vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian Universityvestngau-565Research ArticleБИОЛОГИЯ, ФИЗИОЛОГИЯ, ЭКОЛОГИЯBIOLOGY, PHYSIOLOGY AND ECOLOGYПОДХОДЫ К ОБНАРУЖЕНИЮ ГЕННО-МОДИФИЦИРОВАННОЙ КУКУРУЗЫ И ПРОВЕДЕНИЮ ПОЛУКОЛИЧЕСТВЕННОГО АНАЛИЗА С ИСПОЛЬЗОВАНИЕМ БЕЛКА СР4 EPSPSApproaches to investigation of gene modified maize and semi-quantitaive analysis by means of СР4 EPSPS proteinПоповскиЗ. .noemail@neicon.ruМискоска-МилевскаЭ. .noemail@neicon.ruНесторовскиТ. .noemail@neicon.ruКамалдиновЕ. В.noemail@neicon.ruПетуховВ. Л.vpetukhov@ngs.ruУниверситет св. Кирилла и Мефодия, Скопье, МакедонияRussian FederationНовосибирский государственный аграрный университетRussian Federation201631102016039297Copyright © Поповски З..., Мискоска-Милевска Э..., Несторовски Т..., Камалдинов Е.В., Петухов В.Л., 20162016Поповски З..., Мискоска-Милевска Э..., Несторовски Т..., Камалдинов Е.В., Петухов В.Л.Поповски З..., Мискоска-Милевска Э..., Несторовски Т..., Камалдинов Е.В., Петухов В.Л.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/565

Представлены результаты использования различных методов обнаружения генно-модифицированных организмов (ГМО) в пробах пищевых продуктов по уровню белка. В качестве исходного материала использованы семена генно-модифицированной кукурузы NK603 с разным содержанием ГМО, предоставленные Международной ассоциацией оценки семян (International Seed Testing Association - ISTA) в рамках программы оценки качества. Использовали специальные тест-полоски для проведения качественного и иммуноферментнного анализа (ИФА) для полуколичественного определения, где в качестве мишени использовали молекулы СР4 EPSPS белка. Результаты исследований показали, что с использованием тест-полосок можно обнаружить присутствие 0,6% ГМО. Показано, что порог чувствительности тест-полосок обнаруживается при температурной обработке образцов при 80°C. Установленная линейная регрессионная зависимость служит доказательством применимости предлагаемого подхода и использования ИФА для полуколичественного определения ГМО по белку СР4 EPSPS в образцах, содержащих ГМ-кукурузу с концентрацией 0,1%. Отмечаются незначительные отличия при использовании фильтров с разными длинами волн.

The article explores application of different methods aimed at finding gene-modified organisms (GMO) on concentration of protein in the samples of food products. As starting material, the researchers used seeds of gene-modified maize NK603 with different concentration of GMO represented by International Seed Testing Association (ISTA) in frames of the programme of quality assessment The researchers used specific test strips for qualitative and immunofluorescence assay (IFA) for semi-quantitative analysis where they used СР4 EPSPS molecules of protein. The research has shown that application of test stripes contributes to searching for 0.6 % of GMO. Sensation threshold of test stripes is found in temperature processing at 80 °C. The linear regressive correlation confirms ability to apply the suggested approach and immunofluorescence assay for semi-quantitative determination of GMO on protein СР4 EPSPS in the samples that contain GM-corn with concentration 0.1 %. The paper notes slight differences in application of filters with different waves.

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The authors declare that there are no conflicts of interest present.