MORPHOFUNCTIONAL CHANGES OF SPLEEN OF BALB/C MICE SUFFERED FROM RAUSCHER LEUKEMIA VIRUS

Abstract

The research shows that application of Rauscher leucosis virus leads to 21.9 times increasing of lien; disease progress results in wearing borders of white and red pulp and various bloodstrokes and necrosis areas appear. The authors observed many erythrocytes in red pulp lien atriums in 2 month of research in hyperplastic period. This results in reducing of atriums square; splenic lymph follicles grow and interlock each other; many irregular blast cells appear in all parts of lien diffusely. Progress of viral leucosis comes with immune system suppression of responding mice; immune suppression is observed in B-cell chain of immune system and T-cell chain. In 2 months after infecting mice with Rauscher virus, the authors observed less spontaneous proliferation in splenocytes compared with the control group (experiment/control = 1067/8072) and reducing of ConA and PWM stimulated cell proliferation (experiment/control = 1934/30603) and (experiment/control = 1178/2033) correspondently. Progress of viral leucosis comes with low spontaneous and ConA stimulated proliferation of splenocytes and high PWM stimulated proliferation in comparison with the control group. Index of spontaneous, ConA and PWM stimulated proliferation increased from the 2nd to 11th month of the research. The authors observed increasing of the number of medium lymph in cell composition of lien white pulp; the number of plasmoblast, erythroblast, plasmocytes and megakaryocytes is increasing in the red pulp. The number of erythroblast in the red pulp increased in the end-stage.