STANDARD PLANTING STOCK OF SWEET-BERRY HONEYSUCKLE APPLYING   BIOTECHNOLOGICAL METHODS 

The paper highlight efficiency of standard nursery transplant producing of blue honeysuckle on the basis of recovering and accelerated propagation. This process lasts 15-16 months and it is divided into the following stages: introduction in culture in vitro, micro-propagation (proliferation) itself, rooting of microsprouts, adaptation to non-sterile conditions in the laboratory and completion of growing in the open ground. When introduced into sterile environment, growing medium Woodi Plant Medium, in comparison with traditional MurashigeSkoog medium, provided significant increase in the survival rate of explants which was 62.2 %. At the stage of micropropagation, appropriate spectral composition for microsprouts of sweet-berry honeysuckle was provided by light-emitting diode radiator with a combination of red, blue and white light 2 : 1: 1, respectively. Modified Murashige-Skoog medium appeared to be the most effective at this stage. When researchers added 1.0 mg/l 6- BAP and 0.5 mg/l kinetin, it increased significantly propagation coefficient of microsprouts appropriate for rooting. At the rooting stage, light-emitting diode radiator with combination of red, blue and white light 2 : 1 : 1, respectively provided significant increase in rooting ability of microsprouts up to 89.0%, in comparison with control group (76.0%). At the stage of adaptation, application of growing medium on the basis of highbog peat and post-planting spraying with HB-10 increased microplants establishment. These adaptation conditions have significantly increased the number of sweet-berry honeysuckle established plants. Completion of growing of established meristem sweet-berry honeysuckle plants in containers when applying growing medium on the basis of highbog peat and lowland peat (1:1) increased the yield of standard annual planting stock up to до 94.0 %. The suggested advanced biotechnological techniques that consist of five stages, allow to increase significantly the yield of the standard planting stock of sweet-berry honeysuckle in 5.5 times, to reduce the cost of planting stock on 15.2 %. The standard planting stock with closed root system was reduced to one vegetation period.

1. Il’ina N. A. Novye sorta zhimolosti siney selektsii GNU YuUNIIPOK (New varieties of blue honeysuckle selection of the State South-Ural Research Institute of Fruit and Vegetable and Potato Cultivation), Selektsiya, semenovodstvo i tekhnologiya plodovo-yagodnykh kul’tur i kartofelya: sb. nauch.tr. Chelyabinsk, 2009, Vol. XI, pp. 74–80.

2. Savchenko I. V. Agrarnaya nauka Evro-Severo-Vostoka, 2017, No. 1 (56), pp. 4–9. (In Russ.)

3. Kulikov I. M., Minakov I. A. Agrarnaya nauka Evro-Severo-Vostoka, 2017, No. 1 (56), pp. 9–15. (In Russ.)

4. Nesmelova N. P., Somova E. N., Markova M. G. Sovershenstvovanie etapov klonal’nogo razmnozheniya zhimolosti siney (Perfection the stages of clonal propagation of honeysuckle blue), Proceedings of the X International Scientific and Practical Conference, Mordov. gos. un – t im. N. P. Ogareva, 2014, pp. 219–225. (In Russ.)

5. Nesmelova N. P., Somova E. N., Markova M. G. Izuchenie nekotorykh priemov na etapakh klonal’nogo mikrorazmnozheniya zhimolosti siney (The study of some techniques at the clonal micropropagation stages of honeysuckle blue), Proceedings of the Natianal Scientific Conference, Orlov. gos. un. – t, 2014, pp. 298–302. (In Russ.)

6. Valeev R. A., Kondrat’eva N.P., Kondrat’ev R. G. Izv. Mezhdunar. akad. agrar. obrazovaniya, 2013, No. 16, Vol. 1, pp. 23–25. (In Russ.)

