vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian University10.31677/3033-8433-2026-21-1-78-86vestngau-2840Research ArticleАГРОНОМИЯAGRONOMYМолекулярный скрининг генов FaFAD1 и FanAAMT ароматического комплекса плодов земляники садовойMolecular screening of the FAFAD1 and FANAAMT genes of the strawberry fruit aromatic complexХудяковаА. В.KhudyakovaA. V.

кандидат биологических наук, научный сотрудник

Ижевск

candidate of biological sciences, researcher

Izhevsk

a.khudyakova@udman.ruУдмуртский федеральный исследовательский центр Уральского отделения Российской академии наукРоссияUdmurt Federal Research Center of the Ural Branch of the Russian Academy of SciencesRussian Federation202612082026017886Copyright © Худякова А.В., 20262026Худякова А.В.Khudyakova A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/2840

The content of volatile compounds in strawberries has a significant effect on the taste and fruit flavor. Most of the components of the strawberry fruit aromatic complex are controlled polygenically, while the biosynthesis of γ-decalactone and anthranilic acid methyl ester is controlled by the dominant FaFAD1 and FanAAMT genes. This study presents the results of a molecular genetic analysis of strawberry cultivars from the collection of the Department of plant introduction and acclimatization of the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences to identify genotypes promising for fruit flavor breeding. 34 cultivars from the Fragaria × ananassa Duch collection of various ecological and geographical origin were used as biological objects. Allelic diversity of the FaFAD1 and FanAAMT genes were identified by polymerase chain reaction using the corresponding dominant DNA markers. The functional allele of the FaFAD1 gene was detected in 26.5 % of the genotypes studied: 4 varieties of Russian cultivars (Akvarel’, Darenka, Sudarushka, Krasnaya pchelka) and 5 – foreign (Cabrillo, Cisojee, Corona, Malling Pandora, Murano). The FanAAMT gene was detected in 16 cultivars, which accounted for 47.1 % of the total sample. Among the identified genotypes, 9 belonged to Russian cultivars (Altyn, Gordost’ Urala, Darenka, Lyubasha, Pervoklassnitsa, Torpeda, Festival’naya, Feyerverk, Tsaritsa) and 7 to foreign varieties (Cabrillo, Cisojee, Kimberly, Malling Pandora, Marmion, Olvia, Salsa). The analysis of pedigrees of varieties with identified genes of the fruit aromatic complex of strawberry has been carried out. Among the studied collection, the content of two functional gene alleles was characterized by the varieties Darenka, Cabrillo, Cisojee, Malling Pandora, which amounted to 11.8 % of the cultivars. It is these genotypes that are the most promising for use in the fruit flavor breeding program.

The content of volatile compounds in strawberries has a significant effect on the taste and fruit flavor. Most of the components of the strawberry fruit aromatic complex are controlled polygenically, while the biosynthesis of γ-decalactone and anthranilic acid methyl ester is controlled by the dominant FaFAD1 and FanAAMT genes. This study presents the results of a molecular genetic analysis of strawberry cultivars from the collection of the Department of plant introduction and acclimatization of the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences to identify genotypes promising for fruit flavor breeding. 34 cultivars from the Fragaria × ananassa Duch collection of various ecological and geographical origin were used as biological objects. Allelic diversity of the FaFAD1 and FanAAMT genes were identified by polymerase chain reaction using the corresponding dominant DNA markers. The functional allele of the FaFAD1 gene was detected in 26.5 % of the genotypes studied: 4 varieties of Russian cultivars (Akvarel’, Darenka, Sudarushka, Krasnaya pchelka) and 5 – foreign (Cabrillo, Cisojee, Corona, Malling Pandora, Murano). The FanAAMT gene was detected in 16 cultivars, which accounted for 47.1 % of the total sample. Among the identified genotypes, 9 belonged to Russian cultivars (Altyn, Gordost’ Urala, Darenka, Lyubasha, Pervoklassnitsa, Torpeda, Festival’naya, Feyerverk, Tsaritsa) and 7 to foreign varieties (Cabrillo, Cisojee, Kimberly, Malling Pandora, Marmion, Olvia, Salsa). The analysis of pedigrees of varieties with identified genes of the fruit aromatic complex of strawberry has been carried out. Among the studied collection, the content of two functional gene alleles was characterized by the varieties Darenka, Cabrillo, Cisojee, Malling Pandora, which amounted to 11.8 % of the cultivars. It is these genotypes that are the most promising for use in the fruit flavor breeding program.

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The authors declare that there are no conflicts of interest present.