APPROPRIATE CONDITIONS FOR CULTIVATING THE STRAIN OF NEWCASTLE DISEASE VIRUS IN THE DEVELOPING HEN EMBRYOS

Newcastle disease is observed and detected on all the continents of the globe, except Australia, and causes great economic losses in poultry production. In the Kyrgyz Republic, Newcastle disease outbreaks were observed in 2015 and 2016. Regardless Newcastle’s pathogen is well explored, there are specific features of disease course and the problem of infection elimination is not solved. The authors explored the appropriate conditions for growing Newcastle’s virus in developing hen embryos. For this the researchers infected chicken embryos with different doses: 10-1 to 10-8 viral propagation was applied for 10-day developing chicken embryos into the allantoid cavity in the volume of 0.1 and 0.2 cm3 (doses of 6,104 to 0.6 50% embryonic infectious dose (EID50 ) per embryo and 1.2-105 to 1.2 50% embryonic infectious dose (EID50 ) per embryo, respectively). The degree of virus accumulation was estimated by means of hemagglutination test according to the titers of hemagglutinins in the allantoic fluid. The titers of hemagglutinins in solutions within 10-1 to 10-8 of virus-containing material shows that at volume of an inoculated virus-containing material of 0,1 cm3 accumulation of hemagglutinins of a Newcastle virus strain occurs at high level and does not essentially differ (Р>0,5); when applied for infecting developing chicken embryos of virus cultivation to 10-6 (a dose in this case ~ 60 50 % an embryonic infectious dose EID50 ).

1. Kelneka B.U., Barnsa D.H., Biehrda Ch.U. Bolezni domashnih i sel’skohozyajstvennyh ptic (Diseases of domestic and farm birds), 2003, 1232 p.

2. Gough. R.E., Allan W.H. Aerosol vaccination against Newcastle disease using the Ulster strain, Avian Path., 1976, Vol.5, pp. 81–95.

3. Lam. K.M., Hao Q. Vaccination of cyclophosphamide-treated chickens against Newcastle disease virus interferon, Vet. Microbiol., 1994, No. 1–2 (45), pp. 41–48.

4. Newcastle disease vaccine on scratch feeds, World poultry, 1989, No. 8 (53), 12 p.

5. Newcastle disease, OIE Manual of Standards for Diagnostics Tests and Vaccines, in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Mammals, Birds and Bees, Office Internationaldes Epizootics, Paris, 2009, pp. 576–589, Chapter 2.3.14.

6. Meulmanns. G. Control by vaccination Newcastle disease, Boston: Kluver, 1988, pp. 318–332.

7. Miller. P.J., Kim. L.M., Ip. H.S., Afonso C.L. Evolutionary dynamics of Newcastle disease virus, Virology, 2009, Vol. 391, pp. 64–72.

8. Mihajlova V.V., Vitkova O.N., Belousova R.V. Izuchenie nekotoryh biologicheskih svojstv izolyatov virusa n’yukaslskoj bolezni, vydelennyh ot golubej (Study of some biological properties of Newcastle disease virus isolates isolated from pigeons), Proceeding of the Scientific and Practical Conference, Moscow: FGOU VPO MGAVMiB, 2006, pp. 20–23. (In Russ.)

9. Zubcova R.A., Ageeva L.S. Izuchenie immunobiologicheskih svojstv virus vakciny protiv psevdochumy ptic iz shtamma La-Sota, Aktual’nye voprosy veterinarnoj virusologii (Topical issues of veterinary virology), Proceeding of the National Conference, Moscow, 1967, Vol. 1, pp. 221–222. (In Russ.)

10. 1orguani G. EHffektivnost» razlichnyh metodov vakcinacii ptic protiv n’yukaslskoj bolezni, Veterinariya, 1990, No. 6, pp. 32–3.