SCREENING STUDYING THE CATTLE REDNOSE IN VIRAL GENOME BACILLI

The research is aimed at screening studying of the cattle rednose in viral genome bacilli and was carried out in several stages. The first stage includes screening of virus antigen relation with bacteria in the process of agglutination with polyvalent serum. The second stage confirms general antigens in the process of indirect hemagglutination suppression. The third stage estimates anti-body producing after animals’ immunization with bacilli suspension in comparison with culture virus of the cattle rednose. The fourth stage defines the viral genome by means of real-time polymerase chain reaction with primers, homologous conserved region of glycoprotein D and glycoprotein B. The paper investigates 70 strains and concludes that 28.6% responded at 6,3–7,3 log₂ titer; 15.7% responded at 8,3 log₂ , 4,3% – 9,3 log₂ , 4,3–11,3 log₂ titer. The authors declare, 15 bacilli strains have antibodies entrapped to the cattle rednose virus on bacterial surface when studying in the process of indirect hemagglutination suppression; 10 strains contributed reducing of antibodies titer in 32–64 times; 4 strains reduced antibodies titer in 16 times; and 1 strain reduced it in 8 times. Polymerase chain reaction considers 3 strains to have homologous conserved region of glycoprotein D and glycoprotein B of the cattle rednose. This certifies the viral genome provides persistence in bacteria and they can produce specific antigens contributing to producing animals’ antiviral antibodies.

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