Effect of metformin on the development of Sarcoma Walker-256
https://doi.org/10.31677/2072-6724-2021-60-3-107-114
Abstract
There has been a great deal of interest in veterinary medicine in drugs that support the body during chemotherapeutic treatment. The authors conducted a study of the drug’s effect on the development of carcinosarcoma in the mono regimen. The growth pattern of artificially induced Walker-256 sarcoma in 3-month-old Wistar rats in the post-lactation period was studied. The study was conducted at the Department of Pharmacology and General Pathology, Faculty of Veterinary Medicine.
The study was conducted at the Department of Pharmacology and General Pathology, Faculty of Veterinary Medicine. The objects of the study were Wistar rats, female rats of three months of age, weighing 150-200 g in the post-lactation period. Rats of both groups were infected with Walker 256 sarcoma by injection into the thigh muscle at a dose of 106 ml/head. Rats in the experimental group were additionally administered metformin at 30 mg/head daily with water. Histological, cytological and haematological studies were performed on days 14 and 21 from the time of infection of the rats with the tumour. Under the action of metformin, the number of erythrocytes, leukocytes, lymphocytes, monocytes and granulocytes in Walker-256 sarcoma-infected rats increased, the concentration of haemoglobin decreased, and the number of platelets did not change. In the biopsy specimen of control rats compared to the counterparts of the experimental groups, the concentration of monocytes was increased. The number of eosinophils and nuclear polymorphism was decreased under the influence of metformin. In addition, metformin-induced neuropsychiatric abnormalities in rats, such as appetite perversion and cannibalism. The more pronounced inflammatory response in the experimental group indicates that further study of the drug’s effect on the development of various neoplasms is necessary, as this is an essential factor in the choice of the treatment strategy.
About the Authors
G. A. Nozdrin
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Doctor of Veterinary Sciences, Professor
Novosibirsk
A. S. Gavrilovich
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Postgraduate student
Novosibirsk
References
1. Korman D.B., Misheni i mekhanizmy deystviya protivoopukholevykh preparatov (Targets and mechanisms of action of antitumor drugs), Moscow: Prakticheskaya meditsina, 2014, 149 p.
2. Prospective identification of tumorigenic breast cancer cells / M. Al-Hajj, M.S. Wicha, A. BenitoHernandez [et al.], Proc. Natl. Acad, Sci USA, 2003, – No. 7, pp. 100.
3. Kushlinskiy N.E., Nemtsova M.V., Chelovek i ego zdorov’e, 2014, No. 1-2, pp. 5–15 (In Russ.).
4. Dolzhikov A.A., Churnosov M.I., Bykov P.M. [i dr.], Chelovek i ego zdorov’e, 2010, No. 2, pp. 149–155 (In Russ.).
5. Vinogradova T.V., Chernov I.P., Monastyrskaya G.S., Acta naturae, 2015, No. 4, pp. 53–63.
6. Alfarouk K.O., Verduzco D., Rauch C., Glycolysis, tumor metabolism, cancer growth and dissemination. A new pH-based etiopathogenic perspective and therapeutic approach to an old cancer question, Oncoscience, 2014, No. 12, pp. 777-802.
7. Mingaleeva R.N., Miftakhova R.R., Rizvanov A.A., Geny i kletki, 2015, No. 2, pp. 11–14 (In Russ.).
8. Foretz M., Hebrard S., Leclerc J. [et al.], Metformin inhibits hepatic gluconeogenesis in mice independently of the LKB1/ AMPK pathway via a decrease in hepatic energy state, Ckin invest, 2010, Vol. 1, pp. 2355–2369.
9. Mushkambarov N.N., Kuznetsov S.S., Molekulyarnaya biologiya (Molecular Biology), Moscow: Med. inform. Agenvstvo, 2007, pp. 501–503.
10. Lee E., Pre-treatment of mice with tumor-conditioned media accelerates metastasis to lymph nodes and lungs: a new spontaneous breast cancer metastasis model, Clin Exp Metastasis, 2014, Vol. 31, pp. 67–79.
11. Kupriyanov V.V., Mironov V.A., Mironov A.A., Gurina O.Yu., Angiogenez: obrazovanie, rost i razvitie krovenosnykh sosudov (Angiogenesis: formation, growth and development of blood vessels), Moscow: NIO «Kvartet», 1993, pp. 152.
12. Liu B., Fan Z., Edgerton S.M., Metformin induces unique biological and molecular responses in triple negative breast cancer cells, Cell Cycle, 2009, Vol. 8, pp. 2031–2040
13. Boyakhchan A.A., Adil’khanov A.V., Akhuba L.G., Shek D.L., Vospalenie kak odin iz osnovnykh faktorov razvitiya zlokachestvennykh novoobrazovaniy v organizme cheloveka, European journal of biomedical and life sciences, 2016, Vol. 4, pp. 40–43.
14. Vrioni F.D., Wu J.K., Vrionis F.D., Qi P.,Tumor cells expressing the herpes simplex virus-thymidine kinase gene in the treatment of Walker 256 meningeal neoplasia in rats, Neurosurg, 1996, Vol. 2, pp. 250–257.
15. Mayborodin I.V., Krasil’nikov S.E., Kozyakov A.E. [i dr.], Novosti khirurgii, 2015, No. 3, pp. 339–347 (In Russ.).
16. Kryuchkov A.N., Freynd G.G., Arkhiv patologiy, 2008, T. 70, No. 1, pp. 62–65 (In Russ.).
17. Nozdrachev A.D. Anatomiya krysy (Anatomy of a rat), Sankt-Peterburg: Lan’, 2001, pp. 237–239.
18. Shevchenko Yu.L., Kitaev V.M., Khirurgiya: Zhurnal im. N.I. Pirogova, 2010, No. 9, pp. 4–8 (In Russ.).
Review
For citations:
Nozdrin G.A., Gavrilovich A.S. Effect of metformin on the development of Sarcoma Walker-256. Bulletin of NSAU (Novosibirsk State Agrarian University). 2021;(3):107-114. (In Russ.) https://doi.org/10.31677/2072-6724-2021-60-3-107-114
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