REACTIVE CHEMICALS IN SEROLOGIC DIAGNOSIS OF TOXOPLASMOSIS

The article reveals the results on studying immunological potency, immunogenicity and specific features of toxoplasmic antigen received from Тoxoplasma gondii RH culture and its applying in immune-enzyme analysis in order to make serological diagnosis. The authors applied toxoplasma produced from serebrum suspension and passed through the mice organisms in order to receive antigen. The research trained the method which allows accumulating parasitic mass with low concentration of exudative cells. The paper defines survival of toxoplasma and protection its virulent properties in keeping. The authors certify that used parasitic strain keeps biological activity and virulence during 17 days at 4оС. Toxoplasmic antigen was of high immunological potency and allowed receiving hyperimmune sheep serum at 1 : 51200 titer in immune-enzyme analysis. The specimen 1 : 10 solution responses positively with antiserum at 1 : 5 titer and 1 : 10 in immune-enzyme analysis; it didn’t affect antibodies subject to chlamidia, besnoitia and brucella. Studying protein concentration of toxoplasmic antigen by means of electrophoresis showed major protein with molecular mass 140 kilodalton. The research demonstrates that reactive chemical Active T.gondi p30 protein fragment (Abcam) applied as parasitic protein with molecular mass 30 kilodaltonresponded equally to hyperimmune toxoplasmic serum; this certifies antigen similarity of the specimens compared. The authors conclude there is a possibility to apply toxoplasmic antigen and hyperimmune antiserum in development of immune-enzyme diagnosis.

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