vestngauВестник университета биотехнологийVestnik University of biotechnologiy3033-8433Publishing Centre “Zolotoy Kolos” of Novosibirsk State Agrarian University10.31677/2072-6724-2020-54-1-48-55vestngau-1295Research ArticleВЕТЕРИНАРИЯ И ЗООТЕХНИЯVETERINARY MEDICINE AND ANIMAL SCIENCEЗАВИСИМОСТЬ УРОВНЯ ИНФИЦИРОВАННОСТИ САЛЬМОНЕЛЛАМИ В ПОПУЛЯЦИЯХ КУР ОТ АНТАГОНИСТИЧЕСКОЙ АКТИВНОСТИ LACTOBACILLACEAE И ENTEROCOCCACEAE В ОТНОШЕНИИ SALMONELLA ENTERICASALMONELLA INFECTION LEVEL IN CHICKEN POPULATIONS VERSUS ANTAGONISTIC ACTIVITY OF LACTOBACILLACEAE AND ENTEROCOCCACEAE AGAINST SALMONELLA ENTERICAАфонюшкинВ. Н.AfonyushkinV. N.

кандидат биологических наук

Candidate of Biological Sciences

ДавыдоваН. В.DavydovaN. V.

кандидат ветеринарных наук

Candidate of Veterinary Sciences

ТроменшлегерИ. Н.TromenschlegerI. N.

инженер

Engineer

МишуковаО. В.MishukovaO. V.

младший научный сотрудник

Junior Researcher

КозловаЮ. Н.KozlovaY. N.

кандидат биологических наук

Candidate of Biological Sciences

ЧерепушкинаВ. С.CherepushkinaV. S.

младший научный сотрудник

Junior Researcher

МироноваТ. Е.MironovaT. E.

лаборант-исследователь

Research Assistant

КлемешоваИ. Ю.KlemeshovaI. Y.

кандидат сельскохозяйственных наук

Candidate of Agricultural Sciences

klemeshova-inna@mail.ruСибирский федеральный научный центр агробиотехнологий РАН Новосибирск; Институт химической биологии и фундаментальной медицины СО РАНРоссияSiberian Federal Scientific Center for Agrobiotechnology RAS Novosibirsk; Institute of Chemical Biology and Fundamental Medicine SB RASRussian FederationСибирский федеральный научный центр агробиотехнологий РАН НовосибирскРоссияSiberian Federal Scientific Center for Agrobiotechnology RAS NovosibirskRussian FederationИнститут химической биологии и фундаментальной медицины СО РАНРоссияInstitute of Chemical Biology and Fundamental Medicine SB RASRussian FederationИнститут химической биологии и фундаментальной медицины СО РАН; Новосибирский государственный аграрный университетРоссияInstitute of Chemical Biology and Fundamental Medicine SB RAS; Novosibirsk State Agrarian UniversityRussian FederationНовосибирский государственный аграрный университетРоссияNovosibirsk State Agrarian UniversityRussian Federation202021032020014855Copyright © Афонюшкин В.Н., Давыдова Н.В., Троменшлегер И.Н., Мишукова О.В., Козлова Ю.Н., Черепушкина В.С., Миронова Т.Е., Клемешова И.Ю., 20202020Афонюшкин В.Н., Давыдова Н.В., Троменшлегер И.Н., Мишукова О.В., Козлова Ю.Н., Черепушкина В.С., Миронова Т.Е., Клемешова И.Ю.Afonyushkin V.N., Davydova N.V., Tromenschleger I.N., Mishukova O.V., Kozlova Y.N., Cherepushkina V.S., Mironova T.E., Klemeshova I.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestngau.elpub.ru/jour/article/view/1295

