vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian University10.31677/2072-6724-2023-66-1-157-170vestngau-1932Research ArticleВЕТЕРИНАРИЯ И ЗООТЕХНИЯVETERINARY MEDICINE AND ANIMAL SCIENCEНекоторые аспекты управления репродукционной функцией у самок осетровых рыбSome aspects of the control of the reproduction function in female sturgeon fishesПономареваЕ. Н.PonomarevaE. N.

Е.Н. Пономарева, доктор биологических наук, профессор 

Ростов-на-Дону

E.N. Ponomareva, Doctor of Biological Sciences, Professor 

Rostov-on-Don

kafavb@mail.ruГераскинП. П.GeraskinP. P.

П.П. Гераскин, доктор биологических наук 

Астрахань

P.P. Geraskin, Doctor of Biological Sciences 

Astrakhan

СорокинаМ. Н.SorokinaM. N.

М.Н. Сорокина, кандидат биологических наук, доцент 

Ростов-на-Дону

M.N. Sorokina, PhD in Biological Sciences, Associate Professor 

Rostov-on-Don

ГригорьевВ. А.GrigorievV. A.

В.А. Григорьев, кандидат биологических наук 

Ростов-на-Дону

V.A. Grigoriev, PhD in Biological Sciences 

Rostov-on-Don

КовалёваА. В.KovalevaA. V.

А.В. Ковалёва, кандидат биологических наук 

Ростов-на-Дону

A.V. Kovaleva, PhD in Biological Sciences 

Rostov-on-Don

Федеральный исследовательский центр Южный научный центр Российской академии наукРоссияFederal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences (SSC RAS)Russian FederationАстраханский государственный технический университетРоссияAstrakhan State Technical UniversityRussian Federation20231104202301157170Copyright © Пономарева Е.Н., Гераскин П.П., Сорокина М.Н., Григорьев В.А., Ковалёва А.В., 20232023Пономарева Е.Н., Гераскин П.П., Сорокина М.Н., Григорьев В.А., Ковалёва А.В.Ponomareva E.N., Geraskin P.P., Sorokina M.N., Grigoriev V.A., Kovaleva A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/1932

Проведены две серии экспериментов по влиянию препарата Е-селен на репродуктивную функцию осетровых рыб с разными способами внедрения препарата в организм: через корм и инъецированием. Показана эффективность действия Е-селена в двух сериях экспериментов. Выявлена его способность ускорять процесс созревания ооцитов вследствие стимуляции генеративного обмена и приведения его в соответствие с этапами полового цикла исходя из биохимических показателей крови. На усиление генеративного обмена указывает повышение концентрации в крови в 1,4 раза к концу 50-суточного эксперимента общего белка и бета-липопротеидов, в состав которых в период вителлогенеза входит ововителин (вителлогенин), а также умеренное увеличение концентрации гемоглобина. Отмечено, что обмен веществ у контрольных рыб в большей мере, чем у экспериментальных, направлен на соматический рост, вследствие чего средняя масса самок в контроле была выше, чем опыте, в первой серии экспериментов в 1,5 раза при неизменной массе опытных рыб и увеличении на 6% у контрольных во второй серии. Отмечена роль препарата как стабилизатора физиологического состояние самок из-за его высоких антиоксидантных свойств. При его применении исчезают имеющиеся признаки стрессового состояния, в отличие от контрольных рыб, у которых они выявляются: повышенный уровень холестерина, скорость оседания эритроцитов (СОЭ), снижение концентрации гемоглобина. Наибольший эффект от применения препарата получен при орошении Е-селеном корма, при этом количество созревших рыб после 50 суток эксперимента было в 2,7 раза больше, чем в контроле, с одновременным снижением в 2 раза числа незрелых рыб. В случае инъецирования самок этим препаратом количество рыб, находящихся на IV стадии зрелости гонад, у экспериментальной группы по истечении 50 суток увеличилось в 1,7 раза, у контрольных, наоборот, снизилось в 1,2 раза при неизменной доле самок на III стадии зрелости гонад.

Two series of experiments on the effect of E-selenium on the reproductive function of sturgeons were conducted with different ways of introducing the drug into the body: through feed and injection. The effectiveness of the action of E-selenium was shown in two experiments. First, its ability to accelerate the process of maturation of oocytes was revealed due to the stimulation of generative metabolism and bringing it into line with the stages of the sexual cycle based on the biochemical parameters of the blood. An increase in the concentration in the blood by 1.4 times by the end of the 50-day experiment of total protein and beta-lipoproteins, which include ovovitelin (vitellogenin) during the period of vitellogenesis, as well as a moderate increase in haemoglobin concentration, indicates an increase in generative metabolism. It was noted that the metabolism of control fish was directed to somatic growth to a greater extent than that of experimental fish, as a result of which the average weight of females in control was 1.5 times higher than in the first series of experiments and weight of experimental fish was the same and increased by 6% in power in the second series. The role of the drug as a stabilizer of the physiological state of females, due to its high antioxidant properties, has been noted. When used, the existing signs of a stress state disappear, in contrast to the control fish in which they are detected: an increased cholesterol level, an erythrocyte sedimentation rate (ESR), and a decrease in haemoglobin concentration. The most significant effect from the use of the drug was obtained when the feed was irrigated with E-selenium, in which the number of mature fish after 50 days of the experiment was 2.7 times greater than in control, with a simultaneous halving of immature fish. In the case of the injection of females with this preparation, the number of fish at the IV stage of gonadal maturity in the experimental group after 50 days increased by 1.7 times. In the control group, on the contrary, it decreased by 1.2 times, with a constant proportion of females by the III stage of gonadal maturity.

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The authors declare that there are no conflicts of interest present.