THE INFLUENCE OF EXPERIMENTALALIMENTARY OBESITY ON THE STRUCTURE OF VISTAR LINE RATS’LIVER

At the present time obesity is an important social and medical problem. The liver is a unique organ where all metabolic ways cross and principle exchange processes run. Therefore the aim of research was to reveal and estimate the character of structural changes in the liver of Vistar line rats in the model of alimentary obesity. The objectives of the research are to carry out morphometric and light optics examination of the liver, to analyze the morphometric data in the structure and cell composition of the organ on the light optics level at experimental obesity of alimentary etiology. The experiment involved 2-month old sexually mature femalerats of Vistar line with the initial body weight 180–200 g. There were two groups of animals in the experiment: control (intact rats receiving standard laboratory food diet) and experimental (receiving the model of standard laboratory food diet added to by edible fats of animal origin during 3 months). The animals were slaughtered under etaminal anesthesia (40 mg per 1 kg of animal body weight) by decapitation. For the morphometric and light optics examinations (microscope LEICA DM 750, camera LEICA ICC 50 HD) histologic preparations were fixed in 10% buffered formalin. The morphometric examination of liver preparations was carried out with 1000-folded magnification in the cuts of 5mcm thick and stained with Mayer's hematoxylin and eosin using the method of applying point morphometric grids (a grid of 256 points). Relative areas of sinusoid networks, nuclei, and cytoplasm of hepatocytes, numerical densities of sinusoid cells, hepatocytes and binucleated parenchyma cells were determined; nuclear-cytoplasm ratio, the ratio of numerical density of sinusoid cells to the numerical density of all hepatocytes were calculated; the part of diplokaryocytes was calculated from the total number of hepatocytes, Vizotto coefficient was used to calculate the ratio of sinusoid network area to the area of parenchyma of all the hepatocytes. The experimental alimentary obesity is established to result in adipose dystrophy in liver parenchyma and simultaneous stimulation of hepatocytes functional activity. Structural changes in the parenchyma cells are concomitant with functional tension of capillary-connective tissue structures expressed by disorders in blood circulation and lymph flow in the organ.

liver, alimentary obesity, Vistar line rats

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