vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian University10.31677/2072-6724-2024-71-2-259-269vestngau-2320Research ArticleВЕТЕРИНАРИЯ, ЗООТЕХНИЯ И БИОТЕХНОЛОГИЯVETERINARY, ANIMAL SCIENCES AND BIOTECHNOLOGYОсобенности гематологического статуса коров разных пород Западной СибириFeatures of hematological status of cows of different breeds of Western SiberiaСебежкоО. И.SebezhkoO. I.

Кандидат биологических наук, доцент.

Новосибирск

Candidate of Biological Sciences, Associate Professor.

Novosibirsk

КороткевичО. С.KorotkevichO. S.

Доктор биологических наук, профессор.

Новосибирск

Doctor of Biological Sciences, Professor.

Novosibirsk

КоноваловаТ. В.KonovalovaT. V.

Старший преподаватель.

Новосибирск

Senior lecturer.

Novosibirsk

ПетуховВ. Л.PetukhovV. L.

Доктор биологических наук, профессор.

Новосибирск

Doctor of Biological Sciences, Professor.

Novosibirsk

КочневН. Н.KochnevN. N.

Доктор биологических наук, профессор.

Новосибирск

Doctor of Biological Sciences, Professor.

Novosibirsk

СебежкоА. Н.SebezhkoA. N.

Студент.

Новосибирск

Student.

Novosibirsk

ТарасенкоЕ. И.TarasenkoE. I.

Аспирант.

Новосибирск

Phd student.

Novosibirsk

Новосибирский государственный аграрный университетРоссияNovosibirsk State Agrarian UniversityRussian FederationНовосибирский государственный технический университетРоссияNovosibirsk State Technical UniversityRussian Federation20241007202402259269Copyright © Себежко О.И., Короткевич О.С., Коновалова Т.В., Петухов В.Л., Кочнев Н.Н., Себежко А.Н., Тарасенко Е.И., 20242024Себежко О.И., Короткевич О.С., Коновалова Т.В., Петухов В.Л., Кочнев Н.Н., Себежко А.Н., Тарасенко Е.И.Sebezhko O.I., Korotkevich O.S., Konovalova T.V., Petukhov V.L., Kochnev N.N., Sebezhko A.N., Tarasenko E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/2320

Мониторинг гематологических параметров дойного стада занимает центральное место в оценке здоровья и благополучия животных. Изучены основные показатели периферической крови у здоровых коров голштинской, черно-пестрой и красной пород в возрасте 30–60 дня 2-й лактации, выращиваемых в Западной Сибири. Исследования эритроцитарных, лейкоцитарных и тромбоцитарных параметров проводились на анализаторе PCЕ 90Vet с микроскопией мазков для подсчета лейкоцитарной формулы. Использовали стандартные методы описательной статистики или робастные статистики. Межгрупповые сравнения проводили с помощью однофакторного дисперсионного анализа или критерия Краскера – Уоллеса. Для апостериорных сравнений использовали метод Тьюки или Данна. При расчете референсных интервалов пользовались бутстреп-методом. Рассчитанные средние значения эритроцитарного, тромбоцитарного и лейкоцитарного звеньев гемограммы в большинстве случаев варьировали в пределах физиологических норм или находились на границе пределов. Наиболее выраженным адаптационным потенциалом харатеризовались животные голштинской породы. Установлены различия между породами практически по всем изученным показателям кроме числа эритроцитов, MCV, RDW, базофилов, палочкоядерных нейтрофилов, индекса иммунореактивности. Для коров черно-пестрой породы был характерен наиболее высокий уровень гематокрита (27,99±0,31 %), абсолютного числа эозинофилов (Me = 0,207 ×109/л), тромбоцитов (279,5±22,13 ×109/л), для красной степной – гемоглобина (98,32±1,53 г/л), моноцитов (0,29±0,01) и среднего объема тромбоцитов (6,42±0,08 фл). Определенные референсные интервалы лейкоцитарного профиля коров обеспечивает мониторинг состояния здоровья. Он дает возможность сравнения данных с другими породами, а также позволяет вести сравнительный мониторинг между регионами.

Monitoring hematological parameters in dairy herds is central to assessing animal health and welfare. The main parameters of peripheral blood were studied in healthy cows of Holstein, black-and-white and red breeds at the age of 30-60 days of the 2nd lactation, raised in Western Siberia. Studies of erythrocyte, leukocyte and platelet parameters were carried out on a PCE 90Vet analyzer with smear microscopy for calculating the leukocyte formula. Standard methods of descriptive statistics or robust statistics were used. Intergroup comparisons were performed using one-way analysis of variance or the Krasker-Wallace test. Tukey or Dunn’s method was used for post hoc comparisons. When calculating reference intervals, we used the bootstrap method. The calculated average values of the erythrocyte, platelet and leukocyte components of the hemogram in most cases varied within physiological norms or were on the borderline. Animals of the Holstein breed had the most pronounced adaptive potential. Differences between breeds have been established in almost all studied indicators except the number of red blood cells, MCV, RDW, basophils, band neutrophils, and immunoreactivity index. Cows of the black-andwhite breed were characterized by the highest level of hematocrit (27.99 ± 0.31%), absolute number of eosinophils (Me = 0.207 ×109/l), platelets (279.5 ± 22.13 ×109/l), for red steppe hemoglobin (98.32±1.53 g/l), monocytes (0.29±0.01) and average platelet volume (6.42±0.08 fL). Certain reference intervals for the leukocyte profile of cows provide monitoring of health status, the ability to compare data with other breeds, and also allows for comparative monitoring between regions.

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The authors declare that there are no conflicts of interest present.