MORPHOLOGICAL AND IMMUNE HYSTOCHEMICAL RESEARCH ON PIGS’ ADENOID TISSUE associated with conjunctival TISSUE OF PIGS
Abstract
Pig’s conjunctiva has local immune system, which is conjunctiva associated lymphoid tissue. It is represented by lymphoid cells and lymphoid tubercules as well as high endothelium venules in subepithelial connective tissue of conjunctiva connected with conjunctiva epithelium. The research shows that conjunctiva associated lymphoid tissue is a part of mucosal associated lymphoid tissue and morphologically similar to gut-associated lymphoid tissue and bronchoassociated lymphoid tissue. Immunohistochemical research methods are essential part of morphological research on lymphoid tissue of different animals. There are no data in literature about immunohistochemical research of pigs conjunctiva associated lymphoid tissue that are aimed at revealing main immune cells and their distribution. Immunohistochemical staining of paraffin section of pigs’ lids fixed in formaline by means of standard immunohistochemical methods (ABC and PAP) with application of primary antibodies to markers CD3, CD79а и PCNA showed positive reactions with Т-lymphocytes, В-lymphocytes and blast cells in conjunctiva associated lymphoid tissue. The paper describes location of Т-lymphocytes, В-lymphocytes and blast cells in conjunctiva associated lymphoid tissue. The authors found out morphofunctional changes in pigs’ conjunctiva associated lymphoid tissue in dependence of their age.
About the Authors
З. Каландарова
Кыргызский национальный аграрный университет им. К. И. Скрябина
Russian Federation
Russian Federation
М. Райнахер
Институт ветеринарной патологии
Russian Federation
Russian Federation
References
1. Knop E., Knop N. MALT tissue of the conjunctiva and nasolacrimal system in the rabbit and human// Vision Res. -1996. - Vol. 36. - P. 60.
2. Knop E., Knop N. Fine structure of high endothelial venules in the human conjunctiva// Ophthalmic Res. -1998. - Vol. 30. - P. 169.
3. Knop E., Knop N. High endothelial venules are a normal component of lymphoid tissue in the human conjunctiv and lacrimal sac// Invest. Ophthalmol. Vis. Sci. -1998. - Vol. 39. - P. 548.
4. Knop E., Knop N. Conjunctiva-associated lymphoid tissue (CALT) in the human eye - Components and topographical distribution// Ophthalmic Res. -1999. - Vol. 31. - P. 156.
5. Knop E., Knop N. Conjunctiva-associated lymphoid tissue (CALT) in the human eye - Morphometric analysis of lymphoid follicles// Ophthalmic Res. -1999. - Vol. 31. - P. 63.
6. Knop E., Knop N. Eye-associated lymphoid tissue (EALT) is continuously spread throughout the ocular surface from the lacrimal gland to the lacrimal drainage system// Ophthalmologe. -2003. - Vol.100. - P. 929-942.
7. Knop N., Knop E. Conjunctiva-associated lymphoid tissue in the human eye// Invest. Ophthalmol. Vis. Sci. -2000. - Vol. 42. - P.1270-1279.
8. Knop E., Knop N. The role of eye-associated lymphoid tissue in corneal immune protection // J. Anat. -2005. - Vol. 206. - P.271-285.
9. Franklin R.M, Remus L. E. Conjunctival-associated lymphoid tissue: evidence for a role in the secretory immune system// Inv. Ophthalmol. Vis. Sci. - 1984. - Vol. 25. - P.181-187.
10. Knop N., Knop E. Ultrastructural anatomy of CALT follicles in the rabbit reveals characteristics of M-cells, germinal centres and high endothelial venules// J. Anat. - 2005. - Vol. 207. - P. 409-426.
11. Fix A. S., Arp L. H. Conjunctiva-associated lymphoid tissue in normal and Bordetella avium-infected turkeys// Vet. Pathol. -1989. - Vol. 26. - P. 222-230.