The antagonistic activity of lactobacilli in the intestine in relation to various enteropathogenic microorganisms can vary within wide limits, including depending on the species composition of the lactobiota of the intestine. The purpose of this work was to determine the antagonistic activity of representatives of the order Lactobacillales isolated from chickens in poultry farms with different levels of Salmonella infection. The test object was the chickens of the parent herd and broiler chickens of crosses Ross 308 and Hubbard F - 15 from five poultry farms. Three poultry farms were characterized by a low level of salmonella infection in birds (less than 5% for cloacal swabs in PCR and the absence of salmonella isolation from food products). Two poultry farms were characterized by a high level of Salmonella infection (poultry infection by cloacal swabs of more than 10% and official salmonellosis disadvantage due to isolation of Salmonella cultures in food products). The level of infection was evaluated by real-time PCR after preliminary subculture of cloacal swabs on Shadler’s broth. The antagonistic activity of lactobacilli and related bacterial species isolated from the same chickens was carried out in co-cultivation tests on the Shadler broth with subsequent identification of salmonella on the RVS broth. Poultry farms with low Salmonella infection were characterized by the presence of L. reuteri as a major component of intestinal lactobiota and had a higher antagonistic activity against more Salmonella cultures (odds ratio (OR) 17.33 (CI 95 = 5.99-50.07776))

