural science – agricultural production in Siberia, Kazakhstan, Mongolia, Belarus and Bulgaria), Proceeding of theXX International Practical Conference, October 04–06, 2017, Novosibirsk, 2017, pp. 191–194.
19. Chysyma R.B. Khozyaistvenno-biologicheskie osobennosti yakov v razlichnykh usloviyakh Respubliki Tyva (Economic and biological characteristics of yaks in different conditions of the Tyva Republic), Doctors thesis, Novosibirsk, 2006, 252 p.
20. Chysyma R.B., Kochneva M.L., Petukhov V.L. Sibirskii vestnik sel’skokhozyaistvennoi nauki, 2005, No. 1, p. 110–112. (In Russ).
21. Moorhead P.S., Nowell P.C., Mellman W.J., Battlps D.M., Hunge-jord D.A. Chromosome preparations of leucocytes cultured from human peripheral blood, Exptl Cell Res., 1960, Vol. 20, No. 3, pp. 613–616.
22. Bochkov N.P., Kozlov V.M., Sevankaev A.V., Antoshchina M.M. Genetika,1966, No.10, pp. 120–124 (In Russ.).
23. Skiba T.V., Tsygankova A.R., Borisova N.S., Narozhnykh K.N., Konovalova T.V., Sebezhko O.I., Korotkevich O.S., Petukhov V.L., Osadchuk L.V. Direct determination of cooper, lead and cadmium in the whole bovine blood using thick film modified graphite electrodes, Journal of Pharmaceutical Sciences and Research, 2017, Vol. 9, No. 6, pp. 958–964.
24. Kamaldinov E.V., KushnirA.V., Petukhov V.L., Korotkevich O.S. Sibirskii vestnik sel’skokhozyaistvennoi nauki, 2010, No. 12 (216), pp. 67–73 (In Russ).
25. Konovalova T.V. Kormoproizvodstvo, produktivnost», dolgoletie i blagopoluchie zhivotnykh (Feed production, productivity, longevity and animal welfare), Proceeding of the International Scientific conference, October 04–05, 2018, Novosibirsk, 2018, pp. 28–30. (In Russ.)
26. Kulikova S.G. Sovremennye problem nauki i obrazovaniya, 2015, No. 3, p. 584 (In Russ).
27. Andreeva V.A. Ekologicheskie problemy zhivotnykh i cheloveka (Environmental problems of animals and humans), Proceeding of the conference, Novosibirsk, 2019, pp. 31–34. (In Russ.)
28. Andreeva V.A., Saurbaeva R.T. Sostoyanie i perspektivy uvelicheniya proizvodstva vysokokachestvennoy produktsii sel’skogo khozyaystva (Status and prospects of increasing the production of high-quality agricultural products), Proceedings of the VII International Scientific and Practical Conference, Novosibirsk, 2019, рр. 4–6.
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For citations:
Samsonov D.V. SOMATIC CHROMOSOMAL INSTABILITY IN HOLSTEIN COWS IN KUZBASS CONDITIONS. Bulletin of NSAU (Novosibirsk State Agrarian University). 2020;(3):123-130. (In Russ.) https://doi.org/10.31677/2072-6724-2020-56-3-123-130
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