12. Ruskell G. L. Organization and cytology of lymphoid tissue in the cynomolgus monkey conjunctiva// Anat. Rec. -1995. - Vol. 243. - P.153-164.
13. Ruskell G. L. Sensory innervation of conjunctival lymph follicles in cynomolgus monkeys// Vision Research. - Vol. 35. - P. 149.
14. Light microscopic studies on the conjunctiva associated lymphoid tissue (CALT) of Angora goats / R. N. Asti, N. Kurtdede, Н. Altunay, А. Ozen // AU Vet. Fak. Derg. - 2000. - Vol. 47. - P. 31-37.
15. Histological study of conjunctiva-associated lymphoid tissue in mouse / T. Sakimoto, J. Shoji, N. Inada [et al.] // Jap. J. Ophthalmol. -2002. - Vol.46. - P. 364-369.
16. Giuliano E. A., Moore C. P., Phillips T. E. Morphological evidence of M cells in healthy canine conjunctiva-associated lymphoid tissue// Graefes Arch. Clin. Exp. Ophthalmol. - 2002. - Vol. 240. - P. 220-226.
17. Astley R. A., Kennedy R. C., Chodosh J. Structural and cellular architecture of conjunctival lymphoid follicles in the baboon (Papio anubis) // Exp. Eye Res. -2003. - Vol. 76. - P. 685-694.
18. Ocular defense mechanisms with special reference to the demonstration and functional morphology of the conjunctiva-associated lymphoid tissue in Japanese monkeys / M. Kageyama, K. Nakatsuka, T. Yamaguchi [et al.] // Arch Histol Cytol. -2006. - Vol. 69 (5). - P.311-322.
19. Bayraktaroglu A. G., Asti R. N. Light and electron microscopic studies on Conjunctiva Associated Lymphoid Tissue (CALT) in cattle// Revue Med. Vet. - 2009. - Vol. 160, N 5. - P. 252-257.
20. Conjunctival Lymphoid Follicles in New World Rodents / R. A. Astley, J. Chodosh, W. Caire, G. M. Wilson // The Anatomical Record. -2007. - Vol. 290. - P.1190-1194.
21. Giuliano EA, Finn K. Characterization of membranous (M) cells in normal feline conjunctiva-associated lymphoid tissue (CALT) // Vet. Ophthalmol. - 2011. - Vol. 14, N 1. - P.60-66.
22. Каландарова З. К., Иргашев А. Ш., Арбаев К. С. Гистологическое строение конъюнктиво-ассоциированной лимфоидной ткани у свиней в возрастном аспекте // Вестн. АПК Верхневолжья. - 2016. - № 1 (33). - С. 55-59.
23. Liebler-Tenorio E., Pabst R. MALT structure and function in farm animals// Vet. Res. - 2006. - Vol. 37. - P. 257-280.
24. Асанова Э., Иргашев И. Ш., Manfred Rеinacher. Иммуногистохимическое исследование лимфатических узлов яка // Исследования, результаты. -2008. - № 1. - С. 182-185.
25. Иргашев А. Ш., Касиева Г. К. Результаты гистологических и иммуногистохимических исследований лимфатических узлов крупного рогатого скота// Наука, новые технологии и инновации. -2013. -№ 4. - С. 158-161.
26. Алдаяров Н. С., Иргашев А. Ш. Гистологические и иммуногистохимические методы при диагностике чумы собак// Аграр. вестн. Верхневолжья. -2016. -№ 3. - С. 58-64.
Review
For citations:
, MORPHOLOGICAL AND IMMUNE HYSTOCHEMICAL RESEARCH ON PIGS’ ADENOID TISSUE associated with conjunctival TISSUE OF PIGS. Bulletin of NSAU (Novosibirsk State Agrarian University). 2017;(2):86-93. (In Russ.)
Views:
203
Email this article 
