Association of β-lactoglobulin genotypes with some biochemical parameters of the blood of Romanov sheep
https://doi.org/10.31677/2072-6724-2020-57-4-82-87
Abstract
The assessment of the gene pool and phenofund of agricultural breeds is an issue in livestock breeding. The accuracy of molecular genetic methods is much higher than phenotypic observation in order to improve breeds. It is necessary to establish relationship between the genotype and the desired trait for their successful application. Therefore, the purpose of our study was to study the relationship of genotypes at the β-Lg locus with some biochemical blood parameters in Romanov sheep. The relationship of genotypes at the β-Lg locus with the amount of albumin, urea and uric acid in Romanov sheep was studied. On a population of 23 sheep of the Romanov breed, we studied the polymorphism of the β-lactoglobulin gene. Blood sampling was carried out from sheep according to standard methods. The analysis was performed in the laboratory of ecological and veterinary genetics and biochemistry of the Novosibirsk State Agrarian University. Standard kits for biochemical studies were used for the analysis. The level of biochemical parameters was determined using a Photometer 5010 V5 + biochemical analyzer. DNA isolation was carried out according to the standard procedure. To establish genotypes, the PCR-RFLP method was used. It was found that in the Siberian population of Romanov sheep the β-LGA allele is more common than β-LGB. In homozygous B / B animals, the albumin concentration was 1.2 times higher (P <0.05) than in individuals with the A / A genotype. No relationship was found between the β-Lg genotypes and the amount of urea and uric acid in the blood.
About the Authors
E. A. Klimanova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
PhD student
Novosibirsk
T. V. Konovalova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Senior Professor
Novosibirsk
V. A. Andreeva
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
PhD student
Novosibirsk
O. S. Korotkevich
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Doctor of Biological Sc., Professor
Novosibirsk
V. L. Petukhov
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Doctor of Biological Sc., Professo
Novosibirsk
Yu. S. Nazarenko
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
PhD student
Novosibirsk
References
1. Kushnir A.V., Glazko V.I., Petukhov V.A., Dimov G., Storozhuk S.I., Biologiya, genetika i selektsiya ovtsy (Biology, genetics and sheep breeding), Novosibirsk: NGAU, 2010, 524 p.
2. Wafaa I.I., Plant Archives, 2019, No.19, pp. 284–288.
3. Syso A.I., Sokolov V.A., Petukhov V.L., J. Pharm. Sci. and Res, 2017, No. 9 (4), pp. 368–374.
4. Skiba T.V., Tsygankova A.R., Borisova N. S., Journal of Pharmaceutical Sciences and Research, 2017, Vol. 9 (6), pp. 958–964.
5. Korotkevich O.S., Lyukhanov M.P., Petukhov V.L., Yudin N. S., Proceedings of the 10th World Congress on Genetics Applied to Livestock Production, Vancouver, Canada, August 17–22, Publishing office: Promega, 2014, pp. 487.
6. Konovalova T.V., Narozhnykh K.N., Petukhov V.L., Fedyaev Y.I., Journal of Trace Elements in Medicine and Biology, 2017, Vol. 44, pp. 74.
7. Emine S., Taki K., Cengiz E., Kafkas Univ. Vet. Fak. Derg, 2011, No. 17 (6), pp. 1031–1033.
8. Basanti J., Prakash K.V., Indian Journal of Animal Science, 2014, No. 84 (10), pp. 1133–1136.
9. Cinkulov M., Popovsky Z., Porcu K., Blagica T., J. Anim. Breed Genet, 2008, No. 125, pp. 417–426.
10. Elmaci C., One Y, Balcioglu M.S., J. Appl. Anim. Res, 2007, No. 32, pp. 145–148.
11. Elmaci C., One Y., Balcioglu M.S., Biochemical Genetics, 2006, No. 44, pp. 379–385.
12. Konovalova T.V., Sebezhko O.I., Li Wenrong, Proceedings of the International Symposium on animal science, 22nd-23rd November 2018 (ISAG), 22 University of Belgrade, Zenum, Belgrade, 47 p.
13. Petukhov V.L., Afonina I.A., Kleshchev M.A., Sebezhko O.I., Korotkevich O.S., Kostomakhin N.M., Konovalova T.V., Narozhnykh K.N., Adushinov D.S., Goncharenko G.M., Nezavitin A.G., Klimenok I.I., Nazarenko A.V., Klimanova E.A., Osadchuk L.V., Indian Journal of Ecology, 2019, No. 46 (4), pp. 901–906.
14. Wodas L., Mackowski M., PLoS ONE, 2020, No. 15 (4), pp. 1–14.
15. Andreeva V.A., Venrong Li, Mingzhun L’yu, Saurbaeva R.T., Konovalova T.V., Klimanova E.A., Sebezhko O.I., Nazarenko A.V., Vestnik NGAU, 2019, No. 4 (53), pp. 23–31. (In Russ.)
16. Narozhnykh K.M., Konovalova T.V., Petukhov V.L., International Journal of Advanced Biotechnology and Research (IJBR), 2016, Vol.7, Issue 4, pp. 1758–1764.
17. Tsygankova A.R., Kuptsov A.V., Narozhnykh K.N., Journal Pharm aceutical Sciences and Research, 2017, Vol. 9 (5), pp. 601–605.
18. Narozhnykh K. N., Konovalova T.V., Korotkevich O.S., Petukhov V.L., Sebezhko O.I., Sovremennye problemy nauki i obrazovaniya, 2014, No. 6, pp. 1447. (In Russ.)
19. Lyukhanov M. P., Korotkevich O.S., Sebezhko O.S., Yudin N.S., Sovremennye problemy nauki i obrazovaniya, 2014, No. 1, pp. 326. (In Russ.)
20. Lyukhanov M.P., Korotkevich O.S., Petukhov V.L., Sebezhko O.I., Glavnyi zootekhnik, 2014, No. 10, pp. 21–26. (In Russ.)
21. Saurbaeva R.T., Andreeva V.A., Piotrovskaya D.V. Sb. II Nacional’noj nauch. konf. Teoriya i practica sovremennoj agrarnoj nauki, 2019, pp. 349–351. (In Russ.)
22. Volnin A.A., Zaitsev S. Yu., Bagirov V.A., Gusev I.V., Veterinariya, zootekhniya i biotekhnologiya, 2015, No. 10, pp. 13–19. (In Russ.)
Review
For citations:
Klimanova E.A., Konovalova T.V., Andreeva V.A., Korotkevich O.S., Petukhov V.L., Nazarenko Yu.S. Association of β-lactoglobulin genotypes with some biochemical parameters of the blood of Romanov sheep. Bulletin of NSAU (Novosibirsk State Agrarian University). 2020;(4):82-87. (In Russ.) https://doi.org/10.31677/2072-6724-2020-57-4-82-87
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