Application of Bacillus bacterial strains in the propagation of honeysuckle by woody cuttings

The studies were conducted in three-year production experiments in the nursery of the agricultural production cooperative (APC) “Sady Baraby” in the Novosibirsk region to assess the effectiveness of the Bacillus bacteria strains in obtaining honeysuckle seedlings from lignified cuttings. The most effective stimulation of rooting of lignified honeysuckle cuttings was proven with pre-planting treatment with the strains B. amyloliquefaciens VKPM B-10642, B. licheniformis VKPM B-10562 and a mixture of 3 strains (experimental biopreparation Fitop 8.67) in concentrations of 1×10⁵ CFU/ml to an absolute level of 36.9–40.5 %, 20.3–32.1 % higher than the control. All the studied bacterial strains reliably stimulated the increase in seedling biomass by 17.6–42.6% relative to the control, to a level of 11.7–14.2 g/plant. The most stable in the years of observation were the strain B. amyloliquefaciens VKPM B-10642 and a mixture of three strains, which can be stated to have the greatest growthstimulating effect on honeysuckle plants. An increase in the commercial quality of seedlings (I and II grades) was proven when using pre-planting treatment with the strains B. subtilis VKPM B-10641, B. licheniformis VKPM B-10562 and a mixture of three strains by 32.5–35.0 % relative to the control, to an absolute level of 70.7–72.0%. The highest yield of standard seedlings, on average over 3 years, was demonstrated with pre-planting treatment with the B. licheniformis strain VKPM B-10562 – 777.4 seedlings/variant, which should be recognized as the most promising strain for use in obtaining seedlings from lignified honeysuckle cuttings.

1. Gosudarstvennyj katalog pesticidov i agrohimikatov, razreshennyh k primeneniju na territorii Rossijskoj Federacii. Part I. Pesticidy (State catalog of pesticides and agrochemicals approved for use on the territory of the Russian Federation. Part I. Pesticides), Ministerstvo sel’skogo hozjajstva RF, Moscow, 2023, 945 p. (In Russ.)

2. Petrusha E.N., Krykova A.S., Dal’nevostochnyj agrarnyj vestnik, 2018, No. 2 (46), pp. 39–43. (In Russ.)

3. Shternshis M.V., Belyaev A.A., Cvetkova V.P., Shpatova T.V., Leljak A.A., Bahvalov S.A., Biopreparaty na osnove bakterij roda Bacillus dlja upravlenija zdorov’em rastenij (Bacillus-based biological products for plant health management), Novosibirsk: Izdatel’stvo SO RAN, 2016, 233 p.

4. Rjabchinskaja T.A., Zimina T.V., Agrohimija, 2017, No. 12, pp. 62–92. (In Russ.)

5. Calvo P., Nelson L., Kloepper J.W., Agricultural uses of plant biostimulants, Plant Soil., 2014, Vol. 383(1), pp. 3–41.

6. Kim P.I., Production of biosurfactant lipopeptides iturin A, fengycin an.d surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides, J. Microbial Biotechnology, 2010, Vol. 20, pp. 138–145.

7. Pavljushin V.A., Novikova I.I., Bojkova I.V., Sel’skohozjajstvennaja biologija, 2020, Vol. 55, No. 3, pp. 421–438. (In Russ.)

8. Perez-Garsia A., Romero D., Vicentedei A., Plant protection and growth stimulation by microorganisms, Curr Opinion Biotechnol., 2011, Vol. 22(2), pp. 187–193.

9. Saraf M., Pandya U., Thakkar A., Role of allelochemicals in plant growth promoting rhizobacteria for biocontrol of phytopathogens, Microb. Res., 2014, Vol. 169, pp. 18–29.

10. Beljaev A.A., Shternshis M.V., Shpatova T.V., Lutov V.I., Leljak A.A., Leljak A.I., Judushkin V.V., Dostizhenija nauki i tehniki APK, 2012, No. 3, pp. 16–19. (In Russ.)

11. Beljaev A.A., Shternshis M.V., Shpatova T.V., Lutov V.I., Leljak A.A., Leljak A.I., Judushkin V.V., Dostizhenija nauki i tehniki APK, 2014, No. 1, pp. 38–40. (In Russ.)

12. Proizvodstvo i sertifikacija posadochnogo materiala plodovyh, godnyh kul’tur i vinograda v Rossii. Kontrol’ kachestva. Part 1. Jagodnye kul’tury (Production and certification of planting material for fruits, suitable crops and grapes in Russia. Quality control. Part 1. Berry crops), Moscow: VSTISP, 2009, 164 p.

13. Programma i metodika sortoizucheniya plodovykh, yagodnykh i orekhoplodnykh kul’tur (Program and methodology for variety study of fruit, berry and nut crops), Orel: Izd-vo VNIISPK, 1999, 606 p.

14. Dospekhov B.A., Metodika polevogo opyta (s osnovami statisticheskoi obrabotki rezul’tatov issledovanii) (Field experience methodology (with the basics of statistical processing of research results), Moscow, 2013, 349 p.

15. Sorokin O.D., Prikladnaya statistika na komp’yutere (Applied statistics on the computer), Krasnoobsk: GUP RPO SO RASKhN, 2009, 222 p.