NFLUENCE OF ETHANOLIC EXTRACT OF BEE-MOTH (GALLERIA MELLONELLA) ON THE ORGANS OF MICE

The paper highlights the effect of 40% alcohol extract of large bee- moth (Galleria mellonella) on the organs of mice. The researchers used white mice, arranged in groups of 5. The control group consisted of mice that did not receive the extract. The researchers applied the specimen in mice of groups 1, 2 and 3, when the specimen was applied during 21 days dozed 6, 12, 24 ml per 100 ml (0.3, 0.6 and 0.9%), respectively. The authors found out that in histological examination of the myocardium in all experimental groups, the structure of the endocardium, myocardium and epicardium corresponded to physiological parameters. The authors observed the tendency to increase the size of renal bodies and a slight increase in urinary space in the kidney tissues of all experimental animals. This may indicate some increase in renal filtration. The authors observed less thickness of the glomerular zone of the adrenal cortex in the animals of the 2nd and 3rd experimental groups. Higher thickness of the beam zone was observed only in animals of the 1st experimental group, which could indicate stress effects. This lower index was observed in other experimental groups of mice, which could be caused by depletion of compensatory mechanisms. After applying the extract dozed 0.3 %, the authors found out an increase in the thickness of the adrenal brain zone, while 0.6 and 0.9% dozes led to the decrease of the index. Changes in the liver certify the impact of ethyl alcohol on hepatocytes. The authors observed higher number of dual-core hepatocytes and vacuolazed ones in proportion to higher concentration of the extract in the experimental animals. Studying the spleen, the researchers found an increase in the area of the white pulp and signs of blast transformation in the animals of the 2nd experimental group. Thus, oral application of G. mellonella extract in different dosage provokes histological changes that characterize compensatory reactions, severity of which was determined by specimen doze. The authors didn’t prove specimen toxicity. The specimen dozed 0.3 % has a frank adaptogenic effect on the internal organs of mice.

extract of bee-moth, parenchymal organ, supramental capsule, compensatory reaction,, adaptogene, histology

1. Rachkov A. K., Rachkova M. A. Apiterapiya: Posobie dlya vrachej (Apitherapy: A Manual for Physicians), Ryazan», 2003, 250 p.

2. Spiridonov N. A., Rachkov A. K., Kondrashova M. N., Pchelovodstvo, 1993, No. 4, pp. 5–8. (In Russ.)

3. Ludyanskij F. A. Apiterapiya (Apitherapy), Vologda: Polifehst, 1994, 456 p.

4. Karneev F. D., Pchelovodstvo, 1999, No 4, pp. 55–56. (In Russ.)

5. Reuckij I. A. Lechenie medom i drugimi produktami pchelovodstva: Rekomendacii dlya vrachej i pacientov (Treatment with honey and other beekeeping products: Recommendations for doctors and patients), Moscow.: EHKSMO, 2007, 448 p.

6. Kolosova S. F., Pimenova A. O., Kashkarova I. V. Sostoyanie i perspektivy razvitiya pishchevoj promyshlennosti i obshchestvennogo pitaniya (The state and prospects of the development of the food industry and public catering), Proceedings of the 5th International Scientific and Practical Conference, Chelyabinsk, 2011, pp. 157–160. (In Russ.)

7. Solodenko YU.N., Solodenko I. V. Bol’shaya voskovaya mol» (Large wax moth), Odessa: VMV, 2012, 24 p.

8. Rachkov A. K., Rachkova M. A., Cygankova A. I. Pererabotka biologicheski aktivnyh produktov pchelovodstva i ih ispol’zovanie (Processing of biologically active beekeeping products and their use) Proceedings of the All-Russian Scientific and Practical Conference, June 25–27, Moskva, 1997, pp. 53. (In Russ.)

9. Kondrashova M. N., Litvinova E. G., Ovsepyan A. A., Mubarakshina EH.K. Fundamental’nye nauki – medicine (Fundamental sciences – medicine), Proceedings of the Conference Title, December 14–16, Moskva, Slovo, 2005, pp. 33–35. (In Russ.)

10. Sidorov P. I. Ispol’zovanie laboratornyh zhivotnyh v toksikologicheskom ehksperimente (The use of laboratory animals in a toxicological experiment), Arhangel’sk, 2002, 15 p.

11. Avtandilov G. G. Medicinskaya morfometriya (Medical morphometry), Moskva: Medicina, 1990, 334 p.

12. Plohinskij N. A. Rukovodstvo po biometrii dlya zootekhnikov (Biometrics manual for livestock experts, Moscow.: Kolos, 1969, 256 p.

13. Merkur’eva E. K. Biometriya v selekcii i genetike sel’skohozyajstvennyh zhivotnyh (Biometrics in the selection and genetics of farm animals), Moscow: Kolos, 1970, 424 p.

14. Lakin G. F. Biometriya (Biometrics), Moscow: Vyssh. shk., 1990, 352 p.