BIOLOGICAL FEATURES AND RESPONSE OF SALMONELLA BACTERIA ISOLATED FROM POULTRY PRODUCTS TO ANTIBIOTICS

Salmonelloses, which are associated with products from clinically healthy animals that undergone veterinary and sanitary observations and examinations and are secondary-contaminated in the process, transportation, processing and marketing, are at the forefront of the list of emerging food zoonoses. The haphazard use of antibiotics in agriculture contributes to the selection of resistant clones of microorganisms. The high resistance of strains of salmonella isolated from poultry production poses a real risk of transmission through the food chain to humans, as well as being perpetuated and passed on to subsequent generations of bacteria, expanding geographical areas as a result of globalization. Separation of Salmonella microorganisms from poultry production was carried out in accordance with GOST 31468-2012, antigenic profile of isolated isolates was determined according to the classification of Kaufmann-White, sensitivity of microorganisms to antibacterial preparations - according to MUK 4.2.1890-04. In the study of 503 samples of poultry production 42 isolates of microorganisms of the genus Salmonella were isolated, which amounted to 8.35% of the number of samples studied. Sensitivity to antimicrobial agents showed that 42% of the isolates were resistant to tetracyclines and only 5-16% were resistant to antibiotics of the II generation of fluoroquinolones (ciprofloxacin, norfloxacin) and III generation of cephalosporins (cefotaxime, cefoxitin, ceftriaxone). The authors found out the change in resistance towards cephepium, ciprofloxacin and amoxicillin resistance in the secondary isolates of S. virhow. The study of biological properties, frequency of isolation and resistance to various AMF of Salmonella microorganisms is an integral part of the development of mechanisms for controlling the formation of resistance to antimicrobial agents.

Salmonella microorganisms, antigene profile, antibiotics, resistance to antibiotics, antimicrobial

1. Panin A.N., Komarov A.A., Kulikovskii A.V. Veterinariya i zootekhniya, 2017, No. 5, pp.18-24. (In Russ.)

2. Skitovich G.S., Shatrova N.B., Pruntova O.V. Veterinariya segodnya, 2018, No. 4, pp. 3-7. (In Russ.)

3. Klimov, A.A. Veterinariya, 2003, No. 3, pp. 9-11. (In Russ.)

4. Potekhin V.A., Rusaleev V. S. Veterinariya segodnya, 2016, No. 1, pp. 24-26. (In Russ.)

5. Zaikina E.N., Skvortsov V.N., Prisnyi A.A. Veterinariya Kubani, 2017, No. 2, pp. 24-27. (In Russ.)

6. Sagabieva, N.N. Jepizootologicheskij monitoring sal’monelleza krupnogo rogatogo skota v Kurskoj oblasti (Epizootological monitoring of cattle salmonellosis in the Kursk region), Extended abstract of candidate’s thesis, Kursk, 2004, 135 p.

7. Musejbu, M. A. Obezzarazhivanie mjasa, iskusstvenno obsemenennogo sal’monellami i kishechnoj palochkoj s pomoshh’ju SVCh jenergii mikrovolnovyh bytovyh pechej (Disinfection of meat artificially seeded with salmonella and E. coli using microwave energy from microwave ovens), Extended abstract of candidate’s thesis, Moscow, 1994, 16 p.

8. Salautin, V. V. Patomorfologija i differencial’naja diagnostika sal’monelleza ptic, vyzvannogo razlichnymi serovariantami vozbuditelja (Pathomorphology and differential diagnosis of avian salmonellosis caused by various serovariants of the pathogen), Extended abstract of candidate’s thesis, Saratov, 2004, 28 p.

9. Vitkovskaya, O. N. Edinyj mir - edinoe zdorov’e: Tezisy doklada (Proceeding of the VII International Veterinary Congress), Abstract of Papers, April, 2017, Ufa. (In Russ.)

10. Jacoby, G. L.,Archer G. L. New mechanisms of bacterial resistance to antimicrobial agents, New England Journal of Medicine, 1991, No. 9(324), pp. 601-612.

11. Keren I. Persisted cells and tolerance to antimicrobials, FEMS Microb. Lett, 2004, Vol. 230, pp. 13-18.

12. Race J.L., Biofilms, infection, and antimicrobial therapy, Boca Raton: Taylor & Francis Group, 2006, 495 p.

13. Borisenkova A.N. Zhivotnovodstvo Rossii, 2007, No. 5, pp. 18-24. (In Russ.)

14. Kondro W. Canadian scientists urge government to develop antibiotic plan, The Lancet, 2002, Vol. 360, 1230 p.

15. Midtvedt, T. Penicillins, cephalosporins and tetracyclines, Shn Dukes, M.N.G. (ed.), Side Effects of Drugs Annual 9, Amsterdam: Elsevier, 1985, 230 p.