Influence of the complex of chitosan-ß-cyclodextrin with levofloxacin on the wounds’ microflora and the Sturgeon’s colon intestine

The authors in the article presented the results of determining the effectiveness of using the chitosan-ß-cyclodextrin complex with levofloxacin in the healing of mechanical wounds of valuable commercial fish sturgeons and their feeding. The experiment was conducted based on the “Progressive biotechnologies in aquaculture” research laboratory of the Saratov State University of Genetics, Biotechnology and Engineering. N.I. Vavilov. The microflora of incised wounds and the large intestine of sturgeon fingerlings under the influence of fluoroquinolone, represented by levofloxacin based on cyclodextrin, included in the shell of high-molecular chitosan, was studied. The studied microbiological indicators were chosen to determine that changes in the total number of microorganisms show the nature of the course of the inflammatory/pathological process, which contributes to the development of microorganisms (including opportunistic pathogens) and lactic acid bacteria in the intestine. Therefore, they are essential physiological indicators of the formation of “ intestinal immunity. It was found that the use of cyclodextrin with levofloxacin in the treatment of incised wounds in sturgeons leads to a significant decrease in the total microbial number (TMC) on their surface (by 10 thousand times compared to the group without treatment). It has been shown that using cyclodextrin with levofloxacin in feeding sturgeons reduces the total microbial number in the large intestine. This complex is characterised by good solubility and bioavailability for fish. The future study results can be used in aquaculture to treat mechanical injuries received during transportation and sorting in the rearing process in fish.

1. Gulyuk N.G., Puchkova T.S., Pihalo D.M., Komarov YU.I., Dostizheniya nauki i tekhniki APK, 2014, T. 28, No. 10, рр. 72–73. (In Russ.)

2. Deigen I.M., Egorov A.M., Kudryashova E.V., Vestnik Moskovskogo universiteta. Seriya 2. Khimiya, 2015, T. 56, No. 6, рр. 387–392. (In Russ.)

3. Kedik S.A., Panov A.V., Tyukova V.S., Zolotareva M.S., Razrabotka i registratsiya lekarstvennykh sredstv, 2016, No. 3, рр. 68–75. (In Russ.)

4. Dushkin A.V., Suntsova L.P., Khalikov S.S., Pharmaceutical sciences, 2013, No. 1, рр. 448–457. (In Russ.)

5. Drannikov A.A. i dr., Razrabotka i registratsiya lekarstvennykh sredstv, 2022, T. 11, No. 2, рр. 102–108. (In Russ.)

6. Serkova A.N. i dr., Fundamental’nye issledovaniya, 2015, No. 2–3, рр. 518–522, URL: https://fundamental-research.ru/ru/article/view?id=36845 (data obrashcheniya: 10.11.2022). (In Russ.)

7. Jernberg C., Löfmark S., Edlund C., Jansson J.K. Long-term ecological impacts of antibiotic administration on the human intestinal microbiota, The ISME journal, 2007, Vol. 1, No. 1, рр. 56– 66.

8. Alekseev A.A., Krutikov M.G., Yakovlev V.P., Rossiiskii meditsinskii zhurnal, 1997, No. 5, рр. 24–30. (In Russ.)

9. Privol’nev V.V., Karakulina E.V., Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya, 2011, T. 13, No. 3, рр. 214–222. (In Russ.)

10. Shul’ga E.A., Grozesku Yu.N., Bakhareva A.A., Vestnik AGTU. Seriya: Rybnoe khozyaistvo, 2009, No. 1, рр. 86–89. (In Russ.)

11. Sovremennye problemy i perspektivy razvitiya rybokhozyaistvennogo kompleksa, Sbornik trudov IX nauchno-prakticheskoi konferentsii, 2021, рр. 194–196. (In Russ.)

12. Danilevskaya N.V., Rossiiskii veterinarnyi zhurnal. Melkie domashnie i dikie zhivotnye, 2008, No. 1, рр. 28–31. (In Russ.)

13. Labinskaya A.S., Mikrobiologiya s tekhnikoi mikrobiologicheskikh issledovanii (Microbiology with the technique of microbiological research), Moscow: Meditsina, 1978, 394 р. (In Russ.)

14. Vorob’ev V.YA., Elsukov A.I., Teoriya i eksperiment (Theory and experiment), Minsk: Vysshaya shkola, 1989, 109 p. (In Russ.)

15. Kulikov S.N., Khairullin R.Z., Tyurin Yu.A., Kazanskii meditsinskii zhurnal, 2010, No. 5, рр. 656–660. (In Russ.)

16. Topuriya L.Yu., Topuriya G.M., Veterinarnoe delo, 2010, No.1, рр. 61–68. (In Russ.)