vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian University10.31677/2072-6724-2025-75-2-152-159vestngau-2601Research ArticleВЕТЕРИНАРИЯ, ЗООТЕХНИЯ И БИОТЕХНОЛОГИЯVETERINARY, ANIMAL SCIENCES AND BIOTECHNOLOGYВлияние разных источников углерода на продукцию биосурфактантов природными штаммами Bacillus spp.Effect of different carbon sources on biosurfactant production by natural strains of Bacillus spp.ДудникД. Е.DudnikD. E.

аспирант 

Барнаул

Postgraduate Student

Barnaul

dudnik-dina@mail.ruИркитоваА. Н.IrkitovaA. N.

кандидат биологических наук, доцент

Барнаул

Candidate of Biological Science

Barnaul

МалковаА. В.MalkovaA. V.

кандидат биологических наук

Барнаул

Candidate of Biological Science

Barnaul

КожевниковаЕ. Н.KozhevnikovaE. N.

лаборант-исследователь

Барнаул

Laboratory assistant

Barnaul

Алтайский государственный университетРоссияAltai State UniversityRussian Federation20251807202502152159Copyright © Дудник Д.Е., Иркитова А.Н., Малкова А.В., Кожевникова Е.Н., 20252025Дудник Д.Е., Иркитова А.Н., Малкова А.В., Кожевникова Е.Н.Dudnik D.E., Irkitova A.N., Malkova A.V., Kozhevnikova E.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/2601

Биосурфактанты – биотехнологически ценные метаболиты микроорганизмов. Эти соединения обладают не только поверхностно-активными свойствами, но и способностью к подавлению роста патогенных и условно-патогенных бактерий и грибов. Широкий спектр свойств, а также безопасность биосурфактантов позволили найти им применение в разных отраслях промышленности. Однако их производство в настоящее время проблематично. Основные проблемы при получении биосурфактантов связаны с поиском высокоактивных штаммов-продуцентов, оптимизацией состава сред для их культивирования и сокращением затрат на производство. Целью работы было изучить влияние разных источников углерода на продукцию биосурфактантов природными штаммами бацилл. Был проведен скрининг штаммов Bacillus spр. из коллекции Инжинирингового центра «Промбиотех», обладающих сурфактационной активностью. Для отобранных штаммов показано влияние сахарозы, глицерина, маннита и свекловичной мелассы на продукцию биосурфактантов. Установлено, что штамм Bacillus atrophaeus 7 способен к производству поверхностно-активных соединений только при культивировании на средах с мелассой в качестве единственного источника углерода. Напротив, для штамма Bacillus subtilis 1/8 зафиксирована продукция биосурфактантов при использовании сред с сахарозой, глицерином, маннитом и свекловичной мелассой. Также установлено влияние продолжительности культивирования на накопление сурфактантов в среде для обоих штаммов бацилл. Увеличение продолжительности роста приводило к снижению концентрации биосурфактантов в культуральной жидкости.

Biosurfactants are biotechnologically valuable metabolites of microorganisms. These compounds have not only surface-active properties, but also the ability to suppress growth of pathogenic and opportunistic bacteria and fungi. The wide range of characteristics and safety of biosurfactants have allowed them to find application in various industries. However, their production is currently problematic. The main problems in biosurfactants obtaining are associated with the search for highly active strains-producers, optimization of media composition for their cultivation, and production costs reduction. The aim of the work was to study the influence of different carbon sources on the biosurfactants production by natural bacilli strains. A screening of Bacillus spр. strains that possess surfactant activity from the Engineering Center “Prombiotech” collection was conducted. For the selected strains, the influence of sucrose, glycerol, mannitol and beet molasses on the biosurfactants production was shown. It was established that the Bacillus atrophaeus 7 strain is capable of producing surfaceactive compounds only when cultivated on media with molasses as the sole carbon source. On the contrary, for the Bacillus subtilis 1/8 strain, production of biosurfactants was recorded when using media with sucrose, glycerol, mannitol and beet molasses. The influence of cultivation duration on the surfactants accumulation in the medium for both bacilli strains was also established. The growth duration increase resulted in biosurfactants concentration decrease in the culture liquid.

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The authors declare that there are no conflicts of interest present.