лактобактериисальмонеллаантагонистическая активностькурыlactobacillisalmonellaantagonistic activityhensReferencesПавлова Н. В., Киржаев Ф. С., Лапинскайте Р. Значение нормальной микрофлоры пищеварительного тракта птиц для их организма // БИО. – 2002. – № 1. – С. 4–8.Pavlova N. V., Kirzhaev F. S., Lapinskajte R. BIO, 2002, No 1, pp. 4–8. (In Russ.)Успешный выбор кормовых добавок для сельскозяйственной птицы зависит от точного знания микрофлоры кишечника / Г. Ю. Лаптев, Л. А. Ильина, К. В. Нагорнова [и др.] // Intern. conf. «High-throughput sequencing in genomics». Novosibirsk. July 21–25. – 2013. – P. 43.Laptev G.YU., Il’ina L.A, Nagornova K. V., Nikonov I. N., Novikova N. I. High-throughput sequencing in genomics, Proceeding of Intern. Conference, Novosibirsk. July 21–25, 2013, 43 p. (In Russ.)Биологическая характеристика штаммов лактобацилл, перспективных в качестве эубиотиков/ Е. М. Горская, Н. Н. Лизько, А. А. Ленцнер [и др.] // Журн. микробиологии, эпидемиологии и иммунологии. – 1992. – № 3. – С. 17–20.Gorskaya E. M., Liz’ko N.N., Lencner A. A., Bondarenko V. M., Sokolova K. Ya., Lihacheva A. Yu. Zhurn. mikrobiol., epidemiol. i immunol, 1992, No 3, pp. 17–20. (In Russ.)Антагонистическая активность лактобактерий из кишечника сельскохозяйственной птицы в отношении клинических изолятов Salmonella enterica / В. Н. Афонюшкин, И. Н. Троменшлегер, М. Л. Филипенко [и др.], // Бюл. экспериментальной биологии и медицины. – 2016. – № 6. – С. 757–760.Afonyushkin V. N., Tromenshleger I. N., Filipenko M. L., Hrapov E. A., Dudareva E. V. Byulleten» eksperimental’noj biologii i mediciny, 2016, No 6, pp. 757–760. (In Russ.)Casas I.A, Dobrogosz W. J. Lactobacillus reuteri: an overview of a new probiotic for humans and animals // Microecol. Therap. – 1997. – Vol. 25. – P. 221–31.Casas I.A, Dobrogosz W. J. Lactobacillus reuteri, Microecol. Therap., 1997, Vol 25, pp. 221–31.Механизмы биологической активности системы Lactobacillus reuteri – реутерин / В. Н. Афонюшкин, М. Л. Филипенко, А. Н. Ширшова [и др.] //Сиб. вестн. с-х науки. – 2013. – № 4. – С. 70–75.Afonyushkin V. N., Filipenko M. L., Shrshova A. N., Maslov O. G. Sibirskij vestnik sel’skohozyajstvennoj nauki, 2013, No 4, pp. 70–75. (In Russ.)Молекулярно-биологические методы контроля сальмонеллезов: метод. рекомендации / В. Н. Афонюшкин, Т. В. Сподырева, Ю. Г. Юшков, В. Ю. Коптев. – Новосибирск, 2011. – 63 с.Afonyushkin V. N., Spodyreva T. V., Yushkov Yu.G., Koptev V. Yu. Molecular biological methods for the control of salmonella, Novosibirsk, 2011, 63 p.Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri / T. L. Talarico, I. A. Casas, T. C. Chung, W. J. Dobrogosz // Antimicrob. Agents Chemother. – 1988. – Vol. 32, N 12. – P. 1854–1858.Talarico T. L., Casas I. A., Chung T. C., Dobrogosz, W. J. Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri, Antimicrob. Agents Chemother, 1988, No 12 (32), pp. 1854–1858.Zhang D., Li R., Li J. Lactobacillus reuteri ATCC 55730 and L22 display probiotic potential in vitro and protect against Salmonella-induced pullorum disease in a chick model of infection // Res. Vet. Sci. – 2012. – Vol. 93, N. 1. – P. 366–373.Zhang, D., Li, R., and Li, J. Lactobacillus reuteri ATCC 55730 and L22 display probiotic potential in vitro and protect against Salmonella-induced pullorum disease in a chick model of infection, Res. Vet. Sci., 2012, No 1 (93), pp. 366–73.Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production / H. Morita, H. Toh, S. Fukuda [et al.] // DNA Res. – 2008. – Vol. 15, N 3. – P. 151–161.Morita, H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O’Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production, DNA Res., 2008, No 3 (15), pp. 151–161.The antimicrobial compound reuterin (3-hydroxypropionaldehyde) induces oxidative stress via interaction with thiol groups / L. Schaefer, T. A. Auchtung, K. E. Hermans [et al.] // Microbiology. – 2010. – Vol. 156, N. Pt 6. – P. 1589–1599.Schaefer L., Auchtung T. A., Hermans K. E., Whitehead D., Borhan B., Britton R. A. The antimicrobial compound reuterin (3-hydroxypropionaldehyde) induces oxidative stress via interaction with thiol groups, Microbiology, 2010, No Pt 6 (156), pp. 1589–99.Inhibitory activity of reuterin, nisin, lysozyme and nitrite against vegetative cells and spores of dairy-related Clostridium species/ M. Avila, N. Gomez-Torres, M. Hernandez, S. Garde // Int. J. Food Microbiol. – 2014. – Vol. 172. – P. 70–75.Avila M., Gomez-Torres N., Hernandez M., Garde, S. Inhibitory activity of reuterin, nisin, lysozyme and nitrite against vegetative cells and spores of dairy-related Clostridium species, Int. J. Food Microbiol, 2014, Vol 172, pp. 70–75.In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications/ I. Sulemankhil, M. Parent, M. L. Jones [et al.] // Can. J. Microbiol. – 2012. – Vol. 58, N 6. – P. 776–87.Sulemankhil I., Parent M., Jones M. L., Feng Z., Labbe A., Prakash S. In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications, Can. J. Microbiol, 2012, No 6 (58), pp. 776–87.Production of a Broad Spectrum Antimicrobial Substance by Lactobacillus reuteri / T. Axelsson, T. Chung, T. Dobrogosz, S. Lindgren // Microb. Ecol. Health Dis. – 1989. – Vol. 2, N 2. – P. 131–136.Axelsson T., Chung T., Dobrogosz T., Lindgren S. Production of a Broad Spectrum Antimicrobial Substance by Lactobacillus reuteri, Microb. Ecol. Health Dis., 1989, No 2 (2), pp. 131–136.Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens/ K. Ghareeb, W. A. Awad, M. Mohnl [et al.] // Poult. Sci. – 2012. – Vol. 91, N 8. – P. 1825–32.Ghareeb K., Awad W. A., Mohnl M. Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens, Poult. Sci., 2012, No 8 (91), pp. 1825–1832.Современные методы контроля сальмонеллёза/ В. Н. Афонюшкин, Е. В. Дударева, Л. И. Малахеева [и др.] // Птицеводство. – 2008. – № 9. – С. 43–44.Afonyushkin V. N., Dudareva E. V., Malaheeva L.I, Frolova O. V., Shkred O. V., Filipenko M. L., Sovremennye metody kontrolya sal’monellyoza, Pticevodstvo, 2008, No 9, pp. 43–44. (In Russ.)Chemically induced mouse models of intestinal inflammation/ S. Wirtz, C. Neufert, B. Weigmann, M. F. Neurath// Nat. Protoc. – 2007. – Vol. 2. – P. 541–546.Wirtz S., Neufert C., Weigmann B., Neurath M. F., Chemically induced mouse models of intestinal inflammation, Nat. Protoc., 2007, Vol 2, pp. 541–546.

The authors declare that there are no conflicts of interest present.