Distribution of genotypes by the growth diferential factor 9 (GDF-9) gene locus in the romanov breed sheep population
https://doi.org/10.31677/2072-6724-2024-72-3-196-204
Abstract
The FecG gene, also known as GDF-9 (growth factor differentiation factor 9), plays an important role in regulating reproductive function in mammals. This paper presents the results of a study of a point mutation in the differential growth factor gene in Romanov breed sheep. Mutations in exon 1 of the GDF-9 gene can lead to disturbances in ovarian development and ovulation, resulting in infertility or reduced reproductive capacity in animals. The object of the study was Romanov breed sheep in a sample of 30 individuals. For DNA extraction, venous blood samples were taken from the jugular vein. In Romanov breed sheep for the GDF-9 gene, it was found that at the G1 locus of this gene there are two alleles – G and A. Three genotypes were also identified: GG (254 bp/117 bp), AG (410bp/254/117 bp) and AA (410 bp/410 bp). The observed χ2 criterion was less than expected, therefore, the empirical and theoretical frequencies do not differ significantly, and deviations from the distribution according to the Hardy-Weinberg law are not observed. The observed heterozygosity index (Ho) was 0.27. Allele frequencies were 0,8 for G and 0,2 for A. For genotypes the following were obtained: GG – 0,66, GA – 0,27 and AA – 0,07.It can be noted that the data on the frequencies of alleles and genotypes in the population of Romanov breed sheep differ from the results for the Kulunda fine-wool, Dagestan mountain, Salsk, Lacaune and Merino breeds. The research results can be used to monitor changes in allele frequencies and genotypes in sheep during the selection process.
About the Authors
E. A. Klimanova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
E.A. Klimanova, researcher
Novosibirsk
T. V. Konovalova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
T.V. Konovalova, senior lecturer
Novosibirsk
O. S. Korotkevich
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
O.S. Korotkevich, Doctor of Biological Sciences, Professor
Novosibirsk
V. L. Petukhov
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
V.L. Petukhov, Doctor of Biological Sciences, Professor
Novosibirsk
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Review
For citations:
Klimanova E.A., Konovalova T.V., Korotkevich O.S., Petukhov V.L. Distribution of genotypes by the growth diferential factor 9 (GDF-9) gene locus in the romanov breed sheep population. Bulletin of NSAU (Novosibirsk State Agrarian University). 2024;(3):196-204. (In Russ.) https://doi.org/10.31677/2072-6724-2024-72-3-196-204
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