Polyfunctional effect of Bacillus licheniformis bacterial strains on a fruiting plantation of garden strawberry
https://doi.org/10.31677/2072-6724-2025-75-2-36-45
Abstract
In a production experiment in production strawberry plantings in the agricultural cooperative “Gardens of Siberia” (Novosibirsk region) in 2022–2024, the most effective stimulation of the adaptive properties and vegetative propagation of plants under the influence of pre-planting treatment of the root system of seedlings with bacterial strains Bacillus licheniformis VKPM B-10561, B. licheniformis VKPM B-10562, B. licheniformis VKPM B-10563 and B. licheniformis VKPM B-10564 at concentrations of 1×105 CFU/ml was proven. The survival rate of planted plants increased by 13–17 % relative to the control. The number of daughter rosettes in productive plants increased by 33–58 %, to a level of 4.8–5.7 rosettes/plant. The strains B. licheniformis VKPM B-10563 and B. licheniformis VKPM B-10564 had the greatest growth-stimulating and protective effect against fungal infectious diseases. The number of leaves per plant increased by 14–21 %, the biomass of one plant – by 42–48 %, the length of roots – by 10–16 %, relative to the control. Against white leaf spot, fusarium and verticillium wilt, these strains acted with biological efficiency from 41 to 81 %. Stimulation of strawberry yield in all variants with pre-planting treatment with bacterial strains by 0.3–1.0 t/ha was proven. Under the influence of the B. licheniformis strain VKPM B-10561, the yield increased by 35 %, with 2.7 t/ha in the control variant, B. licheniformis VKPM B-10562 – by 16 %, B. licheniformis VKPM B-10563 – by 10 % and B. licheniformis VKPM B-10564 – by 30 %.
About the Authors
R. R. KolokolovRussian Federation
Postgraduate Student
Novosibirsk
A. A. Belyaev
Russian Federation
Doctor of Agricultural Sciences, Associate Professor
Novosibirsk
References
1. Gosudarstvennyj katalog pesticidov i agrohimikatov, razreshennyh k primeneniju na territorii Rossijskoj Federacii. Part I. Pesticidy (State catalog of pesticides and agrochemicals, approved for use on the territory of the Russian Federation. Part I. Pesticides), Ministerstvo sel’skogo hozjajstva RF, Moscow, 2023, 945 p. (In Russ.)
2. Shternshis M.V., Belyaev A.A., Cvetkova V.P., Shpatova T.V., Leljak A.A., Bahvalov S.A., Biopreparaty na osnove bakterij roda Bacillus dlja upravlenija zdorov’em rastenij (Bacillus-based biological products for plant health management), Novosibirsk: Izdatel’stvo SO RAN, 2016, 233 p.
3. Miljakovic D., Marinkovic J., Balesevic-Tubic S., The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops, Microorganisms, 2020, Vol. 8, pp. 1037.
4. Maslienko L.V., Voronkova A.H., Dacenko L.A., Efimceva E.A., Punogina D.A., Gajdukova S.A., Kazakova V.V., Kovaleva S.R., Maslichnye kul’tury, 2020, Vol.. 3, No. 183, pp. 107–113. (In Russ.)
5. Sidorova T.M., Asturova A.M., Allahverdjan V.V., Jug Rossii: jekologija, razvitie, 2021, Vol. 16, No. 4, pp. 86–103. (In Russ.)
6. Lastochkina O.V., Sel’skohozjajstvennaja biologija, 2021, Vol. 56, No. 5, pp. 843–867. (In Russ.)
7. Jakuba G.V., Maslienko L.V., Gusin D.N., Plodovodstvo i vinogradarstvo Juga Rossii, 2013, Vol. 22, No. 4, pp. 81– 88. (In Russ.)
8. Beljaev A.A., Leljak A.I., Leljak A.A., Nevolin S.V., Shpatova T.V., Shahristova A.A., Judushkin V.V., Dostizhenija nauki i tehniki APK, 2017, No. 5, pp. 20–23. (In Russ.)
9. Stol’nikova N.P., Lutov V.I., Promyshlennaya kul’tura zemlyaniki v Sibiri (Industrial culture of strawberries in Siberia), NGAU. NIISS im. M.A. Lisavenko, Novosibirsk, 2009, 207 p.
10. Metodika vyjavlenija i ucheta boleznej plodovyh i jagodnyh kul’tur (Methodology for detecting and recording diseases of fruit and berry crops), Moscow: Kolos, 1971, 23 p. (In Russ.)
11. Chumakov A.E., Zaharova T.I., Vredonosnost’ boleznej sel’skohozjajstvennyh kul’tur (Harmfulness of crop diseases), Moscow: Agropromizdat, 1990, 127 p.
12. Programma i metodika sortoizucheniya plodovykh, yagodnykh i orekhoplodnykh kul’tur (Program and methodology for variety study of fruit, berry and nut crops), Orel, 1999, 606 p.
13. Dospekhov B.A., Metodika polevogo opyta: s osnovami statisticheskoi obrabotki rezul’tatov issledovanii (Field experience methodology: with the basics of statistical processing of research results), Moscow, 2013, 349 p.
14. Sorokin O.D., Prikladnaya statistika na komp’yutere (Applied statistics on the computer), Krasnoobsk: GUP RPO SO RASKhN, 2009, 222 p.
15. Beljaev A.A., Kolokolov R.R., Kazakova O.A., Lutov V.I., Vestnik NGAU (Novosibirskij gosudarstvennyj agrarnyj universitet), 2023, No. 4, pp. 5–13. (In Russ.)
Review
For citations:
Kolokolov R.R., Belyaev A.A. Polyfunctional effect of Bacillus licheniformis bacterial strains on a fruiting plantation of garden strawberry. Bulletin of NSAU (Novosibirsk State Agrarian University). 2025;(2):36-45. (In Russ.) https://doi.org/10.31677/2072-6724-2025-75-2-36-45