7. Kondrat’eva N.P., Krasnolutskaya M. G., Bol’shin R. G. Ispol’zovanie progressivnykh elektrotekhnologiy elektrooblucheniya meristemnykh rasteniy (The use of advanced electrotechnologies for the electrical irradiation of meristem plants), Proceedings of the IV International Scientific Conference, April 24–25, 2015, Kazan», pp. 52–56. (In Russ.)

8. Markova M. G., Nesmelova N. P., Somova E. N. Ispol’zovanie svetodiodnykh obluchatel’nykh ustanovok v klonal’nom mikrorazmnozhenii yagodnykh kustarnikov (The use of LED irradiation facilities in the clonal micropropagation of berry bushes), Proceedings of the National Scientific and Practical Conference, Izhev. Gos. s. – kh. akad., Izhevsk, 2014, pp. 141–145. (In Russ.)

9. Morgunov D. N., Vasil’ev S. I. Izv. Orenburg. gos. agrar. un. – ta, 2016, No. 6 (62), pp. 75–77. (In Russ.)

10. Solovykh N. V., Budagovskiy A. V., Yankovskaya M. B. Agrarnaya nauka Evro-Severo-Vostoka, 2014, No. 5 (42), pp. 16–21. (In Russ.)

11. Valikov V.A., Vysotskiy V. A. Plodovodstvo i yagodovodstvo Rossii, 2013, Ch. 1 (38), pp. 82– 87. (In Russ.)

12. Markova M. G., Nesmelova N. P., Potapova S. A. Vestn. Don. GAU, 2015, ch. 1. No. 2 (16), pp. 104– 111. (In Russ.)

13. Vysotskiy V. A., Valikov V. A. Plodovodstvo i yagodovodstvo Rossii, 2013, Ch. 1 (7), pp. 57–62. (In Russ.)

14. Nesmelova N. P., Somova E. N. Vestn. NGAU, 2015, No. 3, pp. 25–31. (In Russ.)

15. Nesmelova N. P., Markova M. G. Vliyanie sposoba polucheniya na tekhnologicheskie kharakteristiki matochnykh rasteniy zhimolosti siney (Influence of the obtaining method on technological characteristics of grafters of blue honeysuckle), Proceedings of the Scientific and Practical Conference, Perm. NIISKh, Perm», 2013, Vol. 2, pp. 218–225. (In Russ.)

16. Kalinin F. L., Kushnir G. P., Sarnatskaya V. V. Tekhnologiya mikroklonal’nogo razmnozheniya rasteniy (Technology of microclonal reproduction of plants). Kiev, Nauk. dumka, 1992, 232 p.

17. Tekhnologiya proizvodstva bezvirusnogo posadochnogo materiala plodovykh, yagodnykh kul’tur i vinograda (Technology of production the virus-free planting stock of fruit, berry crops and grapes), Soyuzpitomnik, Gos. proizv. ob”edinenie. po proizvodstvy posadochnogo materiala, Moscow, 1989, 169 p.

18. Tekhnologicheskiy protsess polucheniya bezvirusnogo posadochnogo materiala plodovykh i yagodnykh kul’tur (The technological process of obtaining a virus-free planting stock of fruit and berry crops) metod. ukazaniya, MSKh RF, RASKhN, VSTISP, Moscow, 2001, 109 p.

19. Valeev R. A., Kondrat’eva N. P. Rezul’taty opytov po vliyaniyu spektra izlucheniya svetodiodov na meristemnye rasteniya (Results of experiments on the effect of the emission spectrum of LEDs on meristem plants), Proceedings of the VIII International Scientific and Practical Conference, Moscow, GNU VIESKh, 2012, Ch. 2, pp. 212–218. (In Russ.)

20. Nesmelova N. P., Somova E. N. Vladimirskiy zemledelets, 2015, No. 1, pp. 35–37. (In Russ.)

21. Dospekhov B. A. Metodika polevogo opyta s osnovami statisticheskoy obrabotki rezul’tatov issledovaniy (Methodology of field experience with the basics of statistical processing of research results), Moscow, Al’yans, 2011, 352 p.