Carbohydrate and mineral metabolism in romanov sheep in Western Siberia

The article presents information regarding the metabolism of carbohydrates and minerals in Romanov sheep in the Kemerovo region. The correlations among macro elements and carbohydrate metabolism indices and heavy metal levels in organs and tissues were ascertained. We determined the average content, variability and reference intervals of glucose, amylase, lactate dehydrogenase and macro elements in blood serum. We investigated the relationship between these indices and heavy metal levels in sheep organs and tissues. In the Romanov sheep breeding zone, heavy metal content was analyzed in soil, water, and feed and did not exceed the MAC. The determination of the content of macroand microelements was carried out in the laboratory of isotopegeochemical methods of analysis at the V.S. Sobolev Institute of Geology and Mineralogy of the Siberian Branch of the Russian Academy of Sciences by atomic emission spectrometry employing the NSAM analysis technique No. 450-S. The level of blood serum glucose is 6.0 mmol/l, the amylase is 104.0 U/l, and the lactate dehydrogenase is 1086 U/l. The order of macro elements in serum is as follows: Na > K > Ca > P > Mg in accordance with 90 : 7 : 4 : 3.4 : 1. The concentration of K in the blood exhibits a significant correlation with Zn in the spleen (r = 0.507) and Cu in the testes (r = 0.535). Simultaneously, Na exhibited a negative correlation with the concentration of Zn in the heart (r = -0.416) and Pb in the blood (r = −0.598). There was a high negative relationship between Ca in the blood and the frequency of chromosomal breaks (r = −0.752). The glucose level was negatively correlated with the concentration of Cu in the muscles (r = −0.414), Zn in the kidneys (r = −0.465), and Zn in the heart (r = −0.572), but its level was positively associated with Pb in the muscles (r = 0.568). The significance of the indicators of carbohydrate and mineral metabolism, as well as the correlations between the studied parameters, is evident in the comprehensive evaluation of the breeds phenopool.

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