DEVELOPMENT OF IMMUNOTECHNIQUE USED FOR DIAGNOSTICS OF DOG ECHINOCOCCUS DISEASE

Echinococcus has become high endemic zoonotic disease in the former Soviet republics of Central Asia, i.e. Kazakhstan, due to higher slaughter and dehelminthization of dogs. Due to this fact, early recognition of dogs infected by Еchinococcus granulosus and environmental monitoring on parasites are very relevant and important. Certain methods of echinococcus diagnostics are inefficient. The paper reveals the results on development of immune and enzyme test for recognition of the dogs infected by echinococcus on the basis of finding the gid tapeworm antigene in excrements. The main agents of test system are rabbit polyclonal antibodies and mice monoclonal antibodies that are specific to excretory and secretory antigene of larvae and adult forms of Е. granulosus. The authors did experiment on the excrements of experimentally infected dogs and diagnostic agent has shown that it differentiates coproantigenes of two closely related gid tapeworms: Е. granulosus and T. hydatigena. Coproantigene of echinococcus was observed in the dogs excrements on the 5th days after being infected and was detected till the 30th day of the experiment. The research results speak about the possibility to use immune and enzyme analysis for diagnostics of echinococcus. The authors suggest to introduce immune and enzyme test into echinococcus diagnostics and set the coproantigene elimination after dehelminthization that can define the period of re-examination in order to confirm no parasites in the organism.

dog echinococcus, Echinococcus granulosus, diagnostics, immune and enzyme analysis, excretory and secretory antigene, polyclonal antibody, monoclonal antibody, эхинококкоз собак, Echinococcus granulosus, диагностика, иммуноферментный анализ, экскреторно-секреторный антиген, поликлональные антитела, моноклональные антитела

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