Amino acid composition of biosubstances depending on the method of maral velvet antler extraction
https://doi.org/10.31677/2072-6724-2025-75-2-200-206
Abstract
The aim of this study is to determine the most effective method for extracting maral velvet antlers based on the obtained data on amino acid composition. The research involved the production of biosubstances using 4 extraction methods: 1st method: Ultrasound extraction (22 kHz) with a raw material-to-water ratio of 1:10 at a temperature of 45–50 °C; 2nd method: Ultrasound extraction (22 kHz) with a raw material-to-water ratio of 1:10 at 45–50 °C, followed by autoclaving; 3rd method: Ultrasound extraction (37 kHz) with proteolytic enzymes, with a raw material-to-water ratio of 1:10 at 45–50 °C; 4th method: Ultrasound extraction (37 kHz) with microbial enzymes, with a raw material-to-water ratio of 1:10 at 45–50 °C, followed by autoclaving. Amino acid analysis was performed using chromatography on a Shimadzu LC-20 Prominence system equipped with a diode array detector. The calibration curve was constructed using nineteen standard amino acid samples manufactured by Sigma (Germany). The study results indicate that the second and fourth extraction methods are the most effective. These methods yield biosubstances with amino acid concentrations 1.4–2.1 times higher than those obtained using other extraction techniques.
About the Authors
M. G. Krotova
Federal Altai Scientific Center of Agrobiotechnology (All-Russian Research Institute of Antler Reindeer Breeding)
Russian Federation
Russian Federation
Candidate of Agricultural Sciences
Barnaul
I. N. Grishaeva
Federal Altai Scientific Center of Agrobiotechnology (All-Russian Research Institute of Antler Reindeer Breeding)
Russian Federation
Russian Federation
Candidate of Biological Sciences
Barnaul
А. I. Korolkova
Federal Altai Scientific Center of Agrobiotechnology (All-Russian Research Institute of Antler Reindeer Breeding)
Russian Federation
Russian Federation
Candidate of Agricultural Sciences
Barnaul
A. A. Nepriyatel
Federal Altai Scientific Center of Agrobiotechnology (All-Russian Research Institute of Antler Reindeer Breeding)
Russian Federation
Russian Federation
Doctor of Agricultural Sciences
Barnaul
I. S. Belozerskyh
Federal Altai Scientific Center of Agrobiotechnology (All-Russian Research Institute of Antler Reindeer Breeding)
Russian Federation
Russian Federation
Research Fellow
Barnaul
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Review
For citations:
Krotova M.G., Grishaeva I.N., Korolkova А.I., Nepriyatel A.A., Belozerskyh I.S. Amino acid composition of biosubstances depending on the method of maral velvet antler extraction. Bulletin of NSAU (Novosibirsk State Agrarian University). 2025;(2):200-206. (In Russ.) https://doi.org/10.31677/2072-6724-2025-75-2-200-206
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